Atomistry » Zinc » PDB 1tnz-1u1j » 1u0a
Atomistry »
  Zinc »
    PDB 1tnz-1u1j »
      1u0a »

Zinc in PDB 1u0a: Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide

Enzymatic activity of Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide

All present enzymatic activity of Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide:
3.2.1.73;

Protein crystallography data

The structure of Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide, PDB code: 1u0a was solved by O.J.Gaiser, K.Piotukh, M.N.Ponnuswamy, A.Planas, R.Borriss, U.Heinemann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 34.46 / 1.64
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 75.769, 88.758, 154.795, 90.00, 90.00, 90.00
R / Rfree (%) 16.1 / 19.7

Other elements in 1u0a:

The structure of Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide also contains other interesting chemical elements:

Calcium (Ca) 4 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide (pdb code 1u0a). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide, PDB code: 1u0a:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in 1u0a

Go back to Zinc Binding Sites List in 1u0a
Zinc binding site 1 out of 3 in the Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn5005

b:16.5
occ:1.00
 OD2 A:ASP161 1.9 16.2 1.0
OD2 C:ASP761 1.9 16.1 1.0
ND1 C:HIS745 2.0 15.5 1.0
ND1 A:HIS145 2.0 16.1 1.0
CG A:ASP161 2.9 16.5 1.0
CE1 C:HIS745 2.9 15.1 1.0
CE1 A:HIS145 2.9 16.0 1.0
CG C:ASP761 2.9 16.4 1.0
CG C:HIS745 3.1 15.4 1.0
CG A:HIS145 3.2 16.1 1.0
OD1 A:ASP161 3.2 18.3 1.0
OD1 C:ASP761 3.3 16.8 1.0
CB C:HIS745 3.6 15.4 1.0
CB A:HIS145 3.6 17.1 1.0
O A:HOH2172 3.9 16.8 1.0
O C:HOH2148 3.9 17.5 1.0
NE2 C:HIS745 4.0 16.3 1.0
NE2 A:HIS145 4.1 16.9 1.0
CD2 C:HIS745 4.2 16.1 1.0
CB A:ASP161 4.2 17.6 1.0
CB C:ASP761 4.2 17.9 1.0
CD2 A:HIS145 4.2 16.0 1.0
CA C:HIS745 4.3 15.7 1.0
CA A:HIS145 4.3 16.0 1.0
CE1 A:PHE138 4.7 16.1 1.0
CE1 C:PHE738 4.7 16.6 1.0
CZ A:PHE138 4.8 16.1 1.0
CZ C:PHE738 4.8 16.2 1.0
O A:PHE144 4.9 15.2 1.0
O C:PHE744 4.9 17.7 1.0

Zinc binding site 2 out of 3 in 1u0a

Go back to Zinc Binding Sites List in 1u0a
Zinc binding site 2 out of 3 in the Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn5011

b:23.2
occ:1.00
 O A:HOH3595 2.0 23.4 1.0
ND1 A:HIS166 2.0 16.3 1.0
O A:HOH6004 2.3 15.6 0.5
O A:HOH3596 2.3 17.7 0.5
CE1 A:HIS166 2.7 17.6 1.0
CG A:HIS166 3.3 15.9 1.0
CB A:HIS166 3.9 16.7 1.0
NE2 A:HIS166 3.9 16.6 1.0
CA A:HIS166 4.0 16.2 1.0
CE A:LYS132 4.0 20.3 1.0
O A:HOH2771 4.2 32.2 1.0
CD2 A:HIS166 4.2 17.5 1.0
CD A:LYS132 4.2 18.8 1.0
N A:THR167 4.3 17.7 1.0
NZ A:LYS132 4.4 21.8 1.0
CG A:LYS132 4.4 17.4 1.0
O A:LYS165 4.5 16.6 1.0
O A:HOH3522 4.5 40.3 0.5
O A:THR167 4.6 20.3 1.0
C A:HIS166 4.7 16.6 1.0
O A:HOH3467 4.8 30.7 0.5

Zinc binding site 3 out of 3 in 1u0a

Go back to Zinc Binding Sites List in 1u0a
Zinc binding site 3 out of 3 in the Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the Engineered Beta-1,3-1,4-Endoglucanase H(A16- M) in Complex with Beta-Glucan Tetrasaccharide within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn5006

b:13.8
occ:1.00
 OD2 D:ASP1061 1.8 14.3 1.0
OD2 B:ASP461 1.8 14.6 1.0
ND1 D:HIS1045 2.0 12.4 1.0
ND1 B:HIS445 2.1 14.9 1.0
CE1 D:HIS1045 2.8 13.3 1.0
CG D:ASP1061 2.8 14.5 1.0
CG B:ASP461 2.9 15.0 1.0
CE1 B:HIS445 2.9 14.5 1.0
CG D:HIS1045 3.1 12.7 1.0
CG B:HIS445 3.2 14.9 1.0
OD1 D:ASP1061 3.2 13.5 1.0
OD1 B:ASP461 3.2 15.5 1.0
CB B:HIS445 3.6 15.6 1.0
CB D:HIS1045 3.6 12.3 1.0
O B:HOH2157 4.0 17.9 1.0
NE2 D:HIS1045 4.0 11.8 1.0
O D:HOH2059 4.0 13.6 1.0
NE2 B:HIS445 4.1 15.2 1.0
CD2 D:HIS1045 4.2 13.6 1.0
CB D:ASP1061 4.2 14.2 1.0
CB B:ASP461 4.2 14.4 1.0
CD2 B:HIS445 4.2 15.1 1.0
CA B:HIS445 4.3 14.9 1.0
CA D:HIS1045 4.3 12.6 1.0
CE1 B:PHE438 4.7 15.1 1.0
CE1 D:PHE1038 4.7 13.9 1.0
O B:PHE444 4.8 16.0 1.0
CZ D:PHE1038 4.8 13.3 1.0
O D:PHE1044 4.8 14.9 1.0
CZ B:PHE438 4.9 13.9 1.0

Reference:

O.J.Gaiser, K.Piotukh, M.N.Ponnuswamy, A.Planas, R.Borriss, U.Heinemann. Structural Basis For the Substrate Specificity of A Bacillus 1,3-1,4-Beta-Glucanase J.Mol.Biol. V. 357 1211 2006.
ISSN: ISSN 0022-2836
PubMed: 16483609
DOI: 10.1016/J.JMB.2006.01.014
Page generated: Wed Oct 16 19:22:29 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy