Zinc in PDB 1ro2: Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak

The structure of Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak, PDB code: 1ro2 was solved by G.Lipps, A.O.Weinzierl, G.Von Scheven, C.Buchen, P.Cramer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.60
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	45.811, 119.907, 41.830, 90.00, 90.00, 90.00
R / Rfree (%)	25.2 / 26.5

The structure of Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak also contains other interesting chemical elements:

Manganese

(Mn)

1 atom

The binding sites of Zinc atom in the Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak (pdb code 1ro2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak, PDB code: 1ro2:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:44.6 occ:1.00 ND1 A:HIS188 2.2 18.9 1.0 O A:HOH516 2.3 40.4 1.0 O A:HOH515 2.4 39.2 1.0 NE2 A:HIS141 2.4 28.4 1.0 CE1 A:HIS188 3.1 16.4 1.0 CD2 A:HIS141 3.2 27.7 1.0 CG A:HIS188 3.2 16.8 1.0 CE1 A:HIS141 3.6 29.4 1.0 CB A:HIS188 3.6 16.6 1.0 CB A:CYS191 3.8 30.4 1.0 SG A:CYS191 4.0 37.2 1.0 NE2 A:HIS188 4.2 16.4 1.0 CA A:HIS188 4.2 17.8 1.0 CD2 A:HIS188 4.3 16.6 1.0 CG A:HIS141 4.4 25.6 1.0 ND1 A:HIS141 4.6 30.6 1.0 CD1 A:TRP198 4.6 20.6 1.0 CG1 A:VAL186 4.8 24.4 1.0 CB A:TRP198 4.9 18.0 1.0 CA A:CYS196 5.0 36.9 1.0

Zinc binding site 2 out of 2 in the Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bifunctional Dna Primase/Polymerase Domain of ORF904 From the Archaeal Plasmid PRN1- Triple Mutant F50M/L107M/L110M Manganese Soak within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn302 b:35.0 occ:1.00 NE2 A:HIS53 2.2 24.5 1.0 OE2 A:GLU215 2.5 36.9 1.0 O A:HOH406 2.7 40.9 1.0 CD A:GLU215 2.8 32.1 1.0 O A:HOH332 2.9 29.4 1.0 OE1 A:GLU215 3.0 36.1 1.0 CD2 A:HIS53 3.1 22.9 1.0 CE1 A:HIS53 3.3 24.1 1.0 CG A:GLU215 3.7 30.4 1.0 CG A:HIS53 4.3 21.6 1.0 ND1 A:HIS53 4.3 22.4 1.0 OE2 A:GLU52 4.4 26.9 1.0 NE1 A:TRP49 4.8 18.0 1.0 CB A:GLU215 4.9 23.6 1.0

G.Lipps, A.O.Weinzierl, G.Von Scheven, C.Buchen, P.Cramer. Structure of A Bifunctional Dna Primase-Polymerase Nat.Struct.Mol.Biol. V. 11 157 2004.
ISSN: ISSN 1545-9993
PubMed: 14730355
DOI: 10.1038/NSMB723