Zinc in PDB 2ori: Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/)

All present enzymatic activity of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/):
2.7.4.3;

The structure of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/), PDB code: 2ori was solved by R.Counago, Y.Shamoo, C.J.Wilson, G.Wu, J.Myers, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	34.694, 75.136, 77.523, 90.00, 98.37, 90.00
R / Rfree (%)	22.8 / 26.9

The structure of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/) also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/) (pdb code 2ori). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/), PDB code: 2ori:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn420 b:17.9 occ:1.00 OD2 A:ASP153 2.1 13.7 1.0 SG A:CYS130 2.3 12.3 1.0 SG A:CYS150 2.3 14.7 1.0 SG A:CYS133 2.4 18.6 1.0 CG A:ASP153 2.8 16.8 1.0 OD1 A:ASP153 2.9 14.2 1.0 CB A:CYS130 3.1 13.2 1.0 CB A:CYS150 3.2 12.4 1.0 CB A:CYS133 3.3 19.4 1.0 N A:CYS133 3.6 19.3 1.0 CA A:CYS133 4.0 19.0 1.0 O A:HOH520 4.2 32.8 1.0 CB A:ASP153 4.3 16.8 1.0 CB A:VAL132 4.4 20.0 1.0 CG2 A:THR135 4.4 19.2 1.0 N A:ASP153 4.4 18.7 1.0 CA A:CYS130 4.5 12.6 1.0 CB A:LYS152 4.5 20.2 1.0 C A:VAL132 4.6 20.2 1.0 CA A:CYS150 4.7 15.6 1.0 C A:CYS133 4.7 17.9 1.0 N A:GLY134 4.8 16.3 1.0 CG1 A:VAL132 4.8 20.4 1.0 CA A:VAL132 4.9 19.6 1.0 N A:VAL132 4.9 18.6 1.0 CA A:ASP153 5.0 16.8 1.0 CD A:LYS152 5.0 25.8 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn421 b:26.1 occ:1.00 OD2 B:ASP153 2.2 21.9 1.0 SG B:CYS130 2.3 21.0 1.0 SG B:CYS150 2.3 21.2 1.0 SG B:CYS133 2.4 25.6 1.0 CG B:ASP153 2.9 23.4 1.0 OD1 B:ASP153 2.9 20.9 1.0 CB B:CYS130 3.1 21.2 1.0 CB B:CYS150 3.2 20.1 1.0 CB B:CYS133 3.4 24.1 1.0 N B:CYS133 3.6 22.9 1.0 CG2 B:THR135 4.0 21.9 1.0 CA B:CYS133 4.1 23.3 1.0 CB B:ASP153 4.3 22.6 1.0 O B:HOH547 4.3 29.5 1.0 CB B:VAL132 4.4 22.1 1.0 CB B:LYS152 4.5 24.4 1.0 N B:ASP153 4.5 23.8 1.0 CA B:CYS130 4.6 20.4 1.0 CA B:CYS150 4.6 20.7 1.0 C B:VAL132 4.7 23.5 1.0 CD B:LYS152 4.7 27.0 1.0 C B:CYS133 4.8 23.3 1.0 N B:GLY134 4.8 22.7 1.0 CG1 B:VAL132 4.9 23.5 1.0 CA B:VAL132 4.9 22.9 1.0 N B:VAL132 4.9 22.8 1.0

R.Counago, Y.Shamoo, C.J.Wilson, G.Wu, J.Myers, P.Wittung-Stafshede. Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (A193V/Q199R/) To Be Published.