Atomistry » Zinc » PDB 1w56-1wfl » 1w8p
Atomistry »
  Zinc »
    PDB 1w56-1wfl »
      1w8p »

Zinc in PDB 1w8p: Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant.

Protein crystallography data

The structure of Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant., PDB code: 1w8p was solved by L.Zakowa, O.Au-Alvarez, E.J.Dodson, G.G.Dodson, A.M.Brzozowski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 25.57 / 2.08
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 59.903, 62.116, 47.796, 90.00, 110.58, 90.00
R / Rfree (%) 18.9 / 25.6

Zinc Binding Sites:

The binding sites of Zinc atom in the Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant. (pdb code 1w8p). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant., PDB code: 1w8p:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 1w8p

Go back to Zinc Binding Sites List in 1w8p
Zinc binding site 1 out of 2 in the Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant. within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1030

b:27.9
occ:1.00
NE2 B:HIS10 2.0 27.1 1.0
NE2 F:HIS10 2.1 24.0 1.0
NE2 J:HIS10 2.1 25.4 1.0
O B:HOH2009 2.2 16.6 1.0
CE1 B:HIS10 3.0 29.1 1.0
CE1 J:HIS10 3.0 30.1 1.0
CD2 B:HIS10 3.0 29.3 1.0
CE1 F:HIS10 3.0 28.2 1.0
CD2 F:HIS10 3.1 25.6 1.0
CD2 J:HIS10 3.1 25.8 1.0
ND1 B:HIS10 4.1 30.5 1.0
CG B:HIS10 4.1 28.3 1.0
ND1 J:HIS10 4.1 27.1 1.0
ND1 F:HIS10 4.1 24.6 1.0
CG F:HIS10 4.2 27.0 1.0
CG J:HIS10 4.2 27.6 1.0
O F:LEU6 4.5 28.7 1.0
O B:LEU6 4.8 24.8 1.0
CB F:LEU6 4.8 30.0 1.0
O J:LEU6 4.9 27.4 1.0
CB B:LEU6 5.0 24.6 1.0

Zinc binding site 2 out of 2 in 1w8p

Go back to Zinc Binding Sites List in 1w8p
Zinc binding site 2 out of 2 in the Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant. within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn1030

b:26.6
occ:1.00
CE1 D:HIS10 1.8 25.1 1.0
NE2 L:HIS10 2.0 20.7 1.0
NE2 H:HIS10 2.1 24.2 1.0
O D:HOH2012 2.2 17.6 1.0
NE2 D:HIS10 2.2 26.7 1.0
CE1 H:HIS10 2.9 27.5 1.0
CE1 L:HIS10 3.0 23.3 1.0
CD2 L:HIS10 3.1 24.0 1.0
ND1 D:HIS10 3.1 29.7 1.0
CD2 H:HIS10 3.2 25.9 1.0
CD2 D:HIS10 3.6 25.7 1.0
CG D:HIS10 4.0 26.9 1.0
ND1 H:HIS10 4.1 29.2 1.0
ND1 L:HIS10 4.1 22.5 1.0
CG L:HIS10 4.2 25.3 1.0
CG H:HIS10 4.2 26.6 1.0
O L:LEU6 4.6 31.9 1.0
CB L:LEU6 4.9 31.8 1.0
O D:LEU6 4.9 31.6 1.0
CB H:LEU6 4.9 26.0 1.0
O H:LEU6 4.9 27.3 1.0
O H:HOH2003 5.0 59.7 1.0

Reference:

L.Zarkowa, J.Brynda, O.Au-Alvarez, E.J.Dodson, G.G.Dodson, J.L.Whittingham, A.M.Brzozowski. Towards the Insulin-Igf-I Intermediate Structures: Functional and Structural Properties of the B25TYR-Nme-B26PHE Insulin Mutant. Biochemistry V. 43 16293 2004.
ISSN: ISSN 0006-2960
PubMed: 15610023
DOI: 10.1021/BI048856U
Page generated: Wed Dec 16 03:08:30 2020

Last articles

Zn in 7VD8
Zn in 7V1R
Zn in 7V1Q
Zn in 7VPF
Zn in 7T85
Zn in 7T5F
Zn in 7NF9
Zn in 7M4M
Zn in 7M4O
Zn in 7M4N
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy