Zinc in PDB 3t9x: Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2

The structure of Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2, PDB code: 3t9x was solved by A.H.Ahmed, R.E.Oswald, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.84 / 1.82
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	47.239, 114.223, 163.764, 90.00, 90.00, 90.00
R / Rfree (%)	19.9 / 24

The binding sites of Zinc atom in the Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2 (pdb code 3t9x). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2, PDB code: 3t9x:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1 b:28.0 occ:1.00 OE1 B:GLU42 2.2 25.8 1.0 NE2 B:HIS46 2.3 25.8 1.0 CD B:GLU42 3.1 30.9 1.0 CE1 B:HIS46 3.2 32.8 1.0 CD2 B:HIS46 3.2 27.9 1.0 OE2 B:GLU42 3.3 26.9 1.0 ND1 B:HIS46 4.3 30.1 1.0 CG B:HIS46 4.4 27.0 1.0 CD2 B:LEU241 4.5 26.9 1.0 CG B:GLU42 4.5 23.2 1.0 CB B:GLU42 4.9 21.2 1.0

Zinc binding site 2 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn3 b:38.7 occ:1.00 NE2 B:HIS23 2.3 34.1 1.0 CD2 B:HIS23 3.2 33.5 1.0 CE1 B:HIS23 3.4 34.6 1.0 CG B:HIS23 4.4 34.9 1.0 ND1 B:HIS23 4.4 30.8 1.0 CG B:MET19 4.4 38.9 1.0 CB B:MET19 4.7 30.7 1.0

Zinc binding site 3 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn2 b:30.2 occ:1.00 NE2 D:HIS46 2.2 26.5 1.0 OE1 D:GLU42 2.3 26.8 1.0 CD D:GLU42 3.0 29.7 1.0 OE2 D:GLU42 3.1 33.5 1.0 CE1 D:HIS46 3.2 30.7 1.0 CD2 D:HIS46 3.2 28.0 1.0 CD2 D:LEU241 3.6 28.1 1.0 OE1 D:GLN244 3.6 37.8 1.0 CG D:GLU42 4.3 26.3 1.0 ND1 D:HIS46 4.3 32.7 1.0 CG D:HIS46 4.3 27.1 1.0 CD D:GLN244 4.6 39.8 1.0 CB D:GLU42 4.7 23.1 1.0 NE2 D:GLN244 4.8 37.3 1.0

Zinc binding site 4 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn262 b:38.6 occ:1.00 OE2 F:GLU24 2.2 46.8 1.0 OE2 D:GLU30 2.2 40.1 1.0 CD F:GLU24 2.3 48.8 1.0 NE2 D:HIS23 2.4 32.5 1.0 OE1 F:GLU24 2.5 48.7 1.0 NE2 F:HIS23 2.8 42.7 1.0 CE1 D:HIS23 3.2 34.5 1.0 CD D:GLU30 3.2 35.3 1.0 CD2 F:HIS23 3.3 42.6 1.0 CD2 D:HIS23 3.3 32.8 1.0 CG F:GLU24 3.4 53.1 1.0 OE1 D:GLU30 3.5 35.1 1.0 CD D:LYS20 3.5 33.9 1.0 NZ D:LYS20 3.6 36.3 1.0 O D:HOH825 3.9 51.9 1.0 CE1 F:HIS23 4.0 43.6 1.0 CE D:LYS20 4.1 32.5 1.0 CB F:GLU24 4.2 53.7 1.0 ND1 D:HIS23 4.2 31.9 1.0 CG D:HIS23 4.4 27.9 1.0 O D:GLU30 4.5 30.7 1.0 CG F:HIS23 4.6 41.2 1.0 CG D:GLU30 4.6 38.5 1.0 CA F:GLU24 4.8 53.2 1.0 ND1 F:HIS23 4.8 37.9 1.0 CG D:LYS20 4.9 26.7 1.0 O F:HOH793 5.0 46.4 1.0

Zinc binding site 5 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Glutamate Bound to A Double Cysteine Mutant (V484C/E657C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn262 b:62.5 occ:1.00 NE2 F:HIS46 2.2 44.5 1.0 OE1 F:GLU42 2.5 43.7 1.0 CE1 F:HIS46 3.2 45.7 1.0 CD2 F:HIS46 3.2 40.3 1.0 CD F:GLU42 3.5 43.9 1.0 OE2 F:GLU42 4.0 49.5 1.0 CD2 F:LEU241 4.0 32.8 1.0 ND1 F:HIS46 4.3 46.8 1.0 CG F:HIS46 4.4 44.1 1.0 CG F:GLU42 4.7 36.8 1.0 CB F:GLU42 4.9 35.4 1.0 CG F:LYS45 4.9 42.5 1.0

A.H.Ahmed, S.Wang, H.H.Chuang, R.E.Oswald. Mechanism of Ampa Receptor Activation By Partial Agonists: Disulfide Trapping of Closed Lobe Conformations. J.Biol.Chem. V. 286 35257 2011.
ISSN: ISSN 0021-9258
PubMed: 21846932
DOI: 10.1074/JBC.M111.269001