Zinc in PDB 1en7: Endonuclease VII (Endovii) From Phage T4

All present enzymatic activity of Endonuclease VII (Endovii) From Phage T4:
3.1.22.4;

The structure of Endonuclease VII (Endovii) From Phage T4, PDB code: 1en7 was solved by H.Raaijmakers, O.Vix, I.Toro, D.Suck, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	144.990, 39.450, 75.750, 90.00, 106.20, 90.00
R / Rfree (%)	23.9 / 30.8

The structure of Endonuclease VII (Endovii) From Phage T4 also contains other interesting chemical elements:

Calcium

(Ca)

3 atoms

The binding sites of Zinc atom in the Endonuclease VII (Endovii) From Phage T4 (pdb code 1en7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Endonuclease VII (Endovii) From Phage T4, PDB code: 1en7:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Endonuclease VII (Endovii) From Phage T4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Endonuclease VII (Endovii) From Phage T4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:37.5 occ:1.00 SG A:CYS26 2.3 30.4 1.0 SG A:CYS23 2.3 31.6 1.0 SG A:CYS58 2.4 35.2 1.0 SG A:CYS61 2.5 31.6 1.0 CB A:CYS61 3.1 32.4 1.0 CB A:CYS23 3.2 34.0 1.0 CB A:CYS26 3.2 31.9 1.0 N A:CYS26 3.6 32.4 1.0 CB A:CYS58 3.6 34.8 1.0 CA A:CYS26 4.0 33.4 1.0 N A:CYS58 4.1 35.0 1.0 CA A:CYS61 4.3 33.9 1.0 N A:CYS61 4.3 35.0 1.0 CA A:CYS58 4.4 35.7 1.0 CA A:CYS23 4.6 33.5 1.0 CB A:ILE25 4.7 34.5 1.0 C A:ILE25 4.7 33.5 1.0 C A:CYS26 4.8 34.5 1.0 N A:GLN27 4.9 34.1 1.0 O A:CYS58 4.9 39.5 1.0 C A:CYS58 5.0 37.2 1.0 N A:ILE25 5.0 36.9 1.0

Zinc binding site 2 out of 2 in the Endonuclease VII (Endovii) From Phage T4


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Endonuclease VII (Endovii) From Phage T4 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn402 b:34.5 occ:1.00 SG B:CYS23 2.3 28.2 1.0 SG B:CYS58 2.3 26.7 1.0 SG B:CYS26 2.3 29.5 1.0 SG B:CYS61 2.5 26.1 1.0 CB B:CYS23 3.1 25.5 1.0 CB B:CYS26 3.3 30.5 1.0 CB B:CYS61 3.3 26.7 1.0 CB B:CYS58 3.7 27.3 1.0 N B:CYS26 3.7 31.6 1.0 N B:CYS58 3.9 24.9 1.0 CA B:CYS26 4.1 31.4 1.0 N B:CYS61 4.3 26.6 1.0 CA B:CYS58 4.3 25.1 1.0 CA B:CYS61 4.3 27.9 1.0 CA B:CYS23 4.5 27.3 1.0 CB B:ILE25 4.8 30.8 1.0 C B:ILE25 4.8 33.6 1.0 C B:CYS26 4.9 34.8 1.0 C B:CYS58 4.9 22.9 1.0 N B:ILE25 4.9 29.4 1.0 O B:CYS58 5.0 24.6 1.0 NH2 B:ARG28 5.0 42.4 1.0

H.Raaijmakers, O.Vix, I.Toro, S.Golz, B.Kemper, D.Suck. X-Ray Structure of T4 Endonuclease VII: A Dna Junction Resolvase with A Novel Fold and Unusual Domain-Swapped Dimer Architecture. Embo J. V. 18 1447 1999.
ISSN: ISSN 0261-4189
PubMed: 10075917
DOI: 10.1093/EMBOJ/18.6.1447