Zinc in PDB 1os3: Dehydrated T6 Human Insulin at 100 K

The structure of Dehydrated T6 Human Insulin at 100 K, PDB code: 1os3 was solved by G.D.Smith, R.H.Blessing, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	16.42 / 1.95
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	81.510, 81.510, 33.760, 90.00, 90.00, 120.00
R / Rfree (%)	21.6 / 25.3

The structure of Dehydrated T6 Human Insulin at 100 K also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the Dehydrated T6 Human Insulin at 100 K (pdb code 1os3). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Dehydrated T6 Human Insulin at 100 K, PDB code: 1os3:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Dehydrated T6 Human Insulin at 100 K


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Dehydrated T6 Human Insulin at 100 K within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn501 b:22.7 occ:0.33 NE2 B:HIS10 2.0 19.4 1.0 O B:HOH505 2.2 18.2 0.5 CL B:CL503 2.4 44.7 0.2 CE1 B:HIS10 3.0 24.0 1.0 CD2 B:HIS10 3.1 22.8 1.0 ND1 B:HIS10 4.1 24.8 1.0 CG B:HIS10 4.2 21.9 1.0 O B:HOH616 4.5 42.8 1.0

Zinc binding site 2 out of 2 in the Dehydrated T6 Human Insulin at 100 K


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Dehydrated T6 Human Insulin at 100 K within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn502 b:23.0 occ:0.33 NE2 D:HIS10 2.0 17.5 1.0 CL D:CL504 2.4 35.2 0.2 O D:HOH506 2.5 20.2 0.5 CE1 D:HIS10 2.9 20.1 1.0 CD2 D:HIS10 3.1 18.3 1.0 ND1 D:HIS10 4.1 21.7 1.0 CG D:HIS10 4.2 19.9 1.0 O D:HOH635 4.9 57.5 1.0 O D:HOH627 4.9 49.6 0.3

G.D.Smith, R.H.Blessing. Lessons From An Aged, Dried Crystal of T(6) Human Insulin. Acta Crystallogr.,Sect.D V. 59 1384 2003.
ISSN: ISSN 0907-4449
PubMed: 12876340
DOI: 10.1107/S090744490301165X