Zinc in PDB 8vyo: Cryo-Em Structure of the Braf Wt Monomer

All present enzymatic activity of Cryo-Em Structure of the Braf Wt Monomer:
2.7.11.1;

The binding sites of Zinc atom in the Cryo-Em Structure of the Braf Wt Monomer (pdb code 8vyo). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of the Braf Wt Monomer, PDB code: 8vyo:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of the Braf Wt Monomer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of the Braf Wt Monomer within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn901 b:116.2 occ:1.00 HB3 A:CYS248 1.6 89.5 1.0 CB A:CYS248 2.1 89.5 1.0 HB2 A:CYS248 2.3 89.5 1.0 ND1 A:HIS269 2.3 88.5 1.0 SG A:CYS248 2.3 89.5 1.0 SG A:CYS251 2.3 97.2 1.0 HG A:CYS251 2.6 97.2 1.0 CE1 A:HIS269 2.7 88.5 1.0 HE1 A:HIS269 2.8 88.5 1.0 CG A:HIS269 3.2 88.5 1.0 HG A:CYS248 3.3 89.5 1.0 HB2 A:LYS253 3.4 90.5 1.0 CA A:CYS248 3.5 89.5 1.0 NE2 A:HIS269 3.6 88.5 1.0 HB2 A:HIS269 3.6 88.5 1.0 H A:LYS253 3.7 90.5 1.0 HA A:HIS269 3.7 88.5 1.0 CD2 A:HIS269 3.8 88.5 1.0 CB A:HIS269 3.9 88.5 1.0 H A:CYS251 3.9 97.2 1.0 H A:CYS248 3.9 89.5 1.0 O A:CYS248 4.0 89.5 1.0 CB A:CYS251 4.0 97.2 1.0 O A:LYS253 4.1 90.5 1.0 C A:CYS248 4.1 89.5 1.0 HA A:CYS248 4.1 89.5 1.0 HD11 A:LEU255 4.2 79.2 1.0 HE2 A:HIS269 4.2 88.5 1.0 N A:CYS248 4.2 89.5 1.0 CB A:LYS253 4.3 90.5 1.0 HB2 A:LEU255 4.3 79.2 1.0 CA A:HIS269 4.3 88.5 1.0 HB3 A:CYS251 4.3 97.2 1.0 N A:LYS253 4.4 90.5 1.0 HG3 A:LYS253 4.5 90.5 1.0 N A:CYS251 4.5 97.2 1.0 HB2 A:CYS251 4.6 97.2 1.0 C A:LYS253 4.6 90.5 1.0 H A:ARG252 4.6 104.0 1.0 HD2 A:HIS269 4.6 88.5 1.0 CA A:LYS253 4.6 90.5 1.0 CA A:CYS251 4.7 97.2 1.0 HB3 A:HIS269 4.7 88.5 1.0 HB3 A:PHE250 4.7 92.6 1.0 O A:LEU254 4.8 78.9 1.0 N A:ARG252 4.8 104.0 1.0 C A:CYS251 4.9 97.2 1.0 H A:PHE250 4.9 92.6 1.0 HB3 A:LYS253 4.9 90.5 1.0 CG A:LYS253 4.9 90.5 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of the Braf Wt Monomer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of the Braf Wt Monomer within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn902 b:116.3 occ:1.00 HA A:HIS235 1.6 92.0 1.0 SG A:CYS261 2.3 94.5 1.0 SG A:CYS264 2.3 97.5 1.0 ND1 A:HIS235 2.3 92.0 1.0 HG A:CYS261 2.4 94.5 1.0 OG1 A:THR263 2.4 90.8 1.0 CA A:HIS235 2.4 92.0 1.0 HG1 A:THR263 2.6 90.8 1.0 CG A:HIS235 2.6 92.0 1.0 CB A:HIS235 2.7 92.0 1.0 HB3 A:HIS235 2.8 92.0 1.0 H A:ASN236 2.8 88.8 1.0 HG A:CYS264 3.1 97.5 1.0 CE1 A:HIS235 3.3 92.0 1.0 N A:HIS235 3.4 92.0 1.0 N A:ASN236 3.4 88.8 1.0 C A:HIS235 3.4 92.0 1.0 HB2 A:CYS264 3.6 97.5 1.0 CB A:CYS264 3.7 97.5 1.0 HB2 A:HIS235 3.7 92.0 1.0 CD2 A:HIS235 3.7 92.0 1.0 CB A:THR263 3.7 90.8 1.0 H A:THR263 3.7 90.8 1.0 HE1 A:HIS235 3.8 92.0 1.0 HB A:THR263 3.9 90.8 1.0 NE2 A:HIS235 4.0 92.0 1.0 CB A:CYS261 4.1 94.5 1.0 N A:CYS264 4.1 97.5 1.0 H A:CYS264 4.1 97.5 1.0 H A:HIS235 4.2 92.0 1.0 OD1 A:ASN236 4.3 88.8 1.0 C A:THR263 4.3 90.8 1.0 HB3 A:CYS264 4.3 97.5 1.0 CA A:THR263 4.3 90.8 1.0 N A:THR263 4.4 90.8 1.0 HD2 A:HIS235 4.4 92.0 1.0 HB2 A:CYS261 4.4 94.5 1.0 HB3 A:CYS261 4.4 94.5 1.0 O A:ASN236 4.5 88.8 1.0 CA A:CYS264 4.5 97.5 1.0 O A:HIS235 4.6 92.0 1.0 HG21 A:THR263 4.7 90.8 1.0 CG2 A:THR263 4.7 90.8 1.0 CA A:ASN236 4.8 88.8 1.0 HE2 A:HIS235 4.8 92.0 1.0 HG23 A:THR263 4.9 90.8 1.0 O A:THR263 4.9 90.8 1.0

H.Lavoie, T.Jin, D.Lajoie, M.Decossas, P.Gendron, B.Wang, F.Filandr, M.Sahmi, C.Hwa Jo, S.Weber, G.Arseneault, S.Tripathy, P.Beaulieu, D.A.Schuetz, D.C.Schriemer, A.Marinier, W.J.Rice, P.Maisonneuve, M.Therrien. Braf Oncogenic Mutants Evade Autoinhibition Through A Common Mechanism Science 2025.
ISSN: ESSN 1095-9203
DOI: 10.1126/SCIENCE.ADP2742