Zinc in PDB 2gc3: The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc

All present enzymatic activity of The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc:
5.3.1.9;

The structure of The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc, PDB code: 2gc3 was solved by J.M.Berrisford, D.W.Rice, P.J.Baker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.85 / 2.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	70.346, 74.016, 74.835, 90.00, 90.00, 90.00
R / Rfree (%)	15.8 / 23.5

The binding sites of Zinc atom in the The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc (pdb code 2gc3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc, PDB code: 2gc3:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn402 b:18.6 occ:1.00 OE2 A:GLU97 1.9 17.7 1.0 NE2 A:HIS136 2.2 17.7 1.0 O A:HOH676 2.2 10.5 1.0 NE2 A:HIS90 2.2 15.4 1.0 NE2 A:HIS88 2.3 19.2 1.0 CD A:GLU97 3.0 25.3 1.0 CD2 A:HIS90 3.0 11.2 1.0 CE1 A:HIS136 3.1 9.7 1.0 CD2 A:HIS136 3.2 11.7 1.0 CE1 A:HIS88 3.2 12.9 1.0 CD2 A:HIS88 3.3 11.0 1.0 CE1 A:HIS90 3.3 21.2 1.0 OE1 A:GLU97 3.5 24.1 1.0 O2 A:M6P501 3.7 19.9 0.8 O A:HOH510 4.2 16.1 1.0 ND1 A:HIS136 4.2 16.7 1.0 CG A:HIS90 4.2 21.1 1.0 ND1 A:HIS90 4.3 10.1 1.0 CG A:GLU97 4.3 15.3 1.0 CG A:HIS136 4.3 14.7 1.0 ND1 A:HIS88 4.3 13.8 1.0 O3 A:M6P501 4.4 14.9 0.8 CG A:HIS88 4.4 16.0 1.0 CB A:GLU97 4.6 10.2 1.0 OH A:TYR99 4.6 16.6 1.0 C2 A:M6P501 4.9 16.7 0.8

Zinc binding site 2 out of 2 in the The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Crystal Structure of Phosphoglucose Isomerase From Pyrococcus Furiosus in Complex with Mannose 6-Phosphate and Zinc within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:25.9 occ:1.00 OE1 B:GLU97 2.0 27.5 1.0 NE2 B:HIS90 2.1 27.0 1.0 O B:HOH669 2.1 18.1 1.0 NE2 B:HIS136 2.3 25.6 1.0 NE2 B:HIS88 2.4 39.5 1.0 CD2 B:HIS90 3.1 15.1 1.0 CE1 B:HIS90 3.1 27.1 1.0 CE1 B:HIS136 3.1 26.1 1.0 CD B:GLU97 3.1 29.7 1.0 CD2 B:HIS88 3.3 36.1 1.0 CD2 B:HIS136 3.4 18.6 1.0 CE1 B:HIS88 3.4 28.4 1.0 O2 B:M6P502 3.6 23.3 0.8 OE2 B:GLU97 3.6 35.6 1.0 ND1 B:HIS90 4.1 30.9 1.0 CG B:HIS90 4.2 22.6 1.0 O B:HOH600 4.2 25.4 1.0 ND1 B:HIS136 4.3 22.6 1.0 CG B:GLU97 4.4 17.4 1.0 CG B:HIS88 4.4 34.6 1.0 CG B:HIS136 4.5 20.6 1.0 ND1 B:HIS88 4.5 42.4 1.0 O3 B:M6P502 4.5 14.2 0.8 CB B:GLU97 4.5 12.8 1.0 OH B:TYR99 4.6 24.2 1.0 NE2 B:HIS158 4.9 30.3 1.0 C2 B:M6P502 5.0 19.6 0.8 CD2 B:HIS158 5.0 38.0 1.0 CB B:TYR152 5.0 10.3 1.0

J.M.Berrisford, A.M.Hounslow, J.Akerboom, W.R.Hagen, S.J.Brouns, J.Van Der Oost, I.A.Murray, G.Michael Blackburn, J.P.Waltho, D.W.Rice, P.J.Baker. Evidence Supporting A Cis-Enediol-Based Mechanism For Pyrococcus Furiosus Phosphoglucose Isomerase J.Mol.Biol. V. 358 1353 2006.
ISSN: ISSN 0022-2836
PubMed: 16580686
DOI: 10.1016/J.JMB.2006.03.015