Zinc in PDB 1b6z: 6-Pyruvoyl Tetrahydropterin Synthase

All present enzymatic activity of 6-Pyruvoyl Tetrahydropterin Synthase:
4.6.1.10;

The structure of 6-Pyruvoyl Tetrahydropterin Synthase, PDB code: 1b6z was solved by T.Ploom, B.Thoeny, J.Yim, S.Lee, H.Nar, W.Leimbacher, R.Huber, J.Richardson, G.Auerbach, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.00
Space group	P 3 2 1
Cell size a, b, c (Å), α, β, γ (°)	121.500, 121.500, 61.250, 90.00, 90.00, 120.00
R / Rfree (%)	21.6 / 25.6

The binding sites of Zinc atom in the 6-Pyruvoyl Tetrahydropterin Synthase (pdb code 1b6z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the 6-Pyruvoyl Tetrahydropterin Synthase, PDB code: 1b6z:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the 6-Pyruvoyl Tetrahydropterin Synthase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of 6-Pyruvoyl Tetrahydropterin Synthase within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:32.0 occ:1.00 NE2 A:HIS50 2.3 22.3 1.0 NE2 A:HIS23 2.3 20.1 1.0 NE2 A:HIS48 2.3 34.2 1.0 O A:HOH469 2.4 37.9 1.0 CD2 A:HIS48 3.1 33.4 1.0 CE1 A:HIS23 3.1 21.0 1.0 CD2 A:HIS50 3.1 20.6 1.0 CE1 A:HIS50 3.3 20.2 1.0 OE2 A:GLU133 3.4 31.6 1.0 CE1 A:HIS48 3.4 34.1 1.0 CD2 A:HIS23 3.4 23.5 1.0 CD A:GLU133 3.9 29.3 1.0 CG A:HIS48 4.3 33.5 1.0 ND1 A:HIS23 4.3 24.0 1.0 OE1 A:GLU133 4.3 27.4 1.0 CG A:HIS50 4.3 21.6 1.0 ND1 A:HIS50 4.4 22.5 1.0 ND1 A:HIS48 4.4 34.3 1.0 CG A:HIS23 4.5 22.7 1.0 SG A:CYS42 4.6 32.1 1.0 CG A:GLU133 4.6 27.0 1.0 OH A:TYR52 4.8 20.2 1.0

Zinc binding site 2 out of 2 in the 6-Pyruvoyl Tetrahydropterin Synthase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of 6-Pyruvoyl Tetrahydropterin Synthase within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn402 b:32.0 occ:1.00 NE2 B:HIS23 2.2 24.5 1.0 NE2 B:HIS50 2.3 21.8 1.0 NE2 B:HIS48 2.3 30.7 1.0 O B:HOH457 2.5 45.1 1.0 OE2 B:GLU133 3.2 36.2 1.0 CE1 B:HIS23 3.2 25.0 1.0 CE1 B:HIS50 3.2 20.4 1.0 CD2 B:HIS48 3.3 27.9 1.0 CE1 B:HIS48 3.3 31.1 1.0 CD2 B:HIS23 3.3 26.5 1.0 CD2 B:HIS50 3.3 21.9 1.0 CD B:GLU133 3.9 34.1 1.0 ND1 B:HIS23 4.3 27.7 1.0 ND1 B:HIS50 4.4 23.3 1.0 ND1 B:HIS48 4.4 31.1 1.0 OE1 B:GLU133 4.4 33.9 1.0 CG B:HIS48 4.4 29.7 1.0 CG B:HIS23 4.4 26.5 1.0 CG B:HIS50 4.4 20.5 1.0 CG B:GLU133 4.7 30.3 1.0 O B:HOH405 4.8 21.4 1.0 OH B:TYR52 4.8 18.9 1.0 O B:HOH424 4.9 68.2 1.0 SG B:CYS42 4.9 40.5 1.0

T.Ploom, B.Thony, J.Yim, S.Lee, H.Nar, W.Leimbacher, J.Richardson, R.Huber, G.Auerbach. Crystallographic and Kinetic Investigations on the Mechanism of 6-Pyruvoyl Tetrahydropterin Synthase. J.Mol.Biol. V. 286 851 1999.
ISSN: ISSN 0022-2836
PubMed: 10024455
DOI: 10.1006/JMBI.1998.2511