Zinc in PDB 9dnr: Structure of UBR2-Rwf Complex

The structure of Structure of UBR2-Rwf Complex, PDB code: 9dnr was solved by S.Huang, J.Wu, S.Taylor, Y.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	27.91 / 1.22
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	29.235, 36.957, 29.615, 90, 109.56, 90
R / Rfree (%)	16.8 / 18

The binding sites of Zinc atom in the Structure of UBR2-Rwf Complex (pdb code 9dnr). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Structure of UBR2-Rwf Complex, PDB code: 9dnr:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Structure of UBR2-Rwf Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of UBR2-Rwf Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn201 b:4.1 occ:1.00 SG A:CYS99 2.3 4.8 1.0 SG A:CYS124 2.3 3.8 1.0 SG A:CYS149 2.3 4.1 1.0 SG A:CYS127 2.4 4.1 1.0 CB A:CYS149 3.2 4.3 1.0 CB A:CYS99 3.2 6.5 1.0 CB A:CYS127 3.3 4.3 1.0 CB A:CYS124 3.5 4.3 1.0 N A:CYS124 3.9 4.6 1.0 CB A:CYS151 4.2 4.8 1.0 CA A:CYS124 4.3 5.1 1.0 N A:CYS127 4.3 4.7 1.0 ZN A:ZN202 4.4 5.0 1.0 CA A:CYS127 4.4 4.3 1.0 CB A:ARG101 4.6 7.0 1.0 O A:HOH367 4.6 17.0 1.0 CA A:CYS149 4.6 3.9 1.0 CA A:CYS99 4.6 8.4 1.0 CE1 A:HIS166 4.7 6.2 1.0 C A:CYS124 4.8 4.2 1.0 O A:CYS124 4.9 4.2 1.0 N A:ARG101 5.0 6.6 1.0

Zinc binding site 2 out of 3 in the Structure of UBR2-Rwf Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of UBR2-Rwf Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn202 b:5.0 occ:1.00 ND1 A:HIS166 2.1 6.1 1.0 SG A:CYS151 2.3 6.2 1.0 SG A:CYS163 2.3 4.8 1.0 SG A:CYS127 2.3 4.1 1.0 CE1 A:HIS166 3.0 6.2 1.0 CG A:HIS166 3.1 7.5 1.0 CB A:CYS151 3.2 4.8 1.0 CB A:CYS127 3.2 4.3 1.0 CB A:CYS163 3.4 6.2 1.0 CB A:HIS166 3.5 7.9 1.0 CA A:CYS127 3.9 4.3 1.0 N A:CYS163 4.1 7.7 1.0 NE2 A:HIS166 4.1 8.3 1.0 CD2 A:HIS166 4.2 9.2 1.0 O A:HOH322 4.3 8.3 1.0 CB A:CYS99 4.3 6.5 1.0 CA A:CYS163 4.3 7.3 1.0 N A:HIS166 4.3 7.8 1.0 ZN A:ZN201 4.4 4.1 1.0 CA A:HIS166 4.6 8.5 1.0 CA A:CYS151 4.7 5.5 1.0 N A:CYS127 4.8 4.7 1.0 C A:CYS163 4.9 7.9 1.0 CB A:LYS165 5.0 13.0 1.0

Zinc binding site 3 out of 3 in the Structure of UBR2-Rwf Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of UBR2-Rwf Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn203 b:6.5 occ:1.00 ND1 A:HIS133 2.1 6.6 1.0 ND1 A:HIS136 2.1 6.5 1.0 SG A:CYS115 2.3 8.0 1.0 SG A:CYS112 2.3 6.9 1.0 CE1 A:HIS133 2.9 5.3 1.0 CE1 A:HIS136 3.0 8.5 1.0 CG A:HIS133 3.1 5.2 1.0 CB A:CYS115 3.1 7.0 1.0 CG A:HIS136 3.2 7.7 1.0 CB A:CYS112 3.3 7.1 1.0 CB A:HIS136 3.6 8.5 1.0 CB A:HIS133 3.6 6.7 1.0 CA A:HIS133 3.8 5.8 1.0 N A:CYS115 3.9 9.4 1.0 NE2 A:HIS133 4.1 6.3 1.0 NE2 A:HIS136 4.1 9.0 1.0 CA A:CYS115 4.1 8.8 1.0 CD2 A:HIS133 4.2 5.5 1.0 CD2 A:HIS136 4.2 10.0 1.0 CB A:ASP114 4.3 9.5 1.0 N A:HIS133 4.6 6.5 1.0 C A:ASP114 4.6 10.3 1.0 CG2 A:ILE132 4.7 8.1 1.0 CA A:CYS112 4.7 7.4 1.0 CA A:ASP114 4.9 10.3 1.0 C A:HIS133 5.0 6.8 1.0 N A:ASP114 5.0 9.9 1.0

S.Huang, J.Wu, S.Taylor, Y.Chen. Structural Flexibility and Selectivity of N-End Rule Ligases To Be Published.