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Zinc in PDB 1g2f: Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6)

Protein crystallography data

The structure of Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6), PDB code: 1g2f was solved by S.A.Wolfe, R.A.Grant, M.Elrod-Erickson, C.O.Pabo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.00
Space group P 64
Cell size a, b, c (Å), α, β, γ (°) 104.800, 104.800, 104.200, 90.00, 90.00, 120.00
R / Rfree (%) 23.3 / 26.3

Zinc Binding Sites:

The binding sites of Zinc atom in the Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6) (pdb code 1g2f). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6), PDB code: 1g2f:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in 1g2f

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Zinc binding site 1 out of 6 in the Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn301

b:50.7
occ:1.00
NE2 C:HIS129 1.9 37.9 1.0
NE2 C:HIS125 2.1 40.2 1.0
SG C:CYS107 2.3 46.6 1.0
SG C:CYS112 2.5 57.9 1.0
CD2 C:HIS129 2.8 42.6 1.0
CB C:CYS112 2.9 62.6 1.0
CE1 C:HIS129 3.0 42.6 1.0
CE1 C:HIS125 3.1 38.8 1.0
CD2 C:HIS125 3.1 41.6 1.0
CB C:CYS107 3.4 43.5 1.0
CG C:HIS129 4.0 44.4 1.0
ND1 C:HIS129 4.1 44.4 1.0
ND1 C:HIS125 4.1 39.7 1.0
CG C:HIS125 4.2 38.8 1.0
CB C:VAL109 4.4 56.8 1.0
CA C:CYS112 4.4 64.7 1.0
CG2 C:VAL109 4.5 54.8 1.0
N C:ASP113 4.7 66.4 1.0
CA C:CYS107 4.8 46.5 1.0
C C:CYS112 4.9 65.3 1.0

Zinc binding site 2 out of 6 in 1g2f

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Zinc binding site 2 out of 6 in the Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn302

b:45.5
occ:1.00
NE2 C:HIS157 2.0 44.7 1.0
NE2 C:HIS153 2.2 43.1 1.0
SG C:CYS137 2.3 38.2 1.0
SG C:CYS140 2.4 44.6 1.0
CD2 C:HIS157 2.9 43.1 1.0
CB C:CYS137 3.1 36.4 1.0
CE1 C:HIS157 3.1 44.8 1.0
CD2 C:HIS153 3.1 39.7 1.0
CE1 C:HIS153 3.2 40.3 1.0
CB C:CYS140 3.5 42.8 1.0
N C:CYS140 3.5 40.8 1.0
CA C:CYS140 4.0 40.9 1.0
CG C:HIS157 4.1 45.2 1.0
ND1 C:HIS157 4.1 44.0 1.0
CB C:ILE139 4.1 41.9 1.0
ND1 C:HIS153 4.2 37.7 1.0
CG C:HIS153 4.3 36.3 1.0
CA C:CYS137 4.5 38.4 1.0
C C:ILE139 4.6 41.6 1.0
N C:ILE139 4.7 43.2 1.0
CA C:ILE139 4.7 41.7 1.0
C C:CYS140 4.7 42.5 1.0
N C:MET141 4.8 43.7 1.0
C C:CYS137 4.8 38.6 1.0
CG1 C:ILE139 4.9 39.4 1.0
CG2 C:ILE139 4.9 37.7 1.0
CB C:ARG142 4.9 39.3 1.0
N C:ARG142 5.0 42.5 1.0
O C:CYS137 5.0 38.3 1.0

Zinc binding site 3 out of 6 in 1g2f

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Zinc binding site 3 out of 6 in the Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6) within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn303

b:47.9
occ:1.00
NE2 C:HIS185 2.1 47.1 1.0
NE2 C:HIS181 2.1 35.2 1.0
SG C:CYS168 2.2 44.0 1.0
SG C:CYS165 2.5 43.5 1.0
CE1 C:HIS185 2.9 46.5 1.0
CD2 C:HIS181 3.0 35.7 1.0
CD2 C:HIS185 3.1 49.0 1.0
CE1 C:HIS181 3.2 39.4 1.0
CB C:CYS168 3.3 45.2 1.0
CB C:CYS165 3.3 46.8 1.0
N C:CYS168 3.6 47.5 1.0
CA C:CYS168 4.0 46.1 1.0
ND1 C:HIS185 4.1 46.8 1.0
CG C:HIS181 4.2 35.9 1.0
CG C:HIS185 4.2 46.0 1.0
ND1 C:HIS181 4.2 38.9 1.0
CB C:ILE167 4.5 46.5 1.0
C C:ILE167 4.6 49.1 1.0
C C:CYS168 4.7 48.5 1.0
CA C:CYS165 4.8 47.8 1.0
N C:GLY169 4.8 50.3 1.0
CA C:ILE167 4.9 49.4 1.0
CB C:ARG170 5.0 56.3 1.0
CA C:THR182 5.0 42.1 1.0

