Zinc in PDB 2chi: Recombinant Human H Ferritin, K86Q and E27D Mutant

All present enzymatic activity of Recombinant Human H Ferritin, K86Q and E27D Mutant:
1.16.3.1;

The structure of Recombinant Human H Ferritin, K86Q and E27D Mutant, PDB code: 2chi was solved by L.Toussaint, R.R.Crichton, J.P.Declercq, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.08 / 1.60
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	180.528, 180.528, 180.528, 90.00, 90.00, 90.00
R / Rfree (%)	16.8 / 19.3

The structure of Recombinant Human H Ferritin, K86Q and E27D Mutant also contains other interesting chemical elements:

Calcium

(Ca)

3 atoms

The binding sites of Zinc atom in the Recombinant Human H Ferritin, K86Q and E27D Mutant (pdb code 2chi). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Recombinant Human H Ferritin, K86Q and E27D Mutant, PDB code: 2chi:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Recombinant Human H Ferritin, K86Q and E27D Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Recombinant Human H Ferritin, K86Q and E27D Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn212 b:27.7 occ:1.00 OE1 A:GLU62 2.0 20.6 1.0 OD1 A:ASP27 2.1 24.3 1.0 O A:HOH2312 2.1 23.6 1.0 O A:HOH2311 2.2 26.3 1.0 ND1 A:HIS65 2.2 21.4 1.0 CD A:GLU62 3.0 21.6 1.0 CE1 A:HIS65 3.1 23.5 1.0 CG A:ASP27 3.1 20.6 1.0 CG A:HIS65 3.3 18.6 1.0 OE2 A:GLU62 3.4 24.6 1.0 CB A:HIS65 3.6 16.0 1.0 OD2 A:ASP27 3.7 23.6 1.0 O A:HOH2262 3.9 30.1 1.0 CG1 A:VAL110 4.2 18.0 1.0 OE1 A:GLN141 4.2 21.4 1.0 CG A:GLU62 4.3 17.7 1.0 NE2 A:HIS65 4.3 24.3 1.0 CB A:ASP27 4.3 16.1 1.0 CA A:GLU62 4.4 15.5 1.0 CD2 A:HIS65 4.4 21.4 1.0 O A:HOH2083 4.4 61.3 1.0 CB A:GLU62 4.5 14.8 1.0 O A:HOH2261 4.6 25.6 1.0 CA A:ASP27 4.8 15.6 1.0 O A:GLU62 4.9 15.8 1.0

Zinc binding site 2 out of 2 in the Recombinant Human H Ferritin, K86Q and E27D Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Recombinant Human H Ferritin, K86Q and E27D Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn214 b:41.8 occ:0.25 O A:HOH2313 2.1 12.8 0.2 NE2 A:HIS173 2.4 30.9 1.0 CE1 A:HIS173 2.6 27.6 1.0 CD2 A:HIS173 3.8 26.4 1.0 ND1 A:HIS173 3.9 28.2 1.0 CG A:HIS173 4.5 21.4 1.0 CD2 A:LEU169 5.0 14.2 1.0

L.Toussaint, L.Bertrand, L.Hue, R.R.Crichton, J.P.Declercq. High-Resolution X-Ray Structures of Human Apoferritin H-Chain Mutants Correlated with Their Activity and Metal-Binding Sites. J.Mol.Biol. V. 365 440 2007.
ISSN: ISSN 0022-2836
PubMed: 17070541
DOI: 10.1016/J.JMB.2006.10.010