Zinc in PDB 2occ: Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State

All present enzymatic activity of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State:
1.9.3.1;

The structure of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State, PDB code: 2occ was solved by T.Tsukihara, M.Yao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	189.100, 210.500, 178.600, 90.00, 90.00, 90.00
R / Rfree (%)	20.9 / 24.4

The structure of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Iron	(Fe)	4 atoms
Copper	(Cu)	6 atoms
Sodium	(Na)	2 atoms

The binding sites of Zinc atom in the Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State (pdb code 2occ). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State, PDB code: 2occ:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn99 b:33.1 occ:1.00 SG F:CYS82 2.2 32.1 1.0 SG F:CYS60 2.2 23.7 1.0 SG F:CYS85 2.2 37.3 1.0 SG F:CYS62 2.3 27.1 1.0 CB F:CYS82 3.2 34.1 1.0 CB F:CYS60 3.2 20.0 1.0 CB F:CYS62 3.5 23.0 1.0 CB F:CYS85 3.6 31.9 1.0 CA F:CYS62 3.7 31.2 1.0 N F:CYS85 3.9 33.2 1.0 N F:CYS62 4.0 27.5 1.0 O F:CYS60 4.2 23.8 1.0 CA F:CYS85 4.3 32.8 1.0 C F:CYS60 4.4 20.2 1.0 CA F:CYS60 4.4 17.9 1.0 CA F:CYS82 4.6 32.1 1.0 CB F:THR87 4.8 27.6 1.0 C F:ILE61 4.8 26.6 1.0 CB F:SER84 4.8 36.6 1.0 OG1 F:THR87 4.9 31.9 1.0 C F:SER84 4.9 33.3 1.0 OH F:TYR89 4.9 24.8 1.0 OG F:SER84 4.9 39.8 1.0 C F:CYS85 5.0 32.6 1.0 C F:CYS62 5.0 40.4 1.0

Zinc binding site 2 out of 2 in the Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bovine Heart Cytochrome C Oxidase at the Fully Oxidized State within 5.0Å range: probe atom residue distance (Å) B Occ S:Zn99 b:37.6 occ:1.00 SG S:CYS85 2.2 37.8 1.0 SG S:CYS60 2.2 31.6 1.0 SG S:CYS82 2.2 38.7 1.0 SG S:CYS62 2.3 34.3 1.0 CB S:CYS82 3.1 40.6 1.0 CB S:CYS60 3.3 26.9 1.0 CB S:CYS62 3.5 29.1 1.0 CB S:CYS85 3.5 38.2 1.0 CA S:CYS62 3.7 36.6 1.0 N S:CYS85 3.8 39.7 1.0 N S:CYS62 4.0 35.8 1.0 CA S:CYS85 4.2 39.1 1.0 O S:CYS60 4.4 29.7 1.0 C S:CYS60 4.4 30.3 1.0 CA S:CYS60 4.5 28.3 1.0 CA S:CYS82 4.6 36.8 1.0 CB S:SER84 4.7 38.9 1.0 C S:SER84 4.8 40.5 1.0 C S:ILE61 4.8 33.1 1.0 CB S:THR87 4.9 35.9 1.0 OG S:SER84 4.9 44.4 1.0 OG1 S:THR87 4.9 44.3 1.0 C S:CYS85 4.9 38.3 1.0 CB S:ILE70 5.0 27.0 1.0 OH S:TYR89 5.0 38.3 1.0

S.Yoshikawa, K.Shinzawa-Itoh, R.Nakashima, R.Yaono, E.Yamashita, N.Inoue, M.Yao, M.J.Fei, C.P.Libeu, T.Mizushima, H.Yamaguchi, T.Tomizaki, T.Tsukihara. Redox-Coupled Crystal Structural Changes in Bovine Heart Cytochrome C Oxidase. Science V. 280 1723 1998.
ISSN: ISSN 0036-8075
PubMed: 9624044
DOI: 10.1126/SCIENCE.280.5370.1723