Atomistry » Zinc » PDB 1tnz-1u1j » 1twg
Atomistry »
  Zinc »
    PDB 1tnz-1u1j »
      1twg »

Zinc in PDB 1twg: Rna Polymerase II Complexed with Ctp

Enzymatic activity of Rna Polymerase II Complexed with Ctp

All present enzymatic activity of Rna Polymerase II Complexed with Ctp:
2.7.7.6;

Protein crystallography data

The structure of Rna Polymerase II Complexed with Ctp, PDB code: 1twg was solved by K.D.Westover, D.A.Bushnell, R.D.Kornberg, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.00 / 3.30
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 123.000, 223.000, 374.000, 90.00, 90.00, 90.00
R / Rfree (%) 26.3 / 28.1

Other elements in 1twg:

The structure of Rna Polymerase II Complexed with Ctp also contains other interesting chemical elements:

Manganese (Mn) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Rna Polymerase II Complexed with Ctp (pdb code 1twg). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Rna Polymerase II Complexed with Ctp, PDB code: 1twg:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in 1twg

Go back to Zinc Binding Sites List in 1twg
Zinc binding site 1 out of 8 in the Rna Polymerase II Complexed with Ctp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Rna Polymerase II Complexed with Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn3006

b:48.2
occ:1.00
 SG A:CYS110 1.2 0.2 1.0
SG A:CYS107 1.5 0.7 1.0
SG A:CYS148 1.6 47.6 1.0
SG A:CYS167 2.2 0.5 1.0
CB A:CYS148 2.6 55.2 1.0
CB A:CYS107 2.7 0.6 1.0
CB A:CYS167 3.1 0.2 1.0
CB A:CYS110 3.2 0.1 1.0
O A:GLY166 3.9 39.4 1.0
CA A:CYS148 4.1 31.0 1.0
N A:CYS110 4.2 46.5 1.0
CA A:CYS107 4.3 42.4 1.0
CA A:CYS110 4.4 45.6 1.0
CA A:CYS167 4.4 78.3 1.0
O A:CYS148 4.7 31.3 1.0
OE1 A:GLN171 4.7 41.5 1.0
N A:GLY168 4.7 54.6 1.0
C A:CYS107 4.8 40.8 1.0
C A:CYS148 4.9 31.4 1.0
C A:GLY166 4.9 40.8 1.0
C A:CYS167 5.0 74.3 1.0
N A:GLY111 5.0 51.5 1.0

Zinc binding site 2 out of 8 in 1twg

Go back to Zinc Binding Sites List in 1twg
Zinc binding site 2 out of 8 in the Rna Polymerase II Complexed with Ctp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Rna Polymerase II Complexed with Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn3008

b:87.6
occ:1.00
 SG A:CYS70 1.2 0.8 1.0
NE2 A:HIS80 1.7 0.9 1.0
SG A:CYS77 2.1 52.0 1.0
CD2 A:HIS80 2.5 0.7 1.0
CE1 A:HIS80 3.0 0.3 1.0
CB A:CYS70 3.0 0.4 1.0
SG A:CYS67 3.1 0.7 1.0
CB A:CYS67 3.5 0.1 1.0
CB A:CYS77 3.6 69.0 1.0
CG A:HIS80 3.8 0.7 1.0
ND1 A:HIS80 4.0 0.4 1.0
CA A:CYS77 4.3 77.6 1.0
CA A:CYS70 4.4 0.2 1.0
C A:CYS70 4.5 0.3 1.0
O A:CYS70 4.8 0.6 1.0
CD A:PRO78 4.9 68.7 1.0
N A:GLN71 5.0 85.4 1.0

Zinc binding site 3 out of 8 in 1twg

Go back to Zinc Binding Sites List in 1twg
Zinc binding site 3 out of 8 in the Rna Polymerase II Complexed with Ctp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Rna Polymerase II Complexed with Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn3007

b:37.9
occ:1.00
 SG B:CYS1185 0.9 0.0 1.0
CB B:CYS1185 1.9 0.0 1.0
SG B:CYS1182 2.1 51.0 1.0
SG B:CYS1163 2.2 50.7 1.0
SG B:CYS1166 2.2 33.3 1.0
CB B:CYS1163 2.8 44.0 1.0
CB B:CYS1166 2.9 37.2 1.0
CB B:CYS1182 3.2 73.4 1.0
CA B:CYS1185 3.6 47.7 1.0
N B:CYS1166 4.3 29.9 1.0
N B:CYS1185 4.3 49.5 1.0
CA B:CYS1166 4.4 31.1 1.0
OG1 B:THR1170 4.5 19.8 1.0
CG1 B:ILE1165 4.6 73.6 1.0
CA B:CYS1163 4.6 36.0 1.0
CB B:ILE1165 4.7 70.8 1.0
CA B:CYS1182 4.8 94.8 1.0
C B:CYS1185 4.9 49.1 1.0

