Zinc in PDB 2rax: Crystal Structure of Borealin (20-78) Bound to Survivin (1-120)

The structure of Crystal Structure of Borealin (20-78) Bound to Survivin (1-120), PDB code: 2rax was solved by S.G.Hymowitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.63 / 3.30
Space group	I 41 2 2
Cell size a, b, c (Å), α, β, γ (°)	145.521, 145.521, 217.429, 90.00, 90.00, 90.00
R / Rfree (%)	23.6 / 28.4

The binding sites of Zinc atom in the Crystal Structure of Borealin (20-78) Bound to Survivin (1-120) (pdb code 2rax). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of Borealin (20-78) Bound to Survivin (1-120), PDB code: 2rax:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of Borealin (20-78) Bound to Survivin (1-120)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Borealin (20-78) Bound to Survivin (1-120) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn341 b:64.4 occ:1.00 SG A:CYS84 2.1 65.6 1.0 SG A:CYS60 2.1 62.4 1.0 SG A:CYS57 2.4 52.4 1.0 NE2 A:HIS77 2.5 96.1 1.0 CB A:CYS84 3.0 65.8 1.0 CB A:CYS60 3.0 59.6 1.0 CE1 A:HIS77 3.1 95.3 1.0 CB A:CYS57 3.5 54.3 1.0 CD2 A:HIS77 3.7 96.0 1.0 N A:CYS60 3.7 61.8 1.0 CD1 A:LEU87 3.9 77.8 1.0 CA A:CYS60 4.0 58.8 1.0 OG A:SER81 4.3 85.7 1.0 ND1 A:HIS77 4.3 95.5 1.0 CA A:CYS84 4.4 68.8 1.0 CB A:PHE86 4.5 71.0 1.0 CG A:HIS77 4.6 95.5 1.0 CB A:PHE59 4.7 61.8 1.0 CB A:SER81 4.7 83.2 1.0 C A:PHE59 4.7 63.1 1.0 C A:CYS60 4.9 55.7 1.0 CA A:CYS57 4.9 59.6 1.0 CD2 A:PHE86 4.9 75.3 1.0 C A:CYS84 5.0 68.4 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of Borealin (20-78) Bound to Survivin (1-120)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Borealin (20-78) Bound to Survivin (1-120) within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn341 b:79.3 occ:1.00 NE2 E:HIS77 2.1 0.2 1.0 SG E:CYS57 2.2 73.5 1.0 SG E:CYS84 2.2 82.2 1.0 SG E:CYS60 2.4 65.8 1.0 CE1 E:HIS77 2.8 0.4 1.0 CB E:CYS84 3.0 84.3 1.0 CB E:CYS60 3.1 69.1 1.0 CB E:CYS57 3.1 73.9 1.0 CD2 E:HIS77 3.2 0.7 1.0 N E:CYS60 3.5 71.8 1.0 CA E:CYS60 3.9 71.1 1.0 ND1 E:HIS77 4.0 0.9 1.0 CG E:HIS77 4.2 0.5 1.0 CA E:CYS84 4.4 86.1 1.0 CB E:PHE59 4.5 70.0 1.0 CA E:CYS57 4.6 75.1 1.0 C E:PHE59 4.6 72.8 1.0 CB E:PHE86 4.7 88.6 1.0 OG E:SER81 4.7 0.0 1.0 C E:CYS60 4.9 73.9 1.0 CA E:PHE59 4.9 71.3 1.0 N E:PHE61 5.0 76.9 1.0 N E:PHE59 5.0 72.6 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of Borealin (20-78) Bound to Survivin (1-120)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Borealin (20-78) Bound to Survivin (1-120) within 5.0Å range: probe atom residue distance (Å) B Occ X:Zn341 b:87.7 occ:1.00 NE2 X:HIS77 2.2 0.4 1.0 SG X:CYS57 2.2 98.0 1.0 SG X:CYS84 2.3 0.2 1.0 SG X:CYS60 2.4 97.7 1.0 CE1 X:HIS77 2.9 0.5 1.0 CB X:CYS84 3.1 0.8 1.0 CD2 X:HIS77 3.3 0.4 1.0 CB X:CYS57 3.3 96.9 1.0 CB X:CYS60 3.5 96.1 1.0 N X:CYS60 3.8 99.1 1.0 ND1 X:HIS77 4.1 0.2 1.0 CA X:CYS60 4.2 97.0 1.0 CG X:HIS77 4.3 0.6 1.0 CB X:PHE86 4.5 0.7 1.0 CB X:PHE59 4.5 93.2 1.0 CA X:CYS84 4.5 0.7 1.0 CD1 X:LEU87 4.6 0.6 1.0 CA X:CYS57 4.7 98.2 1.0 C X:PHE59 4.8 98.7 1.0 OG X:SER81 4.9 0.8 1.0 C X:CYS60 4.9 95.9 1.0 N X:PHE59 5.0 93.3 1.0 CA X:PHE59 5.0 94.2 1.0

E.Bourhis, S.G.Hymowitz, A.G.Cochran. The Mitotic Regulator Survivin Binds As A Monomer to Its Functional Interactor Borealin. J.Biol.Chem. V. 282 35018 2007.
ISSN: ISSN 0021-9258
PubMed: 17881355
DOI: 10.1074/JBC.M706233200