Atomistry » Zinc » PDB 1zs0-2a21 » 2a0f
Atomistry »
  Zinc »
    PDB 1zs0-2a21 »
      2a0f »

Zinc in PDB 2a0f: Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution

Enzymatic activity of Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution

All present enzymatic activity of Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution:
2.1.3.2;

Protein crystallography data

The structure of Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution, PDB code: 2a0f was solved by K.A.Stieglitz, K.J.Dusinberre, J.P.Cardia, H.Tsuruta, E.R.Kantrowitz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 2.90
Space group P 3 2 1
Cell size a, b, c (Å), α, β, γ (°) 120.452, 120.452, 155.242, 90.00, 90.00, 120.00
R / Rfree (%) 21.7 / 27.9

Zinc Binding Sites:

The binding sites of Zinc atom in the Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution (pdb code 2a0f). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution, PDB code: 2a0f:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 2a0f

Go back to Zinc Binding Sites List in 2a0f
Zinc binding site 1 out of 2 in the Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn154

b:45.6
occ:1.00
SG B:CYS138 2.5 46.3 1.0
CB B:CYS138 2.6 42.3 1.0
SG B:CYS114 2.6 61.8 1.0
SG B:CYS141 2.6 56.8 1.0
SG B:CYS109 2.7 81.7 1.0
O B:HOH221 3.3 81.1 1.0
CB B:CYS114 3.4 62.4 1.0
OG B:SER116 3.4 60.3 1.0
CB B:CYS109 3.9 84.4 1.0
CE2 B:PHE125 4.0 66.8 1.0
CA B:CYS138 4.1 42.2 1.0
CB B:ASN111 4.3 79.0 1.0
CB B:CYS141 4.4 55.7 1.0
CB B:SER116 4.4 62.0 1.0
CD2 B:PHE125 4.7 67.0 1.0
N B:CYS141 4.7 52.1 1.0
C B:CYS138 4.8 41.3 1.0
O B:CYS138 4.8 40.5 1.0
N B:CYS138 4.8 43.6 1.0
CA B:CYS114 4.9 63.3 1.0
O B:ASN111 4.9 73.9 1.0
CZ B:PHE125 4.9 66.4 1.0
CG B:ASN111 5.0 80.2 1.0

Zinc binding site 2 out of 2 in 2a0f

Go back to Zinc Binding Sites List in 2a0f
Zinc binding site 2 out of 2 in the Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of D236A Mutant E. Coli Aspartate Transcarbamoylase in Presence of Phosphonoacetamide at 2.90 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn154

b:47.3
occ:1.00
CB D:CYS114 2.9 0.9 1.0
OG D:SER116 3.0 0.9 1.0
SG D:CYS138 3.3 0.5 1.0
O D:HOH174 3.3 43.7 1.0
SG D:CYS114 3.4 0.8 1.0
SG D:CYS109 3.8 0.6 1.0
O D:HOH156 3.9 89.9 1.0
CB D:TYR140 4.1 96.2 1.0
CB D:SER116 4.2 0.7 1.0
ND2 D:ASN111 4.3 0.1 1.0
O D:CYS114 4.3 0.2 1.0
CA D:CYS114 4.3 0.0 1.0
CB D:CYS141 4.4 99.8 1.0
N D:CYS141 4.5 96.5 1.0
CE2 D:PHE125 4.5 0.2 1.0
SG D:CYS141 4.6 0.0 1.0
N D:TYR140 4.6 97.6 1.0
CB D:ASN111 4.7 0.2 1.0
CE1 D:HIS117 4.7 0.2 1.0
CA D:TYR140 4.7 96.9 1.0
C D:CYS114 4.7 0.3 1.0
C D:TYR140 4.8 96.6 1.0
O D:ASN111 4.8 0.5 1.0
CB D:CYS109 4.9 0.8 1.0
CB D:CYS138 5.0 0.6 1.0
CG D:TYR140 5.0 95.0 1.0

Reference:

K.A.Stieglitz, K.J.Dusinberre, J.P.Cardia, H.Tsuruta, E.R.Kantrowitz. Structure of the E.Coli Aspartate Transcarbamoylase Trapped in the Middle of the Catalytic Cycle. J.Mol.Biol. V. 352 478 2005.
ISSN: ISSN 0022-2836
PubMed: 16120448
DOI: 10.1016/J.JMB.2005.07.046
Page generated: Wed Dec 16 03:16:27 2020

Last articles

Zn in 7VD8
Zn in 7V1R
Zn in 7V1Q
Zn in 7VPF
Zn in 7T85
Zn in 7T5F
Zn in 7NF9
Zn in 7M4M
Zn in 7M4O
Zn in 7M4N
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy