Zinc in PDB 2iuc: Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5

All present enzymatic activity of Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5:
3.1.3.1;

The structure of Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5, PDB code: 2iuc was solved by E.Wang, D.Koutsioulis, H.K.S.Leiros, O.A.Andersen, V.Bouriotis, E.Hough, P.Heikinheimo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.74 / 1.95
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	70.043, 173.184, 55.340, 90.00, 90.00, 90.00
R / Rfree (%)	16.2 / 22.5

The structure of Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5 also contains other interesting chemical elements:

Magnesium	(Mg)	6 atoms
Arsenic	(As)	1 atom

The binding sites of Zinc atom in the Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5 (pdb code 2iuc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5, PDB code: 2iuc:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1001 b:28.7 occ:1.00 NE2 A:HIS337 2.1 9.4 1.0 NE2 A:HIS263 2.1 23.5 1.0 OD1 A:ASP259 2.3 13.2 1.0 OD2 A:ASP259 2.4 18.5 1.0 CG A:ASP259 2.7 15.8 1.0 CD2 A:HIS263 3.0 22.8 1.0 CD2 A:HIS337 3.0 10.7 1.0 CE1 A:HIS337 3.1 11.9 1.0 CE1 A:HIS263 3.1 18.3 1.0 P A:PO41006 3.2 24.5 1.0 O A:HOH2176 4.1 15.8 1.0 CE1 A:HIS302 4.1 11.4 1.0 CG A:HIS263 4.1 22.3 1.0 ND1 A:HIS337 4.2 13.4 1.0 ND1 A:HIS263 4.2 27.5 1.0 CG A:HIS337 4.2 14.0 1.0 O B:HOH2077 4.2 36.8 1.0 CB A:ASP259 4.2 10.4 1.0 OG1 A:THR304 4.2 16.7 1.0 NE2 A:HIS302 4.4 10.0 1.0 ZN A:ZN1002 4.4 18.8 1.0 O B:HOH2160 4.5 29.6 1.0 OG A:SER84 4.6 19.9 1.0 OD1 A:ASP43 4.7 16.1 1.0 CG2 A:THR304 4.7 14.2 1.0 O A:ASP259 4.9 11.2 1.0

Zinc binding site 2 out of 4 in the Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1002 b:18.8 occ:1.00 OG A:SER84 2.0 19.9 1.0 OD1 A:ASP43 2.1 16.1 1.0 OD2 A:ASP301 2.1 22.2 1.0 NE2 A:HIS302 2.1 10.0 1.0 CG A:ASP43 2.7 13.7 1.0 OD2 A:ASP43 2.7 14.5 1.0 CG A:ASP301 2.9 15.4 1.0 OD1 A:ASP301 3.0 13.6 1.0 CE1 A:HIS302 3.0 11.4 1.0 CD2 A:HIS302 3.1 12.3 1.0 CB A:SER84 3.2 11.5 1.0 P A:PO41006 3.3 24.5 1.0 CA A:SER84 3.5 12.7 1.0 N A:SER84 3.9 12.2 1.0 ND1 A:HIS302 4.1 12.3 1.0 OD1 A:ASP259 4.1 13.2 1.0 CG A:HIS302 4.2 7.9 1.0 CB A:ASP43 4.2 14.3 1.0 CE1 A:HIS337 4.3 11.9 1.0 O A:HOH2083 4.3 16.3 1.0 N A:GLY44 4.3 14.3 1.0 CB A:ASP301 4.3 14.8 1.0 CG A:ASP259 4.4 15.8 1.0 ZN A:ZN1001 4.4 28.7 1.0 NE2 A:HIS337 4.4 9.4 1.0 O A:HOH2081 4.4 18.1 1.0 MG A:MG1003 4.6 15.8 1.0 CA A:ASP43 4.6 14.1 1.0 C A:ASP43 4.7 13.6 1.0 O A:HOH2176 4.7 15.8 1.0 C A:ASP83 4.7 11.8 1.0 CB A:ASP259 4.7 10.4 1.0 OD2 A:ASP259 4.8 18.5 1.0 C A:SER84 4.9 11.9 1.0 OG1 A:THR137 4.9 11.7 1.0 CA A:GLY44 5.0 14.6 1.0 O A:HOH2173 5.0 15.1 1.0

