Zinc in PDB 3t93: Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2

The structure of Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2, PDB code: 3t93 was solved by A.H.Ahmed, R.E.Oswald, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.95 / 1.91
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	47.551, 114.253, 163.804, 90.00, 90.00, 90.00
R / Rfree (%)	18.1 / 22.9

The binding sites of Zinc atom in the Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2 (pdb code 3t93). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 5 binding sites of Zinc where determined in the Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2, PDB code: 3t93:
Jump to Zinc binding site number: 1; 2; 3; 4; 5;

Zinc binding site 1 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1 b:23.5 occ:1.00 NE2 B:HIS23 2.3 22.2 1.0 CD2 B:HIS23 3.1 18.0 1.0 CE1 B:HIS23 3.4 21.2 1.0 O B:HOH704 3.6 30.3 1.0 CG B:HIS23 4.4 16.6 1.0 ND1 B:HIS23 4.5 18.4 1.0 CG B:MET19 4.6 24.9 1.0 CB B:MET19 4.7 15.7 1.0

Zinc binding site 2 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2 b:18.3 occ:1.00 OE1 B:GLU42 2.2 15.9 1.0 NE2 B:HIS46 2.3 16.5 1.0 OE1 F:GLU166 2.4 35.9 1.0 OE2 F:GLU166 2.5 19.6 1.0 CD F:GLU166 2.7 21.4 1.0 CD B:GLU42 3.0 17.9 1.0 OE2 B:GLU42 3.2 17.2 1.0 CE1 B:HIS46 3.2 20.4 1.0 CD2 B:HIS46 3.4 16.2 1.0 CD2 B:LEU241 4.1 16.1 1.0 CG F:GLU166 4.1 17.4 1.0 N F:SER168 4.2 13.3 1.0 CG B:GLU42 4.4 12.0 1.0 ND1 B:HIS46 4.4 16.0 1.0 O F:ALA165 4.4 12.7 1.0 CG B:HIS46 4.5 15.8 1.0 CB F:GLU166 4.6 18.5 1.0 CB F:SER168 4.7 13.7 1.0 CA F:GLU166 4.7 16.9 1.0 CA F:PRO167 4.8 15.1 1.0 CA F:SER168 4.8 13.8 1.0 CB B:GLU42 4.8 10.2 1.0 C F:PRO167 4.8 16.7 1.0

Zinc binding site 3 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn3 b:23.5 occ:1.00 NE2 D:HIS23 2.4 27.4 1.0 OE2 D:GLU24 2.5 25.0 1.0 CG D:GLU24 3.1 36.9 1.0 CD D:GLU24 3.2 36.4 1.0 CD2 D:HIS23 3.2 24.3 1.0 CE1 D:HIS23 3.4 26.8 1.0 OE1 D:GLU24 4.4 42.5 1.0 CG D:HIS23 4.4 26.7 1.0 ND1 D:HIS23 4.4 22.8 1.0 CB D:GLU24 4.6 35.0 1.0

Zinc binding site 4 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn262 b:54.0 occ:1.00 OE1 D:GLU42 2.3 28.5 1.0 NE2 D:HIS46 2.3 35.0 1.0 CE1 D:HIS46 3.2 35.5 1.0 CD D:GLU42 3.4 35.3 1.0 CD2 D:HIS46 3.4 30.2 1.0 OE2 D:GLU42 3.7 41.9 1.0 CD2 D:LEU241 4.1 22.3 1.0 CD D:LYS45 4.3 37.7 1.0 ND1 D:HIS46 4.4 32.1 1.0 CG D:HIS46 4.5 31.1 1.0 NZ D:LYS45 4.5 49.2 1.0 CG D:GLU42 4.7 24.2 1.0 CB D:GLU42 4.8 20.9 1.0 CE D:LYS45 4.9 43.4 1.0

Zinc binding site 5 out of 5 in the Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Glutamate Bound to A Double Cysteine Mutant (A452C/S652C) of the Ligand Binding Domain of GLUA2 within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn262 b:19.6 occ:1.00 NE2 F:HIS46 2.3 13.4 1.0 OE1 F:GLU42 2.5 17.4 1.0 OE1 F:GLN244 3.0 50.7 1.0 CE1 F:HIS46 3.2 21.4 1.0 CD F:GLU42 3.3 19.7 1.0 OE2 F:GLU42 3.4 21.7 1.0 CD2 F:HIS46 3.4 19.0 1.0 CD2 F:LEU241 3.5 17.2 1.0 CD F:GLN244 4.0 43.4 1.0 NZ F:LYS45 4.2 20.7 1.0 ND1 F:HIS46 4.4 21.3 1.0 NE2 F:GLN244 4.4 47.3 1.0 CG F:HIS46 4.5 14.7 1.0 CG F:GLU42 4.6 15.8 1.0

A.H.Ahmed, S.Wang, H.H.Chuang, R.E.Oswald. Mechanism of Ampa Receptor Activation By Partial Agonists: Disulfide Trapping of Closed Lobe Conformations. J.Biol.Chem. V. 286 35257 2011.
ISSN: ISSN 0021-9258
PubMed: 21846932
DOI: 10.1074/JBC.M111.269001