Zinc in PDB 2j9a: Bllap in Complex with Microginin FR1

All present enzymatic activity of Bllap in Complex with Microginin FR1:
3.4.11.1; 3.4.11.5;

The structure of Bllap in Complex with Microginin FR1, PDB code: 2j9a was solved by M.Kraft, C.Schleberger, J.Weckesser, G.E.Schulz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.00 / 1.73
Space group	P 63 2 2
Cell size a, b, c (Å), α, β, γ (°)	129.982, 129.982, 120.813, 90.00, 90.00, 120.00
R / Rfree (%)	14.5 / 17.3

The structure of Bllap in Complex with Microginin FR1 also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Zinc atom in the Bllap in Complex with Microginin FR1 (pdb code 2j9a). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bllap in Complex with Microginin FR1, PDB code: 2j9a:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bllap in Complex with Microginin FR1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bllap in Complex with Microginin FR1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1493 b:10.7 occ:1.00 OD1 A:ASP332 1.9 8.9 1.0 OH2 D:AHY1 2.0 9.2 1.0 OE1 A:GLU334 2.0 10.8 1.0 OD2 A:ASP255 2.0 9.5 1.0 O A:ASP332 2.2 10.3 1.0 CD A:GLU334 2.9 10.1 1.0 CG A:ASP255 2.9 8.9 1.0 CG A:ASP332 3.0 10.8 1.0 C2 D:AHY1 3.1 8.1 1.0 C A:ASP332 3.1 10.5 1.0 OE2 A:GLU334 3.1 9.6 1.0 O D:AHY1 3.1 10.2 1.0 ZN A:ZN1494 3.2 11.4 1.0 OD1 A:ASP255 3.2 9.8 1.0 C1 D:AHY1 3.2 10.0 1.0 CA A:ASP332 3.4 11.6 1.0 C3 D:AHY1 3.7 10.2 1.0 CB A:ASP332 3.8 10.2 1.0 O D:HOH2001 3.9 11.8 1.0 OD2 A:ASP332 4.0 9.8 1.0 NZ A:LYS262 4.0 11.8 1.0 CE A:LYS262 4.1 11.1 1.0 N D:ALA2 4.1 10.5 1.0 N3 D:AHY1 4.1 9.2 1.0 N A:ALA333 4.3 9.3 1.0 N A:GLU334 4.3 10.1 1.0 CB A:ASP255 4.3 7.6 1.0 CG A:GLU334 4.3 11.2 1.0 ND2 A:ASN305 4.4 10.7 1.0 OD2 A:ASP273 4.7 8.4 1.0 CA A:GLY257 4.7 9.8 1.0 CA A:ALA333 4.8 10.4 1.0 N A:ASP332 4.8 9.0 1.0 NZ A:LYS250 4.9 8.8 1.0 CA D:ALA2 4.9 13.2 1.0 CB A:GLU334 4.9 10.4 1.0

Zinc binding site 2 out of 2 in the Bllap in Complex with Microginin FR1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bllap in Complex with Microginin FR1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1494 b:11.4 occ:1.00 OD2 A:ASP273 2.0 8.4 1.0 OE2 A:GLU334 2.1 9.6 1.0 NZ A:LYS250 2.1 8.8 1.0 OH2 D:AHY1 2.2 9.2 1.0 N3 D:AHY1 2.2 9.2 1.0 OD2 A:ASP255 2.5 9.5 1.0 CG A:ASP273 2.8 10.1 1.0 OD1 A:ASP273 2.9 12.3 1.0 C2 D:AHY1 2.9 8.1 1.0 CE A:LYS250 3.0 7.9 1.0 C3 D:AHY1 3.1 10.2 1.0 ZN A:ZN1493 3.2 10.7 1.0 CD A:GLU334 3.2 10.1 1.0 CG A:ASP255 3.4 8.9 1.0 O D:HOH2001 3.4 11.8 1.0 OE1 A:GLU334 3.6 10.8 1.0 CB A:ASP255 3.8 7.6 1.0 O A:THR359 4.2 11.4 1.0 CB A:ASP273 4.2 8.3 1.0 C1 D:AHY1 4.3 10.0 1.0 OD1 A:ASP255 4.4 9.8 1.0 C4 D:AHY1 4.4 8.9 1.0 CD A:LYS250 4.5 8.5 1.0 CG A:GLU334 4.5 11.2 1.0 CG2 A:ILE252 4.8 9.7 1.0 O A:ASP332 4.8 10.3 1.0 CB A:ILE252 4.8 10.2 1.0 O D:AHY1 4.8 10.2 1.0 CG1 A:ILE252 4.8 9.5 1.0 OD1 A:ASP332 4.9 8.9 1.0 N A:GLY335 4.9 10.6 1.0

M.Kraft, C.Schleberger, J.Weckesser, G.E.Schulz. Binding Structure of the Leucine Aminopeptidase Inhibitor Microginin FR1. Febs Lett. V. 580 6943 2006.
ISSN: ISSN 0014-5793
PubMed: 17157838
DOI: 10.1016/J.FEBSLET.2006.11.060