Zinc in PDB 2e88: Crystal Structure of the Human HSP70 Atpase Domain in the Apo Form

The structure of Crystal Structure of the Human HSP70 Atpase Domain in the Apo Form, PDB code: 2e88 was solved by M.Shida, R.Ishii, T.Takagi, S.Kishishita, M.Shirouzu, S.Yokoyama, Riken Structural Genomics/Proteomics Initiative (Rsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.010, 62.120, 143.070, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 23.9

The binding sites of Zinc atom in the Crystal Structure of the Human HSP70 Atpase Domain in the Apo Form (pdb code 2e88). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of the Human HSP70 Atpase Domain in the Apo Form, PDB code: 2e88:

Zinc binding site 1 out of 1 in the Crystal Structure of the Human HSP70 Atpase Domain in the Apo Form


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Human HSP70 Atpase Domain in the Apo Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:22.7 occ:1.00 ND1 A:HIS227 2.1 22.6 1.0 OD1 A:ASP225 2.1 18.8 1.0 CG A:ASP225 2.9 24.0 1.0 CE1 A:HIS227 3.0 32.2 1.0 CG A:HIS227 3.1 21.6 1.0 O A:HOH734 3.2 27.7 1.0 OD2 A:ASP225 3.2 19.1 1.0 CB A:HIS227 3.4 17.7 1.0 O A:HOH605 3.8 28.3 1.0 N A:HIS227 3.9 16.9 1.0 NE2 A:HIS227 4.2 24.9 1.0 CB A:ASP225 4.2 17.8 1.0 CD2 A:HIS227 4.2 25.2 1.0 CA A:HIS227 4.3 14.1 1.0 N A:THR226 4.4 18.8 1.0 CA A:ASP225 4.4 21.5 1.0 O A:HOH653 4.5 21.1 1.0 C A:ASP225 4.6 23.7 1.0 OG1 A:THR226 4.9 25.6 1.0 C A:THR226 5.0 23.5 1.0

M.Shida, A.Arakawa, R.Ishii, S.Kishishita, T.Takagi, M.Kukimoto-Niino, S.Sugano, A.Tanaka, M.Shirouzu, S.Yokoyama. Direct Inter-Subdomain Interactions Switch Between the Closed and Open Forms of the HSP70 Nucleotide-Binding Domain in the Nucleotide-Free State. Acta Crystallogr.,Sect.D V. 66 223 2010.
ISSN: ISSN 0907-4449
PubMed: 20179333
DOI: 10.1107/S0907444909053979