Zinc in PDB 2glq: X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium

All present enzymatic activity of X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium:
3.1.3.1;

The structure of X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium, PDB code: 2glq was solved by P.Llinas, M.Masella, T.Stigbrand, A.Menez, E.A.Stura, M.H.Le Du, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	9.99 / 1.60
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	89.090, 115.299, 107.320, 90.00, 90.00, 90.00
R / Rfree (%)	14.8 / 18.8

The structure of X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium also contains other interesting chemical elements:

Strontium	(Sr)	1 atom
Magnesium	(Mg)	1 atom

The binding sites of Zinc atom in the X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium (pdb code 2glq). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium, PDB code: 2glq:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2001 b:12.7 occ:1.00 CE1 A:HIS432 2.1 10.4 1.0 O A:HOH2579 2.1 18.9 1.0 OD1 A:ASP316 2.1 13.0 1.0 O1P A:SEP92 2.2 11.3 1.0 CE1 A:HIS320 2.2 22.8 1.0 OD2 A:ASP316 2.3 11.2 1.0 CG A:ASP316 2.6 11.9 1.0 NE2 A:HIS320 2.7 20.7 1.0 NE2 A:HIS432 2.9 13.7 1.0 ND1 A:HIS432 3.2 13.3 1.0 P A:SEP92 3.2 14.3 1.0 ND1 A:HIS320 3.3 26.0 1.0 O2P A:SEP92 3.7 13.0 1.0 O A:HOH2338 3.7 33.3 1.0 O A:HOH2222 3.8 36.0 1.0 CD2 A:HIS320 3.9 23.0 1.0 O3P A:SEP92 3.9 11.8 1.0 O A:HOH2015 4.0 11.7 1.0 NE2 A:HIS358 4.0 15.5 1.0 ZN A:ZN2002 4.0 11.9 1.0 CB A:ASP316 4.1 12.1 1.0 CD2 A:HIS432 4.1 8.3 1.0 CG A:HIS320 4.2 17.3 1.0 CG A:HIS432 4.3 12.0 1.0 CE1 A:HIS358 4.4 12.5 1.0 CE1 A:HIS360 4.4 13.5 1.0 OG A:SEP92 4.4 14.6 1.0 NE2 A:HIS360 4.5 12.6 1.0 OD1 A:ASP42 4.5 10.5 1.0 O A:ASP316 4.9 11.2 1.0

Zinc binding site 2 out of 2 in the X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of X-Ray Structure of Human Alkaline Phosphatase in Complex with Strontium within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2002 b:11.9 occ:1.00 OD1 A:ASP42 1.9 10.5 1.0 OD2 A:ASP357 2.0 10.2 1.0 CE1 A:HIS358 2.0 12.5 1.0 O1P A:SEP92 2.1 11.3 1.0 OG A:SEP92 2.2 14.6 1.0 CG A:ASP42 2.7 12.0 1.0 OD2 A:ASP42 2.9 10.9 1.0 CG A:ASP357 2.9 13.0 1.0 P A:SEP92 3.0 14.3 1.0 ND1 A:HIS358 3.0 15.8 1.0 NE2 A:HIS358 3.0 15.5 1.0 OD1 A:ASP357 3.1 13.6 1.0 CB A:SEP92 3.4 14.5 1.0 OD2 A:ASP316 3.8 11.2 1.0 O2P A:SEP92 3.9 13.0 1.0 CA A:SEP92 4.0 13.4 1.0 O3P A:SEP92 4.0 11.8 1.0 CG A:ASP316 4.0 11.9 1.0 ZN A:ZN2001 4.0 12.7 1.0 NE2 A:HIS432 4.1 13.7 1.0 CB A:ASP42 4.1 11.6 1.0 CD2 A:HIS358 4.1 12.4 1.0 CG A:HIS358 4.1 11.2 1.0 CE1 A:HIS432 4.3 10.4 1.0 N A:SEP92 4.3 13.5 1.0 N A:GLY43 4.3 11.8 1.0 OD1 A:ASP316 4.3 13.0 1.0 CB A:ASP357 4.3 12.3 1.0 O A:HOH2039 4.4 12.2 1.0 CA A:ASP42 4.5 11.2 1.0 C A:ASP42 4.5 11.6 1.0 CB A:ASP316 4.6 12.1 1.0 O A:HOH2015 4.7 11.7 1.0 O A:HOH2578 4.7 9.6 1.0 MG A:MG2003 4.7 12.6 1.0 CD2 A:HIS432 4.9 8.3 1.0 CA A:GLY43 5.0 10.8 1.0

P.Llinas, M.Masella, T.Stigbrand, A.Menez, E.A.Stura, M.H.Le Du. Structural Studies of Human Alkaline Phosphatase in Complex with Strontium: Implication For Its Secondary Effect in Bones. Protein Sci. V. 15 1691 2006.
ISSN: ISSN 0961-8368
PubMed: 16815919
DOI: 10.1110/PS.062123806