Zinc binding site 4 out of 8 in 1twg

Go back to Zinc Binding Sites List in 1twg
Zinc binding site 4 out of 8 in the Rna Polymerase II Complexed with Ctp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Rna Polymerase II Complexed with Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn3002

b:35.2
occ:1.00
 SG C:CYS86 1.3 9.5 1.0
SG C:CYS95 1.9 28.4 1.0
SG C:CYS92 2.2 16.4 1.0
SG C:CYS88 2.2 23.4 1.0
CB C:CYS88 2.7 26.1 1.0
CB C:CYS95 2.9 26.2 1.0
CB C:CYS86 3.3 20.9 1.0
CB C:CYS92 3.4 25.8 1.0
N C:CYS88 3.8 6.6 1.0
N C:CYS92 3.8 46.4 1.0
CA C:CYS88 3.9 11.4 1.0
N C:CYS95 4.0 36.4 1.0
CA C:CYS95 4.2 35.8 1.0
C C:HIS91 4.2 64.7 1.0
CA C:CYS92 4.2 47.2 1.0
O C:CYS92 4.5 47.0 1.0
CA C:HIS91 4.5 63.0 1.0
CA C:CYS86 4.6 29.4 1.0
N C:LYS94 4.6 28.3 1.0
C C:CYS92 4.6 48.2 1.0
C C:LYS94 4.7 29.7 1.0
C C:CYS86 4.8 28.3 1.0
O C:HIS91 4.9 68.7 1.0
CB C:LYS94 5.0 79.3 1.0
C C:CYS88 5.0 9.5 1.0
CA C:LYS94 5.0 32.2 1.0

Zinc binding site 5 out of 8 in 1twg

Go back to Zinc Binding Sites List in 1twg
Zinc binding site 5 out of 8 in the Rna Polymerase II Complexed with Ctp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Rna Polymerase II Complexed with Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn3003

b:46.0
occ:1.00
 SG I:CYS32 1.1 0.3 1.0
SG I:CYS7 1.5 9.6 1.0
SG I:CYS10 1.7 50.4 1.0
SG I:CYS29 2.1 42.3 1.0
CB I:CYS32 2.5 0.5 1.0
CB I:CYS7 2.8 24.0 1.0
CB I:CYS29 3.3 41.1 1.0
N I:CYS32 3.5 58.9 1.0
CA I:CYS32 3.6 56.6 1.0
CB I:CYS10 3.6 52.6 1.0
CG2 I:THR31 4.0 35.1 1.0
N I:CYS10 4.1 56.8 1.0
CB I:TYR34 4.3 2.0 1.0
CB I:THR31 4.4 28.6 1.0
CA I:CYS7 4.4 18.9 1.0
CA I:CYS10 4.5 53.7 1.0
C I:THR31 4.5 34.5 1.0
C I:CYS32 4.5 59.1 1.0
CA I:CYS29 4.7 21.8 1.0
N I:TYR34 4.9 1.9 1.0
CA I:THR31 4.9 32.6 1.0
N I:THR31 5.0 31.3 1.0

Zinc binding site 6 out of 8 in 1twg

Go back to Zinc Binding Sites List in 1twg
Zinc binding site 6 out of 8 in the Rna Polymerase II Complexed with Ctp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Rna Polymerase II Complexed with Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn3004

