Zinc in PDB 2c6g: Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate

All present enzymatic activity of Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate:
3.4.17.21;

The structure of Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate, PDB code: 2c6g was solved by J.R.Mesters, C.Barinka, W.Li, T.Tsukamoto, P.Majer, B.S.Slusher, J.Konvalinka, R.Hilgenfeld, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	100.00 / 2.20
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	102.435, 130.428, 159.775, 90.00, 90.00, 90.00
R / Rfree (%)	18.6 / 22.8

The structure of Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Calcium	(Ca)	1 atom

The binding sites of Zinc atom in the Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate (pdb code 2c6g). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate, PDB code: 2c6g:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1751 b:27.1 occ:1.00 OE2 A:GLU425 1.9 20.3 1.0 OD2 A:ASP387 1.9 24.3 1.0 NE2 A:HIS553 2.2 30.0 1.0 O A:HOH2139 2.3 25.4 1.0 OE1 A:GLU425 2.5 23.7 1.0 CD A:GLU425 2.5 19.9 1.0 CG A:ASP387 3.0 29.4 1.0 CE1 A:HIS553 3.1 30.5 1.0 O A:HOH2141 3.1 43.0 1.0 CD2 A:HIS553 3.2 25.0 1.0 ZN A:ZN1752 3.3 25.3 1.0 OD1 A:ASP387 3.4 31.4 1.0 OE1 A:GLU424 4.0 25.1 1.0 CG A:GLU425 4.0 21.1 1.0 CA A:GLU1766 4.1 27.9 1.0 N A:GLU1766 4.1 26.2 1.0 CE1 A:TYR552 4.1 29.5 1.0 O A:HOH2048 4.2 26.4 1.0 ND1 A:HIS553 4.2 30.0 1.0 CG A:HIS553 4.3 31.3 1.0 CB A:ASP387 4.3 27.6 1.0 OH A:TYR552 4.3 28.5 1.0 NE2 A:HIS377 4.4 22.2 1.0 C A:GLU1766 4.5 28.4 1.0 CE1 A:HIS377 4.6 26.8 1.0 CZ A:TYR552 4.6 28.9 1.0 CD1 A:TRP381 4.6 26.9 1.0 OXT A:GLU1766 4.7 28.9 1.0 NE1 A:TRP381 4.7 26.5 1.0 CD A:GLU424 5.0 26.1 1.0

Zinc binding site 2 out of 2 in the Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Membrane-Bound Glutamate Carboxypeptidase II (Gcpii) with Bound Glutamate within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1752 b:25.3 occ:1.00 O A:HOH2139 1.8 25.4 1.0 NE2 A:HIS377 1.9 22.2 1.0 OD1 A:ASP387 2.0 31.4 1.0 OD2 A:ASP453 2.1 23.9 1.0 OD1 A:ASP453 2.7 22.4 1.0 CG A:ASP453 2.8 26.1 1.0 CE1 A:HIS377 2.9 26.8 1.0 CG A:ASP387 2.9 29.4 1.0 CD2 A:HIS377 3.0 24.4 1.0 OD2 A:ASP387 3.1 24.3 1.0 ZN A:ZN1751 3.3 27.1 1.0 OE1 A:GLU424 3.5 25.1 1.0 OE2 A:GLU425 3.6 20.3 1.0 O A:HOH2141 3.9 43.0 1.0 ND1 A:HIS377 4.0 20.6 1.0 CD A:GLU424 4.0 26.1 1.0 CG A:HIS377 4.1 24.1 1.0 CB A:PRO388 4.2 26.2 1.0 ND2 A:ASN519 4.2 23.6 1.0 CB A:ASP453 4.3 25.1 1.0 CB A:ASP387 4.3 27.6 1.0 CD A:GLU425 4.4 19.9 1.0 OE2 A:GLU424 4.5 25.2 1.0 CA A:PRO388 4.5 26.8 1.0 N A:PRO388 4.6 25.9 1.0 CA A:ASP387 4.6 27.9 1.0 C A:ASP387 4.6 27.7 1.0 N A:GLU1766 4.8 26.2 1.0 OG A:SER454 4.8 26.6 1.0 OE1 A:GLU425 4.8 23.7 1.0 CG A:GLU424 5.0 21.5 1.0

J.R.Mesters, C.Barinka, W.Li, T.Tsukamoto, P.Majer, B.S.Slusher, J.Konvalinka, R.Hilgenfeld. Structure of Glutamate Carboxypeptidase II, A Drug Target in Neuronal Damage and Prostate Cancer. Embo J. V. 25 1375 2006.
ISSN: ISSN 0261-4189
PubMed: 16467855
DOI: 10.1038/SJ.EMBOJ.7600969