Zinc in PDB 6tut: Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex

All present enzymatic activity of Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex:
2.7.7.6;

The binding sites of Zinc atom in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex (pdb code 6tut). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 7 binding sites of Zinc where determined in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex, PDB code: 6tut:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7;

Zinc binding site 1 out of 7 in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2000 b:74.1 occ:1.00 NE2 A:HIS80 2.2 42.8 1.0 SG A:CYS77 2.4 51.8 1.0 SG A:CYS67 2.4 64.4 1.0 SG A:CYS70 2.4 59.0 1.0 CD2 A:HIS80 2.4 42.8 1.0 CB A:CYS67 3.1 64.4 1.0 CE1 A:HIS80 3.4 42.8 1.0 CB A:CYS77 3.7 51.8 1.0 CB A:CYS70 3.7 59.0 1.0 CG A:HIS80 3.7 42.8 1.0 N A:CYS70 3.9 59.0 1.0 ND1 A:HIS80 4.2 42.8 1.0 CA A:CYS77 4.4 51.8 1.0 CA A:CYS70 4.4 59.0 1.0 N A:GLY79 4.4 41.0 1.0 CB A:THR69 4.6 57.8 1.0 CA A:GLY59 4.6 55.3 1.0 CA A:CYS67 4.6 64.4 1.0 N A:HIS78 4.7 41.4 1.0 CA A:GLY79 4.8 41.0 1.0 N A:THR69 4.8 57.8 1.0 N A:GLY59 4.8 55.3 1.0 C A:CYS77 4.9 51.8 1.0 C A:THR69 4.9 57.8 1.0 CB A:HIS80 4.9 42.8 1.0 N A:HIS71 5.0 63.6 1.0 C A:GLY79 5.0 41.0 1.0

Zinc binding site 2 out of 7 in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2001 b:0.2 occ:1.00 SG A:CYS107 2.4 65.6 1.0 SG A:CYS157 2.4 98.8 1.0 SG A:CYS154 2.5 97.9 1.0 CB A:CYS154 2.8 97.9 1.0 N A:CYS157 2.9 98.8 1.0 SG A:CYS110 3.0 69.2 1.0 CB A:CYS157 3.4 98.8 1.0 CA A:CYS157 3.5 98.8 1.0 O A:CYS157 3.6 98.8 1.0 CB A:CYS107 3.7 65.6 1.0 C A:HIS156 3.8 94.3 1.0 CB A:HIS156 3.9 94.3 1.0 C A:CYS157 3.9 98.8 1.0 N A:HIS156 4.0 94.3 1.0 CA A:HIS156 4.1 94.3 1.0 CA A:CYS154 4.3 97.9 1.0 CB A:CYS110 4.3 69.2 1.0 N A:CYS110 4.6 69.2 1.0 C A:CYS154 4.8 97.9 1.0 O A:HIS156 4.8 94.3 1.0 N A:LEU155 4.9 94.3 1.0 CG A:HIS156 4.9 94.3 1.0

Zinc binding site 3 out of 7 in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1201 b:58.2 occ:1.00 SG B:CYS1098 2.4 37.3 1.0 SG B:CYS1107 2.4 43.2 1.0 SG B:CYS1095 2.4 36.7 1.0 SG B:CYS1110 2.7 43.1 1.0 CB B:CYS1098 2.9 37.3 1.0 CB B:CYS1095 3.1 36.7 1.0 CB B:CYS1107 3.6 43.2 1.0 CG2 B:THR1109 3.7 37.8 1.0 N B:CYS1098 3.9 37.3 1.0 CA B:CYS1098 4.0 37.3 1.0 CB B:CYS1110 4.1 43.1 1.0 CG B:LYS1097 4.2 37.6 1.0 N B:CYS1110 4.2 43.1 1.0 CA B:CYS1095 4.5 36.7 1.0 CA B:CYS1110 4.8 43.1 1.0 O B:CYS1110 4.9 43.1 1.0 C B:THR1109 4.9 37.8 1.0 C B:LYS1097 5.0 37.6 1.0 CD B:LYS1097 5.0 37.6 1.0 CA B:CYS1107 5.0 43.2 1.0

Zinc binding site 4 out of 7 in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ I:Zn201 b:1.0 occ:1.00 SG I:CYS8 2.5 95.6 1.0 SG I:CYS26 2.5 0.8 1.0 SG I:CYS5 2.5 97.6 1.0 CB I:CYS29 2.6 1.0 1.0 SG I:CYS29 2.7 1.0 1.0 CB I:CYS26 2.8 0.8 1.0 CB I:CYS5 3.8 97.6 1.0 CB I:CYS8 3.9 95.6 1.0 CA I:CYS29 4.0 1.0 1.0 O I:PRO6 4.2 97.3 1.0 CA I:CYS26 4.3 0.8 1.0 N I:CYS8 4.4 95.6 1.0 CB I:TYR31 4.4 0.9 1.0 O I:CYS29 4.5 1.0 1.0 C I:CYS29 4.5 1.0 1.0 CA I:CYS8 4.6 95.6 1.0 N I:CYS29 4.6 1.0 1.0 N I:ASN9 4.8 87.9 1.0 N I:ASN10 4.9 88.1 1.0 CB I:ASN10 4.9 88.1 1.0 C I:CYS8 4.9 95.6 1.0 C I:CYS26 4.9 0.8 1.0 O I:ARG27 5.0 1.0 1.0 ND2 I:ASN10 5.0 88.1 1.0 OG I:SER7 5.0 97.6 1.0