Zinc binding site 4 out of 6 in 1g2f

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Zinc binding site 4 out of 6 in the Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6) within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn304

b:53.6
occ:1.00
NE2 F:HIS229 2.1 37.4 1.0
NE2 F:HIS225 2.1 53.5 1.0
SG F:CYS207 2.2 46.9 1.0
SG F:CYS212 2.3 63.1 1.0
CE1 F:HIS225 2.9 52.3 1.0
CB F:CYS212 3.0 69.9 1.0
CD2 F:HIS229 3.1 39.1 1.0
CE1 F:HIS229 3.1 40.8 1.0
CD2 F:HIS225 3.2 51.3 1.0
CB F:CYS207 3.3 47.9 1.0
ND1 F:HIS225 4.1 50.3 1.0
CG F:HIS225 4.2 47.6 1.0
ND1 F:HIS229 4.2 39.8 1.0
CG F:HIS229 4.2 37.6 1.0
CB F:ARG214 4.5 67.2 1.0
CB F:VAL209 4.5 52.3 1.0
CA F:CYS212 4.5 71.8 1.0
CG2 F:VAL209 4.6 51.4 1.0
CA F:CYS207 4.7 51.0 1.0
N F:ARG214 4.9 74.8 1.0
N F:ASP213 4.9 75.7 1.0

Zinc binding site 5 out of 6 in 1g2f

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Zinc binding site 5 out of 6 in the Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6) within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn305

b:34.1
occ:1.00
NE2 F:HIS253 2.0 34.6 1.0
NE2 F:HIS257 2.1 33.3 1.0
SG F:CYS240 2.3 44.3 1.0
SG F:CYS237 2.3 34.0 1.0
CD2 F:HIS257 2.9 34.7 1.0
CD2 F:HIS253 3.0 32.9 1.0
CE1 F:HIS253 3.0 31.8 1.0
CE1 F:HIS257 3.2 33.2 1.0
CB F:CYS237 3.2 31.7 1.0
CB F:CYS240 3.4 37.1 1.0
N F:CYS240 3.6 33.4 1.0
CA F:CYS240 4.0 36.4 1.0
ND1 F:HIS253 4.1 28.8 1.0
CG F:HIS257 4.1 31.6 1.0
CG F:HIS253 4.1 31.9 1.0
CB F:ILE239 4.2 36.0 1.0
ND1 F:HIS257 4.2 34.4 1.0
C F:ILE239 4.6 33.0 1.0
CA F:CYS237 4.6 33.1 1.0
C F:CYS240 4.8 37.7 1.0
CA F:ILE239 4.8 34.7 1.0
N F:ILE239 4.9 33.8 1.0
CG2 F:ILE239 4.9 30.3 1.0
CB F:ARG242 4.9 36.1 1.0
N F:MET241 5.0 38.2 1.0

Zinc binding site 6 out of 6 in 1g2f

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Zinc binding site 6 out of 6 in the Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Structure of A CYS2HIS2 Zinc Finger/Tata Box Complex (Tatazf;Clone #6) within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Zn306

b:28.9
occ:1.00
NE2 F:HIS285 2.1 39.1 1.0
NE2 F:HIS281 2.2 29.6 1.0
SG F:CYS268 2.2 31.9 1.0
SG F:CYS265 2.3 30.1 1.0
CD2 F:HIS281 3.0 26.7 1.0
CE1 F:HIS285 3.0 37.2 1.0
CD2 F:HIS285 3.1 36.9 1.0
CE1 F:HIS281 3.2 27.6 1.0
CB F:CYS265 3.3 30.5 1.0
CB F:CYS268 3.4 30.7 1.0
N F:CYS268 3.7 36.3 1.0
ND1 F:HIS285 4.1 37.6 1.0
CA F:CYS268 4.1 36.0 1.0
CG F:HIS281 4.2 27.0 1.0
CG F:HIS285 4.2 36.9 1.0
ND1 F:HIS281 4.3 31.5 1.0
CB F:ILE267 4.5 35.0 1.0
C F:ILE267 4.6 37.2 1.0
CA F:CYS265 4.7 32.0 1.0
CA F:THR282 4.8 40.0 1.0
CG2 F:THR282 4.8 35.2 1.0
CA F:ILE267 4.9 35.5 1.0
C F:CYS268 4.9 38.1 1.0
N F:ILE267 4.9 37.0 1.0
CB F:ARG270 5.0 40.5 1.0
O F:HIS281 5.0 30.6 1.0
N F:GLY269 5.0 37.2 1.0
C F:CYS265 5.0 33.1 1.0

Reference:

S.A.Wolfe, R.A.Grant, M.Elrod-Erickson, C.O.Pabo. Beyond the "Recognition Code": Structures of Two CYS2HIS2 Zinc Finger/Tata Box Complexes. Structure V. 9 717 2001.
ISSN: ISSN 0969-2126
PubMed: 11587646
DOI: 10.1016/S0969-2126(01)00632-3
Page generated: Sun Oct 13 01:16:09 2024

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