Zinc in PDB 2oo7: Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R)

All present enzymatic activity of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R):
2.7.4.3;

The structure of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R), PDB code: 2oo7 was solved by R.Counago, C.J.Wilson, G.Wu, J.C.Myers, P.Wittung-Stafshede, Y.Shamoo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 1.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	34.810, 75.760, 77.460, 90.00, 98.15, 90.00
R / Rfree (%)	19.7 / 24.5

The structure of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R) also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R) (pdb code 2oo7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R), PDB code: 2oo7:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn920 b:16.1 occ:1.00 OD2 A:ASP153 2.2 17.0 1.0 SG A:CYS130 2.4 12.9 1.0 SG A:CYS133 2.4 14.8 1.0 SG A:CYS150 2.4 13.4 1.0 OD1 A:ASP153 2.8 20.0 1.0 CG A:ASP153 2.8 20.6 1.0 CB A:CYS130 3.1 11.9 1.0 CB A:CYS150 3.2 12.0 1.0 CB A:CYS133 3.3 14.9 1.0 N A:CYS133 3.6 14.9 1.0 CA A:CYS133 4.0 14.7 1.0 CB A:ASP153 4.3 21.7 1.0 CG2 A:THR135 4.3 14.9 1.0 N A:ASP153 4.4 20.3 1.0 CB A:VAL132 4.4 15.0 1.0 CB A:LYS152 4.5 19.6 1.0 CA A:CYS130 4.6 10.8 1.0 C A:VAL132 4.6 15.5 1.0 CA A:CYS150 4.7 12.8 1.0 C A:CYS133 4.8 14.5 1.0 N A:GLY134 4.8 13.2 1.0 CA A:VAL132 4.9 14.8 1.0 CA A:ASP153 4.9 19.5 1.0 CD A:LYS152 4.9 22.1 1.0 N A:VAL132 4.9 12.6 1.0 CG1 A:VAL132 5.0 14.6 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn921 b:14.9 occ:1.00 OD2 B:ASP153 2.2 13.8 1.0 SG B:CYS130 2.4 12.2 1.0 SG B:CYS133 2.4 13.0 1.0 SG B:CYS150 2.4 14.2 1.0 OD1 B:ASP153 2.7 14.7 1.0 CG B:ASP153 2.8 15.1 1.0 CB B:CYS150 3.1 13.1 1.0 CB B:CYS130 3.1 10.6 1.0 CB B:CYS133 3.3 12.3 1.0 N B:CYS133 3.6 13.2 1.0 CG2 B:THR135 3.9 16.9 1.0 CA B:CYS133 4.0 12.8 1.0 CB B:ASP153 4.2 14.9 1.0 O B:HOH1279 4.3 14.3 1.0 CB B:VAL132 4.3 15.5 1.0 N B:ASP153 4.4 16.6 1.0 CB B:LYS152 4.5 16.3 1.0 CA B:CYS130 4.6 12.0 1.0 C B:VAL132 4.6 14.3 1.0 CA B:CYS150 4.6 12.9 1.0 C B:CYS133 4.8 12.9 1.0 N B:GLY134 4.8 12.9 1.0 CD B:LYS152 4.8 20.6 1.0 CA B:VAL132 4.8 14.5 1.0 CG1 B:VAL132 4.9 15.3 1.0 N B:VAL132 4.9 14.1 1.0 CA B:ASP153 4.9 16.1 1.0 CE B:LYS152 4.9 23.6 1.0

R.Counago, C.J.Wilson, G.Wu, J.C.Myers, P.Wittung-Stafshede, Y.Shamoo. Crystal Structure of A Thermostable Mutant of Bacillus Subtilis Adenylate Kinase (T179I/Q199R) To Be Published.