Zinc in PDB 1hu8: Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution

The structure of Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution, PDB code: 1hu8 was solved by K.Zhao, X.Chai, K.Johnston, A.Clements, R.Marmorstein, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.70
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	73.676, 119.992, 184.238, 90.00, 90.00, 90.00
R / Rfree (%)	23.9 / 29.9

The binding sites of Zinc atom in the Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution (pdb code 1hu8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution, PDB code: 1hu8:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:60.9 occ:1.00 ND1 A:HIS176 1.9 48.9 1.0 SG A:CYS235 2.1 48.9 1.0 SG A:CYS173 2.3 45.5 1.0 SG A:CYS239 2.5 67.6 1.0 CE1 A:HIS176 2.5 50.3 1.0 CG A:HIS176 2.7 53.1 1.0 CB A:CYS239 3.3 66.6 1.0 CB A:HIS176 3.4 49.5 1.0 NE2 A:HIS176 3.4 50.1 1.0 CB A:CYS235 3.5 54.1 1.0 CD2 A:HIS176 3.6 48.3 1.0 CB A:CYS173 3.6 53.4 1.0 CA A:CYS235 3.7 55.8 1.0 N A:CYS173 4.0 51.2 1.0 N A:ASN236 4.1 55.2 1.0 N A:HIS176 4.2 55.5 1.0 C A:CYS235 4.4 55.0 1.0 CA A:CYS173 4.4 55.4 1.0 CA A:HIS176 4.5 51.2 1.0 O A:MET234 4.7 46.1 1.0 CA A:CYS239 4.8 70.9 1.0 O A:CYS173 4.9 65.5 1.0 O A:ASN236 4.9 53.5 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn502 b:53.7 occ:1.00 ND1 B:HIS176 2.1 55.6 1.0 SG B:CYS173 2.1 41.6 1.0 SG B:CYS235 2.2 50.1 1.0 SG B:CYS239 2.4 56.7 1.0 CE1 B:HIS176 2.9 63.9 1.0 CB B:CYS239 3.1 55.3 1.0 CG B:HIS176 3.1 54.4 1.0 CB B:CYS173 3.3 45.3 1.0 CB B:HIS176 3.6 45.5 1.0 CB B:CYS235 3.6 40.1 1.0 CA B:CYS235 3.9 46.2 1.0 N B:CYS173 3.9 51.4 1.0 N B:ASN236 4.0 57.0 1.0 NE2 B:HIS176 4.0 57.0 1.0 CD2 B:HIS176 4.2 49.4 1.0 CA B:CYS173 4.2 50.7 1.0 N B:HIS176 4.3 44.0 1.0 C B:CYS235 4.5 52.0 1.0 CA B:HIS176 4.5 42.2 1.0 CA B:CYS239 4.6 59.4 1.0 O B:ASN236 4.8 61.4 1.0 C B:CYS173 4.9 49.9 1.0 O B:CYS173 4.9 49.9 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn503 b:66.5 occ:1.00 SG C:CYS235 2.0 57.7 1.0 ND1 C:HIS176 2.3 81.8 1.0 SG C:CYS173 2.4 65.8 1.0 SG C:CYS239 2.8 88.3 1.0 CE1 C:HIS176 2.9 85.9 1.0 CG C:HIS176 2.9 80.2 1.0 CB C:CYS235 3.3 63.1 1.0 CB C:CYS239 3.3 93.3 1.0 CB C:CYS173 3.3 55.7 1.0 CB C:HIS176 3.5 75.5 1.0 CA C:CYS235 3.6 65.0 1.0 NE2 C:HIS176 3.6 85.2 1.0 CD2 C:HIS176 3.7 83.1 1.0 N C:CYS173 3.8 58.0 1.0 N C:ASN236 3.8 75.1 1.0 CA C:CYS173 4.1 62.6 1.0 C C:CYS235 4.2 68.9 1.0 N C:HIS176 4.3 59.9 1.0 O C:ASN236 4.4 81.5 1.0 CA C:HIS176 4.6 66.5 1.0 O C:CYS173 4.8 66.3 1.0 CA C:CYS239 4.9 94.1 1.0 O C:MET234 4.9 54.7 1.0 C C:CYS173 4.9 62.8 1.0 C C:ARG172 4.9 50.4 1.0 CA C:ASN236 4.9 76.8 1.0 N C:CYS235 4.9 59.9 1.0

K.Zhao, X.Chai, K.Johnston, A.Clements, R.Marmorstein. Crystal Structure of the Mouse P53 Core Dna-Binding Domain at 2.7 A Resolution. J.Biol.Chem. V. 276 12120 2001.
ISSN: ISSN 0021-9258
PubMed: 11152481
DOI: 10.1074/JBC.M011644200