b:49.1
occ:1.00
 SG I:CYS106 1.3 48.5 1.0
SG I:CYS78 1.6 50.6 1.0
SG I:CYS75 1.8 42.2 1.0
SG I:CYS103 2.6 58.1 1.0
CB I:CYS78 3.1 71.8 1.0
CB I:CYS106 3.1 65.5 1.0
CB I:CYS103 3.2 48.2 1.0
CB I:CYS75 3.2 59.5 1.0
N I:CYS78 3.2 91.1 1.0
CB I:LYS77 3.7 62.4 1.0
CA I:CYS78 3.8 86.3 1.0
CB I:HIS108 3.8 55.0 1.0
N I:LYS77 4.2 41.7 1.0
CA I:LYS77 4.2 42.4 1.0
C I:LYS77 4.2 41.6 1.0
CA I:CYS106 4.5 56.3 1.0
CA I:CYS75 4.6 39.9 1.0
CG I:HIS108 4.6 56.8 1.0
N I:HIS108 4.7 38.6 1.0
CA I:CYS103 4.7 17.8 1.0
N I:CYS106 4.8 61.3 1.0
C I:CYS106 4.8 55.9 1.0
CD2 I:HIS108 4.8 61.6 1.0
CA I:HIS108 4.9 37.1 1.0
C I:CYS78 4.9 87.0 1.0
O I:CYS106 5.0 56.6 1.0
N I:SER107 5.0 22.7 1.0

Zinc binding site 7 out of 8 in 1twg

Go back to Zinc Binding Sites List in 1twg
Zinc binding site 7 out of 8 in the Rna Polymerase II Complexed with Ctp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Rna Polymerase II Complexed with Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn3001

b:31.7
occ:1.00
 SG J:CYS45 1.5 61.2 1.0
SG J:CYS7 1.6 31.8 1.0
SG J:CYS10 1.9 24.3 1.0
SG J:CYS46 2.3 1.0 1.0
N J:CYS46 2.8 14.8 1.0
CB J:CYS7 2.9 28.2 1.0
CB J:CYS45 3.0 62.3 1.0
CB J:CYS10 3.0 26.5 1.0
C J:CYS45 3.6 8.6 1.0
CA J:CYS46 3.7 13.8 1.0
CB J:CYS46 3.8 1.0 1.0
N J:CYS10 3.8 35.3 1.0
CA J:CYS45 3.9 6.5 1.0
CA J:CYS10 4.0 33.8 1.0
NE J:ARG43 4.2 43.5 1.0
O J:CYS45 4.3 3.7 1.0
CA J:CYS7 4.6 14.7 1.0
N J:CYS45 4.6 6.9 1.0
CG J:ARG43 4.6 43.0 1.0
CB J:SER9 4.8 30.5 1.0
C J:SER9 4.8 7.8 1.0
N J:SER9 5.0 1.4 1.0
CZ J:ARG43 5.0 40.2 1.0

Zinc binding site 8 out of 8 in 1twg

Go back to Zinc Binding Sites List in 1twg
Zinc binding site 8 out of 8 in the Rna Polymerase II Complexed with Ctp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Rna Polymerase II Complexed with Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Zn3005

b:84.7
occ:1.00
 SG L:CYS31 1.7 0.6 1.0
SG L:CYS34 1.8 25.0 1.0
SG L:CYS48 1.9 71.5 1.0
SG L:CYS51 2.1 89.4 1.0
CB L:CYS51 2.7 0.5 1.0
CB L:CYS34 2.9 52.0 1.0
CB L:CYS31 3.1 0.4 1.0
CB L:CYS48 3.5 90.9 1.0
OG L:SER36 3.6 51.5 1.0
O L:CYS51 3.6 1.0 1.0
CA L:CYS51 3.7 0.5 1.0
N L:CYS51 3.8 0.8 1.0
C L:CYS51 4.0 0.7 1.0
CA L:CYS34 4.0 67.0 1.0
N L:CYS34 4.1 74.1 1.0
C L:CYS34 4.4 66.4 1.0
O L:CYS34 4.6 66.7 1.0
CA L:CYS31 4.6 0.2 1.0
N L:HIS53 4.6 0.8 1.0
N L:LYS49 4.7 98.7 1.0
CA L:CYS48 4.8 0.2 1.0
CB L:HIS53 4.9 0.3 1.0
N L:SER35 4.9 47.5 1.0
N L:ASP50 5.0 0.8 1.0

Reference:

K.D.Westover, D.A.Bushnell, R.D.Kornberg. Structural Basis of Transcription: Nucleotide Selection By Rotation in the Rna Polymerase II Active Center. Cell(Cambridge,Mass.) V. 119 481 2004.
ISSN: ISSN 0092-8674
PubMed: 15537538
DOI: 10.1016/J.CELL.2004.10.016
Page generated: Wed Dec 16 03:05:51 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy