Zinc in PDB 1ocz: Bovine Heart Cytochrome C Oxidase in Azide-Bound State

All present enzymatic activity of Bovine Heart Cytochrome C Oxidase in Azide-Bound State:
1.9.3.1;

The structure of Bovine Heart Cytochrome C Oxidase in Azide-Bound State, PDB code: 1ocz was solved by T.Tsukihara, M.Yao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	7.00 / 2.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	189.200, 210.600, 178.500, 90.00, 90.00, 90.00
R / Rfree (%)	19.5 / 25.5

The structure of Bovine Heart Cytochrome C Oxidase in Azide-Bound State also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Iron	(Fe)	4 atoms
Copper	(Cu)	6 atoms
Sodium	(Na)	2 atoms

The binding sites of Zinc atom in the Bovine Heart Cytochrome C Oxidase in Azide-Bound State (pdb code 1ocz). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Bovine Heart Cytochrome C Oxidase in Azide-Bound State, PDB code: 1ocz:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Bovine Heart Cytochrome C Oxidase in Azide-Bound State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Bovine Heart Cytochrome C Oxidase in Azide-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn99 b:24.0 occ:1.00 SG F:CYS82 2.1 27.4 1.0 SG F:CYS85 2.2 29.0 1.0 SG F:CYS60 2.2 26.5 1.0 SG F:CYS62 2.2 45.8 1.0 CB F:CYS82 3.0 30.4 1.0 CB F:CYS60 3.2 9.6 1.0 CB F:CYS62 3.5 23.4 1.0 OG F:SER84 3.5 57.4 1.0 CA F:CYS62 3.7 33.0 1.0 CB F:CYS85 3.8 23.3 1.0 N F:CYS62 4.0 26.4 1.0 N F:CYS85 4.0 34.3 1.0 OG1 F:THR87 4.1 38.2 1.0 O F:CYS60 4.3 34.6 1.0 C F:CYS60 4.4 26.2 1.0 CA F:CYS60 4.4 13.3 1.0 CA F:CYS82 4.5 28.9 1.0 CA F:CYS85 4.5 32.4 1.0 C F:SER84 4.7 34.5 1.0 CB F:THR87 4.8 41.5 1.0 CB F:SER84 4.8 39.0 1.0 C F:CYS82 4.9 27.2 1.0 N F:SER84 4.9 29.3 1.0 C F:ILE61 4.9 30.7 1.0 CB F:ILE70 4.9 13.9 1.0 O F:CYS82 5.0 28.6 1.0

Zinc binding site 2 out of 2 in the Bovine Heart Cytochrome C Oxidase in Azide-Bound State


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Bovine Heart Cytochrome C Oxidase in Azide-Bound State within 5.0Å range: probe atom residue distance (Å) B Occ S:Zn99 b:34.8 occ:1.00 SG S:CYS85 2.1 46.4 1.0 SG S:CYS60 2.2 34.0 1.0 SG S:CYS82 2.2 41.1 1.0 SG S:CYS62 2.3 41.9 1.0 CB S:CYS82 3.1 37.4 1.0 CB S:CYS60 3.2 25.9 1.0 CB S:CYS62 3.4 37.5 1.0 CA S:CYS62 3.6 38.0 1.0 CB S:CYS85 3.7 38.9 1.0 OG S:SER84 3.7 50.1 1.0 OG1 S:THR87 3.9 48.1 1.0 N S:CYS62 3.9 34.0 1.0 N S:CYS85 4.1 32.4 1.0 CA S:CYS85 4.4 38.0 1.0 CA S:CYS60 4.5 26.0 1.0 C S:CYS60 4.5 29.2 1.0 O S:CYS60 4.5 33.7 1.0 CA S:CYS82 4.6 33.9 1.0 CB S:THR87 4.6 43.2 1.0 C S:SER84 4.8 35.9 1.0 C S:ILE61 4.9 35.2 1.0 C S:CYS85 5.0 39.0 1.0 C S:CYS62 5.0 44.6 1.0 CB S:SER84 5.0 35.4 1.0

S.Yoshikawa, K.Shinzawa-Itoh, R.Nakashima, R.Yaono, E.Yamashita, N.Inoue, M.Yao, M.J.Fei, C.P.Libeu, T.Mizushima, H.Yamaguchi, T.Tomizaki, T.Tsukihara. Redox-Coupled Crystal Structural Changes in Bovine Heart Cytochrome C Oxidase. Science V. 280 1723 1998.
ISSN: ISSN 0036-8075
PubMed: 9624044
DOI: 10.1126/SCIENCE.280.5370.1723