Zinc in PDB 1wav: Crystal Structure of Form B Monoclinic Crystal of Insulin

The structure of Crystal Structure of Form B Monoclinic Crystal of Insulin, PDB code: 1wav was solved by D.-C.Liang, J.-H.Ding, W.-R.Chang, Z.-L.Wan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	49.240, 60.940, 48.180, 90.00, 95.80, 90.00
R / Rfree (%)	20.6 / n/a

The binding sites of Zinc atom in the Crystal Structure of Form B Monoclinic Crystal of Insulin (pdb code 1wav). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Form B Monoclinic Crystal of Insulin, PDB code: 1wav:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Form B Monoclinic Crystal of Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Form B Monoclinic Crystal of Insulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn31 b:27.2 occ:1.00 O F:HOH118 1.6 17.4 1.0 NE2 J:HIS10 2.5 26.1 1.0 NE2 B:HIS10 2.6 9.9 1.0 NE2 F:HIS10 2.8 23.8 1.0 CD2 B:HIS10 3.2 11.3 1.0 CD2 J:HIS10 3.3 20.3 1.0 CE1 J:HIS10 3.5 23.9 1.0 CD2 F:HIS10 3.5 24.7 1.0 CE1 F:HIS10 3.7 26.1 1.0 CE1 B:HIS10 3.8 12.3 1.0 O B:LEU6 4.1 13.4 1.0 O J:LEU6 4.1 9.9 1.0 CG B:LEU6 4.3 17.1 1.0 CB B:LEU6 4.4 13.3 1.0 O F:LEU6 4.4 24.1 1.0 CG B:HIS10 4.5 10.6 1.0 CG J:LEU6 4.5 17.5 1.0 CG J:HIS10 4.5 21.3 1.0 CD2 B:LEU6 4.5 20.5 1.0 ND1 J:HIS10 4.5 23.2 1.0 CG F:LEU6 4.6 10.8 1.0 ND1 B:HIS10 4.7 10.7 1.0 CG F:HIS10 4.7 19.3 1.0 ND1 F:HIS10 4.8 22.1 1.0 CB J:LEU6 4.8 18.1 1.0 C B:LEU6 4.9 12.2 1.0 O B:HOH38 4.9 50.1 1.0 C J:LEU6 4.9 11.8 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Form B Monoclinic Crystal of Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Form B Monoclinic Crystal of Insulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn31 b:29.2 occ:1.00 O L:HOH119 1.5 36.1 1.0 NE2 D:HIS10 2.6 11.5 1.0 NE2 H:HIS10 2.9 11.2 1.0 NE2 L:HIS10 2.9 17.6 1.0 CD2 D:HIS10 3.1 9.7 1.0 O D:LEU6 3.4 15.0 1.0 CE1 H:HIS10 3.6 12.0 1.0 CD2 H:HIS10 3.6 11.4 1.0 CB D:LEU6 3.6 17.1 1.0 CE1 L:HIS10 3.7 13.8 1.0 CE1 D:HIS10 3.8 11.6 1.0 CD2 L:HIS10 3.9 15.0 1.0 CD2 D:LEU6 4.0 16.7 1.0 C D:LEU6 4.0 15.3 1.0 CG D:LEU6 4.0 20.9 1.0 CA D:LEU6 4.2 15.2 1.0 CG D:HIS10 4.3 11.7 1.0 CB L:LEU6 4.5 14.8 1.0 CD1 L:LEU6 4.5 12.2 1.0 O H:LEU6 4.5 24.2 1.0 O L:HOH92 4.6 36.2 1.0 ND1 H:HIS10 4.6 11.2 1.0 ND1 D:HIS10 4.6 14.3 1.0 CG H:HIS10 4.7 10.7 1.0 N D:CYS7 4.7 19.4 1.0 ND1 L:HIS10 4.9 12.1 1.0 CG L:HIS10 5.0 10.8 1.0

J.Ding, Z.Wan, W.Chang, D.Liang. Molecular Replacement Study on Form-B Monoclinic Crystal of Insulin. Sci.China, Ser.C: Life Sci. V. 39 144 1996.
ISSN: ISSN 1006-9305
PubMed: 8760462