Zinc binding site 3 out of 4 in the Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1006 b:25.1 occ:1.00 NE2 B:HIS263 2.0 15.2 1.0 NE2 B:HIS337 2.1 15.0 1.0 OD2 B:ASP259 2.3 17.7 1.0 OD1 B:ASP259 2.4 17.6 1.0 CG B:ASP259 2.7 16.8 1.0 CD2 B:HIS263 2.9 17.9 1.0 CD2 B:HIS337 3.0 13.9 1.0 CE1 B:HIS263 3.0 19.0 1.0 CE1 B:HIS337 3.1 15.5 1.0 P B:PO41757 3.1 28.3 1.0 CE1 B:HIS302 4.0 9.0 1.0 CG B:HIS263 4.1 19.2 1.0 ND1 B:HIS263 4.1 21.4 1.0 O B:HOH2128 4.2 25.0 1.0 ND1 B:HIS337 4.2 10.8 1.0 CG B:HIS337 4.2 13.0 1.0 CB B:ASP259 4.2 14.9 1.0 NE2 B:HIS302 4.3 9.6 1.0 CG2 B:THR304 4.3 7.3 1.0 O A:HOH2214 4.4 30.3 1.0 ZN B:ZN1007 4.4 18.9 1.0 OG B:SER84 4.5 18.2 1.0 OD1 B:ASP43 4.7 14.1 1.0 OG1 B:THR304 4.8 16.8 1.0 CB B:THR304 4.9 17.0 1.0 O B:ASP259 4.9 13.7 1.0

Zinc binding site 4 out of 4 in the Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1007 b:18.9 occ:1.00 OD1 B:ASP43 1.9 14.1 1.0 NE2 B:HIS302 2.0 9.6 1.0 OD2 B:ASP301 2.1 16.1 1.0 OG B:SER84 2.1 18.2 1.0 CG B:ASP43 2.6 14.2 1.0 OD2 B:ASP43 2.8 15.3 1.0 CD2 B:HIS302 2.9 11.5 1.0 CG B:ASP301 3.0 15.9 1.0 CE1 B:HIS302 3.0 9.0 1.0 CB B:SER84 3.1 13.1 1.0 OD1 B:ASP301 3.3 15.6 1.0 P B:PO41757 3.4 28.3 1.0 CA B:SER84 3.5 12.2 1.0 N B:SER84 3.9 10.8 1.0 CG B:HIS302 4.0 11.5 1.0 ND1 B:HIS302 4.1 10.6 1.0 CB B:ASP43 4.1 13.8 1.0 OD1 B:ASP259 4.1 17.6 1.0 O B:HOH2062 4.2 22.9 1.0 CE1 B:HIS337 4.3 15.5 1.0 CG B:ASP259 4.3 16.8 1.0 N B:GLY44 4.3 14.9 1.0 CB B:ASP301 4.4 12.2 1.0 ZN B:ZN1006 4.4 25.1 1.0 O B:HOH2060 4.4 25.0 1.0 NE2 B:HIS337 4.5 15.0 1.0 CA B:ASP43 4.5 13.7 1.0 OD2 B:ASP259 4.6 17.7 1.0 O B:HOH2128 4.6 25.0 1.0 C B:ASP43 4.7 14.6 1.0 C B:ASP83 4.7 11.1 1.0 MG B:MG1008 4.8 19.0 1.0 CB B:ASP259 4.8 14.9 1.0 C B:SER84 4.9 12.9 1.0 OG1 B:THR137 5.0 16.3 1.0 ND1 B:HIS337 5.0 10.8 1.0

E.Wang, D.Koutsioulis, H.K.S.Leiros, O.A.Andersen, V.Bouriotis, E.Hough, P.Heikinheimo. Crystal Structure of Alkaline Phosphatase From the Antarctic Bacterium TAB5. J.Mol.Biol. V. 366 1318 2007.
ISSN: ISSN 0022-2836
PubMed: 17198711
DOI: 10.1016/J.JMB.2006.11.079