Zinc in PDB 1ew2: Crystal Structure of A Human Phosphatase

All present enzymatic activity of Crystal Structure of A Human Phosphatase:
3.1.3.1;

The structure of Crystal Structure of A Human Phosphatase, PDB code: 1ew2 was solved by M.H.Le Du, T.Stigbrand, M.J.Taussig, A.Menez, E.A.Stura, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	22.00 / 1.82
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	88.800, 114.500, 106.900, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 24.2

The structure of Crystal Structure of A Human Phosphatase also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Crystal Structure of A Human Phosphatase (pdb code 1ew2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of A Human Phosphatase, PDB code: 1ew2:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of A Human Phosphatase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Human Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1001 b:20.1 occ:1.00 NE2 A:HIS432 2.4 13.3 1.0 NE2 A:HIS320 2.4 16.8 1.0 O2 A:PO41005 2.4 20.0 1.0 OD1 A:ASP316 2.5 15.0 1.0 OD2 A:ASP316 2.6 13.9 1.0 O1 A:PO41005 2.6 25.6 1.0 CG A:ASP316 2.9 15.2 1.0 P A:PO41005 3.1 21.6 1.0 CD2 A:HIS432 3.2 13.8 1.0 CD2 A:HIS320 3.2 16.8 1.0 CE1 A:HIS320 3.3 14.2 1.0 CE1 A:HIS432 3.3 15.1 1.0 O A:HOH1320 3.9 36.9 1.0 O4 A:PO41005 3.9 18.6 1.0 O A:HOH1076 4.0 15.5 1.0 ZN A:ZN1002 4.0 19.2 1.0 O3 A:PO41005 4.0 21.6 1.0 O A:HOH1377 4.1 32.3 1.0 NE2 A:HIS358 4.2 17.4 1.0 CB A:ASP316 4.3 14.2 1.0 CG A:HIS432 4.3 13.8 1.0 ND1 A:HIS432 4.4 14.0 1.0 ND1 A:HIS320 4.4 14.0 1.0 CE1 A:HIS360 4.4 12.0 1.0 CG A:HIS320 4.4 15.4 1.0 NE2 A:HIS360 4.5 11.2 1.0 OD1 A:ASP42 4.6 13.3 1.0 CE1 A:HIS358 4.7 13.9 1.0 O A:ASP316 5.0 12.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of A Human Phosphatase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Human Phosphatase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1002 b:19.2 occ:1.00 O2 A:PO41005 2.3 20.0 1.0 OD1 A:ASP42 2.3 13.3 1.0 OD2 A:ASP357 2.4 17.7 1.0 CE1 A:HIS358 2.5 13.9 1.0 OG A:SER92 2.6 23.3 1.0 OD2 A:ASP42 2.7 13.3 1.0 CG A:ASP42 2.9 13.7 1.0 NE2 A:HIS358 2.9 17.4 1.0 CG A:ASP357 3.2 14.8 1.0 OD1 A:ASP357 3.2 13.5 1.0 P A:PO41005 3.4 21.6 1.0 ND1 A:HIS358 3.4 17.3 1.0 OD2 A:ASP316 3.7 13.9 1.0 CB A:SER92 3.8 19.1 1.0 O4 A:PO41005 3.9 18.6 1.0 O3 A:PO41005 4.0 21.6 1.0 ZN A:ZN1001 4.0 20.1 1.0 CG A:ASP316 4.0 15.2 1.0 CA A:SER92 4.1 17.3 1.0 CD2 A:HIS358 4.1 12.5 1.0 CE1 A:HIS432 4.2 15.1 1.0 N A:SER92 4.2 15.8 1.0 N A:GLY43 4.3 10.6 1.0 NE2 A:HIS432 4.3 13.3 1.0 CB A:ASP42 4.3 12.8 1.0 CG A:HIS358 4.4 13.3 1.0 CB A:ASP357 4.5 13.3 1.0 OD1 A:ASP316 4.5 15.0 1.0 O A:HOH1097 4.5 13.8 1.0 CA A:ASP42 4.6 10.8 1.0 C A:ASP42 4.6 12.0 1.0 O1 A:PO41005 4.6 25.6 1.0 CB A:ASP316 4.7 14.2 1.0 O A:HOH1115 4.7 14.3 1.0 O A:HOH1076 4.7 15.5 1.0 MG A:MG1003 4.8 14.5 1.0 CA A:GLY43 4.9 12.8 1.0 C A:ASP91 5.0 16.7 1.0 ND1 A:HIS432 5.0 14.0 1.0

M.H.Le Du, T.Stigbrand, M.J.Taussig, A.Menez, E.A.Stura. Crystal Structure of Alkaline Phosphatase From Human Placenta at 1.8 A Resolution. Implication For A Substrate Specificity. J.Biol.Chem. V. 276 9158 2001.
ISSN: ISSN 0021-9258
PubMed: 11124260
DOI: 10.1074/JBC.M009250200