Zinc in PDB 1qiz: Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol

The structure of Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol, PDB code: 1qiz was solved by L.Tang, J.L.Whittingham, C.S.Verma, L.S.D.Caves, G.G.Dodson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.20 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	60.810, 62.050, 47.650, 90.00, 110.40, 90.00
R / Rfree (%)	19.1 / n/a

The structure of Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol (pdb code 1qiz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol, PDB code: 1qiz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1001 b:24.0 occ:1.00 NE2 F:HIS10 2.0 22.3 1.0 NE2 J:HIS10 2.1 19.4 1.0 NE2 B:HIS10 2.1 17.1 1.0 CL J:CL1002 2.2 26.0 1.0 CE1 F:HIS10 3.0 25.0 1.0 CE1 J:HIS10 3.0 27.8 1.0 CE1 B:HIS10 3.0 24.5 1.0 CD2 F:HIS10 3.1 18.5 1.0 CD2 B:HIS10 3.1 15.6 1.0 CD2 J:HIS10 3.1 20.4 1.0 ND1 F:HIS10 4.1 25.8 1.0 ND1 J:HIS10 4.1 24.4 1.0 ND1 B:HIS10 4.1 22.9 1.0 CG F:HIS10 4.2 24.7 1.0 CG B:HIS10 4.2 21.8 1.0 CG J:HIS10 4.2 26.9 1.0 O F:LEU6 4.8 25.2 1.0 O B:LEU6 4.9 21.9 1.0 O J:LEU6 4.9 20.1 1.0 O F:HOH2006 4.9 47.4 1.0

Zinc binding site 2 out of 2 in the Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Human Insulin Hexamers with Chain B His Mutated to Tyr Complexed with Resorcinol within 5.0Å range: probe atom residue distance (Å) B Occ L:Zn1001 b:24.4 occ:1.00 NE2 D:HIS10 2.0 21.0 1.0 NE2 L:HIS10 2.0 22.0 1.0 NE2 H:HIS10 2.1 30.2 1.0 CL L:CL1002 2.2 26.4 1.0 CE1 L:HIS10 2.9 20.6 1.0 CE1 D:HIS10 3.0 20.1 1.0 CE1 H:HIS10 3.0 30.1 1.0 CD2 D:HIS10 3.1 21.6 1.0 CD2 H:HIS10 3.1 22.8 1.0 CD2 L:HIS10 3.1 20.0 1.0 ND1 D:HIS10 4.1 23.9 1.0 ND1 L:HIS10 4.1 23.7 1.0 CG D:HIS10 4.2 22.8 1.0 ND1 H:HIS10 4.2 26.5 1.0 CG L:HIS10 4.2 21.4 1.0 CG H:HIS10 4.2 26.9 1.0 O D:LEU6 4.8 32.1 1.0 O H:LEU6 4.9 25.2 1.0 O L:LEU6 5.0 25.4 1.0

L.Tang, J.L.Whittingham, C.S.Verma, L.S.D.Caves, G.G.Dodson. Structural Consequences of the B5 Histidine --> Tyrosine Mutation in Human Insulin Characterized By X-Ray Crystallography and Conformational Analysis. Biochemistry V. 38 12041 1999.
ISSN: ISSN 0006-2960
PubMed: 10508408
DOI: 10.1021/BI990700K