Atomistry » Zinc » PDB 1ed8-1evl » 1ee2
Atomistry »
  Zinc »
    PDB 1ed8-1evl »
      1ee2 »

Zinc in PDB 1ee2: The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution

Enzymatic activity of The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution

All present enzymatic activity of The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution:
1.1.1.1;

Protein crystallography data

The structure of The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution, PDB code: 1ee2 was solved by H.W.Adolph, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 1.54
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 55.030, 73.160, 92.490, 90.00, 102.48, 90.00
R / Rfree (%) 14.5 / 18.3

Zinc Binding Sites:

The binding sites of Zinc atom in the The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution (pdb code 1ee2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution, PDB code: 1ee2:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 1ee2

Go back to Zinc Binding Sites List in 1ee2
Zinc binding site 1 out of 4 in the The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1300

b:10.4
occ:1.00
O26 A:CHD1150 2.0 12.8 1.0
NE2 A:HIS67 2.0 9.7 1.0
SG A:CYS173 2.3 9.3 1.0
SG A:CYS46 2.4 8.9 1.0
CE1 A:HIS67 3.0 10.7 1.0
CD2 A:HIS67 3.1 10.8 1.0
C24 A:CHD1150 3.1 24.6 1.0
CB A:CYS46 3.3 9.5 1.0
CB A:CYS173 3.4 9.8 1.0
C5N A:NAD1100 3.5 7.8 1.0
C23 A:CHD1150 3.9 28.5 1.0
C4N A:NAD1100 4.0 8.2 1.0
C6N A:NAD1100 4.1 9.8 1.0
ND1 A:HIS67 4.2 10.2 1.0
CG A:HIS67 4.2 10.2 1.0
O25 A:CHD1150 4.2 21.7 1.0
CB A:SER48 4.2 11.9 1.0
OG A:SER48 4.2 16.1 1.0
NH2 A:ARG368 4.6 10.7 1.0
CA A:CYS46 4.8 8.2 1.0
CA A:CYS173 4.8 9.4 1.0
CE2 A:PHE93 4.8 11.6 1.0
N A:SER48 5.0 8.9 1.0
OE2 A:GLU68 5.0 12.9 1.0

Zinc binding site 2 out of 4 in 1ee2

Go back to Zinc Binding Sites List in 1ee2
Zinc binding site 2 out of 4 in the The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1301

b:12.3
occ:1.00
SG A:CYS100 2.3 11.5 1.0
SG A:CYS111 2.4 9.9 1.0
SG A:CYS97 2.4 11.5 1.0
SG A:CYS103 2.4 10.7 1.0
CB A:CYS103 3.4 14.4 1.0
CB A:CYS111 3.4 10.8 1.0
CB A:CYS100 3.4 13.0 1.0
CB A:CYS97 3.4 11.9 1.0
N A:CYS97 3.5 10.0 1.0
CA A:CYS111 3.8 9.1 1.0
N A:CYS100 3.8 15.4 1.0
CA A:CYS97 3.9 11.3 1.0
N A:GLY98 3.9 12.8 1.0
N A:LEU112 4.0 11.9 1.0
CA A:CYS100 4.1 15.6 1.0
C A:CYS111 4.2 12.6 1.0
N A:CYS103 4.3 11.8 1.0
C A:CYS97 4.3 14.7 1.0
CA A:CYS103 4.4 11.7 1.0
N A:LYS99 4.5 15.1 1.0
C A:GLN96 4.6 12.4 1.0
CG A:LYS113 4.7 22.2 1.0
N A:LYS113 4.7 12.5 1.0
C A:CYS100 4.8 13.7 1.0
O A:CYS100 4.9 15.2 1.0
CA A:GLN96 5.0 10.7 1.0
C A:LYS99 5.0 18.2 1.0

Zinc binding site 3 out of 4 in 1ee2

Go back to Zinc Binding Sites List in 1ee2
Zinc binding site 3 out of 4 in the The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1302

b:11.5
occ:1.00
NE2 B:HIS67 2.1 12.2 1.0
O25 B:CHD1250 2.1 12.1 1.0
SG B:CYS173 2.2 10.2 1.0
SG B:CYS46 2.4 10.3 1.0
CE1 B:HIS67 3.0 13.8 1.0
CD2 B:HIS67 3.1 11.0 1.0
C24 B:CHD1250 3.3 24.5 1.0
CB B:CYS46 3.4 10.8 1.0
CB B:CYS173 3.4 8.6 1.0
C5N B:NAD1200 3.5 8.7 1.0
C23 B:CHD1250 3.9 28.1 1.0
C4N B:NAD1200 4.0 9.5 1.0
C6N B:NAD1200 4.1 8.7 1.0
ND1 B:HIS67 4.2 11.3 1.0
CG B:HIS67 4.2 9.6 1.0
CB B:SER48 4.2 11.7 1.0
O26 B:CHD1250 4.2 21.9 1.0
OG B:SER48 4.3 16.1 1.0
NH2 B:ARG368 4.7 13.0 1.0
CA B:CYS173 4.8 10.3 1.0
CE2 B:PHE93 4.8 12.7 1.0
CA B:CYS46 4.8 10.6 1.0
OE2 B:GLU68 4.9 15.7 1.0
N B:SER48 5.0 8.9 1.0

Zinc binding site 4 out of 4 in 1ee2

Go back to Zinc Binding Sites List in 1ee2
Zinc binding site 4 out of 4 in the The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of The Structure of Steroid-Active Alcohol Dehydrogenase at 1.54 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1303

b:11.5
occ:1.00
SG B:CYS111 2.3 10.5 1.0
SG B:CYS100 2.3 9.6 1.0
SG B:CYS97 2.4 11.1 1.0
SG B:CYS103 2.4 10.1 1.0
CB B:CYS111 3.3 10.0 1.0
CB B:CYS97 3.4 12.0 1.0
CB B:CYS100 3.4 12.1 1.0
CB B:CYS103 3.4 10.1 1.0
N B:CYS97 3.5 11.7 1.0
CA B:CYS111 3.7 8.9 1.0
N B:CYS100 3.8 13.5 1.0
CA B:CYS97 3.9 13.1 1.0
N B:GLY98 3.9 11.8 1.0
N B:LEU112 4.0 11.6 1.0
C B:CYS111 4.1 11.2 1.0
CA B:CYS100 4.2 14.5 1.0
N B:CYS103 4.2 12.8 1.0
C B:CYS97 4.3 14.0 1.0
CA B:CYS103 4.4 11.8 1.0
N B:LYS99 4.5 15.0 1.0
C B:GLN96 4.6 13.6 1.0
N B:LYS113 4.8 11.2 1.0
NZ B:LYS113 4.8 33.2 0.5
CG B:LYS113 4.8 17.1 1.0
C B:CYS100 4.9 13.4 1.0
CA B:GLN96 4.9 9.9 1.0
CA B:GLY98 5.0 13.4 1.0
O B:CYS100 5.0 14.0 1.0
C B:LYS99 5.0 19.1 1.0

Reference:

H.W.Adolph, P.Zwart, R.Meijers, I.Hubatsch, M.Kiefer, V.Lamzin, E.Cedergren-Zeppezauer. Structural Basis For Substrate Specificity Differences of Horse Liver Alcohol Dehydrogenase Isozymes. Biochemistry V. 39 12885 2000.
ISSN: ISSN 0006-2960
PubMed: 11041853
DOI: 10.1021/BI001376S
Page generated: Sun Oct 13 00:09:46 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy