Zinc in PDB 1uaq: The Crystal Structure of Yeast Cytosine Deaminase

All present enzymatic activity of The Crystal Structure of Yeast Cytosine Deaminase:
3.5.4.1;

The structure of The Crystal Structure of Yeast Cytosine Deaminase, PDB code: 1uaq was solved by T.-P.Ko, J.-J.Lin, C.-Y.Hu, Y.-H.Hsu, A.H.-J.Wang, S.-H.Liaw, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.79 / 1.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	47.113, 53.681, 68.076, 90.00, 105.33, 90.00
R / Rfree (%)	17.7 / 21.5

The binding sites of Zinc atom in the The Crystal Structure of Yeast Cytosine Deaminase (pdb code 1uaq). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the The Crystal Structure of Yeast Cytosine Deaminase, PDB code: 1uaq:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the The Crystal Structure of Yeast Cytosine Deaminase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Crystal Structure of Yeast Cytosine Deaminase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn200 b:17.8 occ:1.00 O4 A:DUC300 2.1 15.6 1.0 ND1 A:HIS62 2.1 14.9 1.0 SG A:CYS94 2.3 16.8 1.0 SG A:CYS91 2.3 18.2 1.0 CE1 A:HIS62 3.0 18.3 1.0 CG A:HIS62 3.1 17.0 1.0 C4 A:DUC300 3.1 18.9 1.0 CB A:CYS94 3.3 17.0 1.0 CB A:CYS91 3.3 18.2 1.0 N3 A:DUC300 3.5 17.4 1.0 CB A:HIS62 3.5 16.3 1.0 C5 A:DUC300 3.6 16.6 1.0 N A:CYS91 3.8 17.9 1.0 OE2 A:GLU64 3.8 17.9 1.0 N A:CYS94 3.9 17.7 1.0 C6 A:DUC300 4.1 16.7 1.0 C2 A:DUC300 4.1 15.7 1.0 CA A:CYS91 4.1 19.7 1.0 NE2 A:HIS62 4.1 17.4 1.0 CE A:MET93 4.2 20.5 1.0 CA A:CYS94 4.2 16.8 1.0 CD2 A:HIS62 4.2 16.7 1.0 OE1 A:GLU64 4.2 15.8 1.0 CD A:GLU64 4.3 15.0 1.0 N1 A:DUC300 4.3 16.6 1.0 C A:CYS91 4.7 18.5 1.0 O2 A:DUC300 4.7 17.2 1.0 CB A:MET93 4.8 18.3 1.0 O A:CYS91 4.8 16.0 1.0 C A:PRO90 4.9 18.2 1.0 C A:MET93 4.9 16.6 1.0 CA A:HIS62 5.0 17.8 1.0

Zinc binding site 2 out of 2 in the The Crystal Structure of Yeast Cytosine Deaminase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Crystal Structure of Yeast Cytosine Deaminase within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn200 b:18.9 occ:1.00 ND1 B:HIS62 2.1 18.9 1.0 O4 B:DUC300 2.1 19.2 1.0 SG B:CYS94 2.3 17.8 1.0 SG B:CYS91 2.3 19.3 1.0 CE1 B:HIS62 3.0 17.5 1.0 CG B:HIS62 3.1 19.4 1.0 C4 B:DUC300 3.1 20.6 1.0 CB B:CYS94 3.2 17.8 1.0 CB B:CYS91 3.4 21.9 1.0 CB B:HIS62 3.5 17.2 1.0 C5 B:DUC300 3.5 20.7 1.0 N3 B:DUC300 3.6 19.4 1.0 OE2 B:GLU64 3.8 21.5 1.0 N B:CYS91 3.8 19.0 1.0 N B:CYS94 3.8 16.3 1.0 C2 B:DUC300 4.1 17.6 1.0 NE2 B:HIS62 4.1 17.9 1.0 C6 B:DUC300 4.1 20.6 1.0 CA B:CYS94 4.1 17.6 1.0 CA B:CYS91 4.2 19.5 1.0 CD2 B:HIS62 4.2 18.3 1.0 CD B:GLU64 4.3 20.7 1.0 OE1 B:GLU64 4.3 19.2 1.0 N1 B:DUC300 4.3 19.7 1.0 CE B:MET93 4.3 18.1 1.0 C B:CYS91 4.7 18.8 1.0 O2 B:DUC300 4.7 19.4 1.0 CB B:MET93 4.8 19.0 1.0 O B:CYS91 4.8 18.7 1.0 C B:MET93 4.9 16.4 1.0 C B:PRO90 4.9 20.6 1.0 CA B:HIS62 5.0 17.8 1.0

T.-P.Ko, J.-J.Lin, C.-Y.Hu, Y.-H.Hsu, A.H.-J.Wang, S.-H.Liaw. Crystal Structure of Yeast Cytosine Deaminase. Insights Into Enzyme Mechanism and Evolution J.Biol.Chem. V. 278 19111 2003.
ISSN: ISSN 0021-9258
PubMed: 12637534
DOI: 10.1074/JBC.M300874200