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Zinc in PDB 1i97: Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline

Protein crystallography data

The structure of Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline, PDB code: 1i97 was solved by M.Pioletti, F.Schluenzen, J.Harms, R.Zarivach, M.Gluehmann, H.Avila, H.Bartels, C.Jacobi, T.Hartsch, A.Yonath, F.Franceschi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 35.00 / 4.50
Space group P 41 21 2
Cell size a, b, c (Å), α, β, γ (°) 406.900, 406.900, 175.200, 90.00, 90.00, 90.00
R / Rfree (%) 22.3 / 25.4

Other elements in 1i97:

The structure of Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline also contains other interesting chemical elements:

Tungsten (W) 252 atoms
Magnesium (Mg) 75 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline (pdb code 1i97). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline, PDB code: 1i97:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 1i97

Go back to Zinc Binding Sites List in 1i97
Zinc binding site 1 out of 2 in the Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline within 5.0Å range:
probe atom residue distance (Å) B Occ
N:Zn76

b:71.2
occ:1.00
CA N:CYS40 3.3 83.6 1.0
CA N:LEU39 3.6 92.3 1.0
CA N:CYS43 3.8 74.3 1.0
CA N:ARG26 3.9 96.1 1.0
CA N:CYS27 4.3 90.0 1.0
CA N:CYS24 4.3 0.7 1.0

Zinc binding site 2 out of 2 in 1i97

Go back to Zinc Binding Sites List in 1i97
Zinc binding site 2 out of 2 in the Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the 30S Ribosomal Subunit From Thermus Thermophilus in Complex with Tetracycline within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn212

b:71.2
occ:1.00
CA D:CYS9 4.0 0.7 1.0
CA D:CYS31 4.2 0.7 1.0
CA D:CYS26 4.6 0.6 1.0
CA D:CYS12 4.8 85.9 1.0

Reference:

M.Pioletti, F.Schlunzen, J.Harms, R.Zarivach, M.Gluhmann, H.Avila, A.Bashan, H.Bartels, T.Auerbach, C.Jacobi, T.Hartsch, A.Yonath, F.Franceschi. Crystal Structures of Complexes of the Small Ribosomal Subunit with Tetracycline, Edeine and IF3. Embo J. V. 20 1829 2001.
ISSN: ISSN 0261-4189
PubMed: 11296217
DOI: 10.1093/EMBOJ/20.8.1829
Page generated: Wed Dec 16 02:52:35 2020

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