Zinc in PDB 1hr0: Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit

The structure of Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit, PDB code: 1hr0 was solved by A.P.Carter, W.M.Clemons Jr., D.E.Brodersen, R.J.Morgan-Warren, B.T.Wimberly, V.Ramakrishnan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.90 / 3.20
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	399.581, 399.581, 176.320, 90.00, 90.00, 90.00
R / Rfree (%)	21.8 / 26.1

The structure of Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit also contains other interesting chemical elements:

Magnesium

(Mg)

65 atoms

The binding sites of Zinc atom in the Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit (pdb code 1hr0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit, PDB code: 1hr0:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit within 5.0Å range: probe atom residue distance (Å) B Occ N:Zn190 b:65.8 occ:0.92 SG N:CYS43 2.1 56.6 1.0 SG N:CYS27 2.2 44.6 1.0 SG N:CYS40 2.4 71.8 1.0 SG N:CYS24 2.4 95.1 1.0 CB N:CYS43 3.1 56.6 1.0 CB N:CYS27 3.1 44.6 1.0 CB N:CYS40 3.2 71.8 1.0 CB N:CYS24 3.4 95.1 1.0 N N:CYS27 3.7 66.6 1.0 N N:CYS40 3.7 0.2 1.0 CA N:CYS27 4.0 66.6 1.0 CA N:CYS40 4.0 0.2 1.0 N N:CYS43 4.1 74.5 1.0 CA N:CYS43 4.2 74.5 1.0 CB N:ARG29 4.2 93.5 1.0 C N:ARG26 4.7 77.1 1.0 CB N:ARG26 4.7 67.0 1.0 C N:CYS40 4.7 0.2 1.0 C N:CYS27 4.7 66.6 1.0 N N:GLY28 4.7 82.1 1.0 N2 A:G1202 4.8 79.6 1.0 O N:CYS40 4.8 0.2 1.0 N N:ARG29 4.8 0.3 1.0 CA N:CYS24 4.9 96.8 1.0 C N:LEU39 4.9 0.8 1.0 CG N:ARG26 4.9 67.0 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Initiation Factor IF1 Bound to the 30S Ribosomal Subunit within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn300 b:65.8 occ:1.64 SG D:CYS31 2.1 52.0 1.0 SG D:CYS9 2.4 62.9 1.0 SG D:CYS26 2.8 56.1 1.0 SG D:CYS12 2.8 67.5 1.0 O D:CYS31 3.5 0.6 1.0 CB D:CYS31 3.5 52.0 1.0 CA D:CYS31 3.7 0.6 1.0 O D:LEU19 3.8 0.2 1.0 CB D:CYS9 4.0 62.9 1.0 C D:CYS31 4.0 0.6 1.0 CB D:CYS12 4.0 67.5 1.0 CA D:CYS9 4.4 97.1 1.0 CB D:CYS26 4.4 56.1 1.0 CA D:CYS26 4.8 77.5 1.0

A.P.Carter, W.M.Clemons Jr., D.E.Brodersen, R.J.Morgan-Warren, T.Hartsch, B.T.Wimberly, V.Ramakrishnan. Crystal Structure of An Initiation Factor Bound to the 30S Ribosomal Subunit. Science V. 291 498 2001.
ISSN: ISSN 0036-8075
PubMed: 11228145
DOI: 10.1126/SCIENCE.1057766