Zinc in PDB 2huc: Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants

All present enzymatic activity of Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants:
3.1.4.3;

The structure of Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants, PDB code: 2huc was solved by A.P.Benfield, S.F.Martin, N.M.Antikainen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.90
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	89.973, 89.973, 71.957, 90.00, 90.00, 90.00
R / Rfree (%)	18.7 / 20.8

The binding sites of Zinc atom in the Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants (pdb code 2huc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants, PDB code: 2huc:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn246 b:15.4 occ:1.00 O A:HOH249 2.1 12.6 1.0 ND1 A:HIS69 2.1 12.8 1.0 OD2 A:ASP122 2.2 11.9 1.0 NE2 A:HIS118 2.2 9.6 1.0 OD1 A:ASP55 2.4 10.8 1.0 CE1 A:HIS69 2.9 13.3 1.0 CG A:ASP122 3.2 12.8 1.0 CE1 A:HIS118 3.2 11.1 1.0 CD2 A:HIS118 3.2 11.7 1.0 CG A:ASP55 3.2 13.8 1.0 CG A:HIS69 3.3 12.2 1.0 ZN A:ZN248 3.3 15.5 1.0 OD2 A:ASP55 3.4 12.9 1.0 OD1 A:ASP122 3.4 11.4 1.0 O A:HOH442 3.6 50.9 1.0 CB A:HIS69 3.8 12.1 1.0 NE2 A:HIS14 4.0 10.9 1.0 NE2 A:HIS69 4.1 12.0 1.0 ND1 A:HIS118 4.3 11.0 1.0 CD2 A:HIS69 4.3 11.0 1.0 CG A:HIS118 4.3 11.6 1.0 O A:HOH250 4.4 18.2 1.0 CB A:ASP122 4.5 11.3 1.0 CE1 A:HIS14 4.6 10.9 1.0 CB A:ASP55 4.7 13.0 1.0 CE1 A:HIS128 4.7 13.9 1.0 CD2 A:HIS14 4.8 10.4 1.0 O A:TRP1 4.9 11.9 1.0 CA A:HIS69 5.0 11.6 1.0

Zinc binding site 2 out of 3 in the Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn247 b:18.8 occ:1.00 NE2 A:HIS128 2.2 11.8 1.0 NE2 A:HIS142 2.2 15.3 1.0 O A:HOH250 2.3 18.2 1.0 OE2 A:GLU146 2.4 12.0 1.0 O A:HOH251 2.4 23.9 1.0 OE1 A:GLU146 2.5 16.4 1.0 CD A:GLU146 2.7 15.6 1.0 CE1 A:HIS128 3.1 13.9 1.0 CE1 A:HIS142 3.2 15.2 1.0 CD2 A:HIS128 3.2 13.1 1.0 CD2 A:HIS142 3.2 15.0 1.0 O A:HOH442 4.1 50.9 1.0 N A:TRP1 4.1 10.3 1.0 CG A:GLU146 4.1 14.7 1.0 ND1 A:HIS128 4.2 12.8 1.0 O A:HOH338 4.2 27.1 1.0 ND1 A:HIS142 4.3 15.1 1.0 CG A:HIS128 4.3 13.2 1.0 CG A:HIS142 4.4 16.1 1.0 O A:HOH259 4.5 23.8 1.0 OE1 A:GLN125 4.5 16.9 1.0 O A:HOH249 4.5 12.6 1.0 ZN A:ZN248 4.7 15.5 1.0 O A:HOH256 4.9 35.4 1.0 CA A:TRP1 4.9 12.4 1.0 O A:TRP1 5.0 11.9 1.0 O A:HOH254 5.0 34.4 1.0

Zinc binding site 3 out of 3 in the Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structural Studies Examining the Substrate Specificity Profiles of Pc- Plcbc Protein Variants within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn248 b:15.5 occ:1.00 O A:HOH249 2.1 12.6 1.0 NE2 A:HIS14 2.1 10.9 1.0 OD1 A:ASP122 2.2 11.4 1.0 N A:TRP1 2.2 10.3 1.0 O A:TRP1 2.3 11.9 1.0 C A:TRP1 3.0 12.4 1.0 CD2 A:HIS14 3.0 10.4 1.0 O A:HOH250 3.0 18.2 1.0 CA A:TRP1 3.0 12.4 1.0 CG A:ASP122 3.2 12.8 1.0 CE1 A:HIS14 3.2 10.9 1.0 ZN A:ZN246 3.3 15.4 1.0 OD2 A:ASP122 3.5 11.9 1.0 CB A:TRP1 3.7 11.0 1.0 OD2 A:ASP55 4.0 12.9 1.0 NE2 A:HIS118 4.0 9.6 1.0 CG A:HIS14 4.2 10.1 1.0 ND1 A:HIS14 4.2 10.9 1.0 N A:SER2 4.3 13.4 1.0 CE1 A:HIS118 4.3 11.1 1.0 CG A:TRP1 4.4 14.1 1.0 CD1 A:TRP1 4.5 12.4 1.0 CE1 A:HIS128 4.5 13.9 1.0 CB A:ASP122 4.5 11.3 1.0 NE2 A:HIS128 4.6 11.8 1.0 OE2 A:GLU146 4.6 12.0 1.0 ZN A:ZN247 4.7 18.8 1.0 OD1 A:ASP55 4.7 10.8 1.0 CA A:ASP122 4.8 10.5 1.0 CG A:ASP55 4.8 13.8 1.0 O A:HOH442 4.8 50.9 1.0 O A:HOH338 4.9 27.1 1.0 CD2 A:HIS118 4.9 11.7 1.0 O A:SER2 5.0 10.7 1.0 CA A:SER2 5.0 13.4 1.0

A.P.Benfield, N.M.Goodey, L.T.Phillips, S.F.Martin. Structural Studies Examining the Substrate Specificity Profiles of Pc-Plc(Bc) Protein Variants. Arch.Biochem.Biophys. V. 460 41 2007.
ISSN: ISSN 0003-9861
PubMed: 17324372
DOI: 10.1016/J.ABB.2007.01.023