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Zinc in PDB 1qv7: Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol

Enzymatic activity of Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol

All present enzymatic activity of Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol:
1.1.1.1;

Protein crystallography data

The structure of Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol, PDB code: 1qv7 was solved by L.A.Lebrun, D.-H.Park, S.Ramaswamy, B.V.Plapp, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.80
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 44.050, 50.962, 92.466, 91.61, 103.03, 109.86
R / Rfree (%) 17.7 / 22.2

Other elements in 1qv7:

The structure of Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol also contains other interesting chemical elements:

Fluorine (F) 4 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol (pdb code 1qv7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol, PDB code: 1qv7:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 1qv7

Go back to Zinc Binding Sites List in 1qv7
Zinc binding site 1 out of 4 in the Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn375

b:34.5
occ:1.00
 NE2 A:HIS67 2.1 20.9 1.0
SG A:CYS174 2.2 21.8 1.0
O1 A:DFB378 2.2 30.6 1.0
SG A:CYS46 2.4 29.8 1.0
CD2 A:HIS67 3.1 18.6 1.0
CE1 A:HIS67 3.1 21.5 1.0
C7 A:DFB378 3.1 34.1 1.0
CB A:CYS174 3.3 17.5 1.0
CB A:CYS46 3.3 24.7 1.0
C5N A:NAD377 3.4 19.0 1.0
C6N A:NAD377 4.0 18.2 1.0
C4N A:NAD377 4.2 16.3 1.0
ND1 A:HIS67 4.2 17.3 1.0
CG A:HIS67 4.2 17.4 1.0
OG A:SER48 4.2 24.1 1.0
CB A:SER48 4.3 22.5 1.0
C1 A:DFB378 4.5 34.4 1.0
NH2 A:ARG369 4.5 36.7 1.0
CA A:CYS174 4.6 16.7 1.0
CE2 A:PHE93 4.8 12.5 1.0
CA A:CYS46 4.8 23.6 1.0
N A:GLY175 4.8 15.2 1.0
OE2 A:GLU68 4.8 24.0 1.0
C A:CYS174 4.9 15.4 1.0

Zinc binding site 2 out of 4 in 1qv7

Go back to Zinc Binding Sites List in 1qv7
Zinc binding site 2 out of 4 in the Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn376

b:18.3
occ:1.00
 SG A:CYS103 2.3 16.9 1.0
SG A:CYS97 2.3 18.3 1.0
SG A:CYS111 2.3 15.8 1.0
SG A:CYS100 2.4 19.2 1.0
CB A:CYS111 3.3 17.1 1.0
CB A:CYS103 3.4 15.2 1.0
CB A:CYS97 3.4 19.4 1.0
CB A:CYS100 3.5 19.0 1.0
N A:CYS97 3.6 15.2 1.0
CA A:CYS111 3.7 16.5 1.0
N A:CYS100 3.9 20.4 1.0
N A:GLY98 3.9 18.1 1.0
CA A:CYS97 3.9 18.4 1.0
N A:LEU112 4.0 15.9 1.0
N A:CYS103 4.1 16.5 1.0
CA A:CYS100 4.2 19.9 1.0
C A:CYS97 4.3 19.4 1.0
C A:CYS111 4.3 17.4 1.0
CA A:CYS103 4.3 16.2 1.0
N A:LYS99 4.5 22.2 1.0
C A:GLN96 4.6 17.0 1.0
N A:LYS113 4.8 17.9 1.0
C A:CYS100 4.8 17.6 1.0
CA A:GLN96 4.9 16.0 1.0
CG A:LYS113 4.9 23.5 1.0
CA A:GLY98 4.9 20.5 1.0
O A:CYS100 4.9 16.9 1.0

Zinc binding site 3 out of 4 in 1qv7

Go back to Zinc Binding Sites List in 1qv7
Zinc binding site 3 out of 4 in the Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn375

b:37.3
occ:1.00
 NE2 B:HIS67 2.1 24.5 1.0
SG B:CYS174 2.2 26.3 1.0
O1 B:DFB378 2.3 33.3 1.0
SG B:CYS46 2.4 34.4 1.0
CD2 B:HIS67 3.0 25.1 1.0
CE1 B:HIS67 3.1 23.1 1.0
C7 B:DFB378 3.2 40.2 1.0
CB B:CYS46 3.3 28.6 1.0
CB B:CYS174 3.3 21.0 1.0
C5N B:NAD377 3.4 24.2 1.0
C6N B:NAD377 4.1 23.7 1.0
ND1 B:HIS67 4.2 22.4 1.0
CG B:HIS67 4.2 20.7 1.0
CB B:SER48 4.2 25.5 1.0
C4N B:NAD377 4.2 23.2 1.0
OG B:SER48 4.2 27.5 1.0
NH2 B:ARG369 4.5 35.5 1.0
C1 B:DFB378 4.6 42.0 1.0
CA B:CYS174 4.6 20.3 1.0
CA B:CYS46 4.8 28.4 1.0
N B:GLY175 4.8 19.5 1.0
OE2 B:GLU68 4.9 30.9 1.0
CE2 B:PHE93 4.9 19.7 1.0
C B:CYS174 5.0 20.4 1.0

Zinc binding site 4 out of 4 in 1qv7

Go back to Zinc Binding Sites List in 1qv7
Zinc binding site 4 out of 4 in the Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Horse Liver Alcohol Dehydrogenase HIS51GLN/LYS228ARG Mutant Complexed with Nad+ and 2,3-Difluorobenzyl Alcohol within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn376

b:22.1
occ:1.00
 SG B:CYS103 2.3 20.6 1.0
SG B:CYS97 2.3 23.7 1.0
SG B:CYS100 2.4 20.5 1.0
SG B:CYS111 2.4 20.2 1.0
CB B:CYS111 3.2 18.5 1.0
CB B:CYS103 3.3 19.1 1.0
CB B:CYS97 3.4 25.2 1.0
CB B:CYS100 3.4 23.2 1.0
N B:CYS97 3.5 23.3 1.0
CA B:CYS111 3.7 18.6 1.0
N B:CYS100 3.9 24.5 1.0
CA B:CYS97 3.9 24.3 1.0
N B:GLY98 3.9 25.7 1.0
N B:LEU112 3.9 19.4 1.0
CA B:CYS100 4.2 23.1 1.0
C B:CYS111 4.2 18.7 1.0
N B:CYS103 4.2 19.6 1.0
C B:CYS97 4.3 26.0 1.0
CA B:CYS103 4.3 19.4 1.0
N B:LYS99 4.4 25.9 1.0
C B:GLN96 4.6 21.9 1.0
C B:CYS100 4.8 22.6 1.0
N B:LYS113 4.9 20.9 1.0
CA B:GLY98 4.9 25.7 1.0
CA B:GLN96 4.9 21.1 1.0
CG B:LYS113 4.9 25.7 1.0
O B:CYS100 4.9 19.9 1.0

Reference:

L.A.Lebrun, D.-H.Park, S.Ramaswamy, B.V.Plapp. Participation of Histidine-51 in Catalysis By Horse Liver Alcohol Dehydrogenase. Biochemistry V. 43 3014 2004.
ISSN: ISSN 0006-2960
PubMed: 15023053
DOI: 10.1021/BI036103M
Page generated: Wed Oct 16 18:16:13 2024

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