Zinc binding site 5 out of 7 in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn100 b:36.3 occ:1.00 SG J:CYS46 2.4 14.5 1.0 SG J:CYS7 2.5 11.4 1.0 SG J:CYS45 2.5 14.5 1.0 SG J:CYS10 2.7 14.0 1.0 CB J:CYS10 2.7 14.0 1.0 CB J:CYS7 3.3 11.4 1.0 N J:CYS10 3.3 14.0 1.0 CA J:CYS10 3.6 14.0 1.0 CB J:CYS45 3.7 14.5 1.0 N J:CYS46 3.9 14.5 1.0 CB J:CYS46 3.9 14.5 1.0 NE J:ARG43 4.0 14.3 1.0 C J:SER9 4.0 11.9 1.0 C J:CYS45 4.1 14.5 1.0 CA J:CYS46 4.2 14.5 1.0 NH2 J:ARG43 4.2 14.3 1.0 O J:CYS45 4.5 14.5 1.0 CA J:CYS45 4.5 14.5 1.0 CB J:SER9 4.6 11.9 1.0 N J:SER9 4.6 11.9 1.0 C J:CYS10 4.6 14.0 1.0 CA J:SER9 4.6 11.9 1.0 CZ J:ARG43 4.6 14.3 1.0 CA J:CYS7 4.7 11.4 1.0 N J:GLY11 4.7 12.8 1.0 O J:SER9 4.7 11.9 1.0 CB J:LYS12 4.7 11.8 1.0 CB J:ARG43 4.8 14.3 1.0 N J:LYS12 4.9 11.8 1.0 C J:CYS7 5.0 11.4 1.0 CD J:ARG43 5.0 14.3 1.0

Zinc binding site 6 out of 7 in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn101 b:63.0 occ:1.00 O J:ARG6 2.1 12.5 1.0 N J:ARG6 2.3 12.5 1.0 CA J:ARG6 2.5 12.5 1.0 C J:ARG6 2.5 12.5 1.0 CB J:ARG6 2.5 12.5 1.0 C J:VAL5 3.5 11.9 1.0 CG J:ARG6 3.6 12.5 1.0 N J:CYS7 3.7 11.4 1.0 CB B:ARG780 3.8 11.3 1.0 N J:VAL5 3.8 11.9 1.0 CD2 C:LEU103 4.0 13.6 1.0 O B:ARG780 4.0 11.3 1.0 CA J:VAL5 4.1 11.9 1.0 CD B:ARG780 4.2 11.3 1.0 CG B:ARG780 4.2 11.3 1.0 CB J:PRO4 4.3 11.5 1.0 O J:CYS7 4.3 11.4 1.0 C J:CYS7 4.3 11.4 1.0 CD J:ARG6 4.3 12.5 1.0 C J:PRO4 4.4 11.5 1.0 CD1 J:PHE8 4.4 11.2 1.0 CE1 J:PHE8 4.5 11.2 1.0 CB J:VAL5 4.5 11.9 1.0 O J:VAL5 4.5 11.9 1.0 CG C:LEU103 4.5 13.6 1.0 CA J:CYS7 4.5 11.4 1.0 CA B:ARG780 4.6 11.3 1.0 C B:ARG780 4.7 11.3 1.0 CA J:PRO4 4.8 11.5 1.0 N J:PHE8 4.8 11.2 1.0

Zinc binding site 7 out of 7 in the Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Cryo-Em Structure of the Rna Polymerase III-MAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ L:Zn101 b:95.2 occ:1.00 SG L:CYS31 2.4 51.2 1.0 SG L:CYS48 2.5 64.7 1.0 CB L:CYS34 2.5 59.2 1.0 CB L:CYS51 2.5 70.2 1.0 SG L:CYS51 2.9 70.2 1.0 CB L:CYS48 3.0 64.7 1.0 CB L:CYS31 3.4 51.2 1.0 SG L:CYS34 3.4 59.2 1.0 CA L:CYS34 3.7 59.2 1.0 N L:CYS34 3.9 59.2 1.0 OG L:SER36 3.9 56.5 1.0 CA L:CYS51 4.0 70.2 1.0 OD1 L:ASP50 4.3 74.1 1.0 CB L:HIS53 4.5 53.6 1.0 CA L:CYS48 4.5 64.7 1.0 N L:CYS51 4.5 70.2 1.0 C L:CYS34 4.5 59.2 1.0 O L:CYS48 4.7 64.7 1.0 C L:CYS51 4.8 70.2 1.0 N L:SER35 4.8 54.3 1.0 CA L:CYS31 4.8 51.2 1.0 CB L:SER36 4.9 56.5 1.0 C L:CYS48 4.9 64.7 1.0 N L:HIS53 4.9 53.6 1.0

M.K.Vorlaender, F.Baudin, R.D.Moir, R.Wetzel, W.J.H.Hagen, I.M.Willis, C.W.Mueller. Structural Basis For Rna Polymerase III Transcription Repression By MAF1 To Be Published.