Zinc in PDB 9e6t: BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca

The structure of BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca, PDB code: 9e6t was solved by J.R.Horton, X.Cheng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.82 / 2.78
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	59.23, 59.23, 247.124, 90, 90, 90
R / Rfree (%)	24.6 / 28.1

The binding sites of Zinc atom in the BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca (pdb code 9e6t). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 6 binding sites of Zinc where determined in the BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca, PDB code: 9e6t:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6;

Zinc binding site 1 out of 6 in the BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn901 b:127.5 occ:1.00 NE2 A:HIS760 2.0 112.3 1.0 NE2 A:HIS764 2.1 132.7 1.0 CD2 A:HIS760 2.3 123.8 1.0 SG A:CYS747 2.3 112.0 1.0 SG A:CYS744 2.3 112.5 1.0 CD2 A:HIS764 2.6 119.5 1.0 CE1 A:HIS764 3.1 122.2 1.0 CB A:CYS747 3.3 120.4 1.0 CE1 A:HIS760 3.3 110.7 1.0 CG A:HIS760 3.6 103.3 1.0 CB A:CYS744 3.7 124.2 1.0 CG A:HIS764 3.7 121.1 1.0 N A:CYS747 3.9 132.1 1.0 ND1 A:HIS764 3.9 116.3 1.0 ND1 A:HIS760 4.1 93.2 1.0 CA A:CYS747 4.2 130.3 1.0 CB A:TYR746 4.7 132.9 1.0 CB A:HIS760 4.8 93.1 1.0 O A:HIS760 4.8 105.3 1.0 C A:CYS747 4.9 133.1 1.0 C A:TYR746 4.9 141.6 1.0

Zinc binding site 2 out of 6 in the BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn902 b:89.7 occ:1.00 NE2 A:HIS788 2.0 89.2 1.0 NE2 A:HIS792 2.1 91.7 1.0 SG A:CYS775 2.3 85.2 1.0 SG A:CYS772 2.4 125.4 1.0 CE1 A:HIS792 2.8 116.4 1.0 CD2 A:HIS788 2.9 97.1 1.0 CE1 A:HIS788 3.1 93.5 1.0 CD2 A:HIS792 3.1 87.1 1.0 CB A:CYS772 3.4 96.0 1.0 CB A:CYS775 3.5 91.8 1.0 N A:CYS775 3.9 108.4 1.0 ND1 A:HIS792 4.0 108.4 1.0 CG A:HIS788 4.0 87.0 1.0 ND1 A:HIS788 4.1 109.1 1.0 CG A:HIS792 4.1 102.2 1.0 CA A:CYS775 4.2 102.8 1.0 C A:LEU774 4.3 107.6 1.0 CB A:LEU774 4.5 102.6 1.0 CB A:TYR777 4.6 99.0 1.0 SD A:MET789 4.7 112.4 1.0 CA A:LEU774 4.8 104.7 1.0 N A:LEU774 4.8 115.6 1.0 C A:CYS775 4.8 98.8 1.0 CA A:CYS772 4.8 96.0 1.0 O A:LEU774 4.9 109.0 1.0

Zinc binding site 3 out of 6 in the BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn903 b:110.7 occ:1.00 NE2 A:HIS818 2.0 111.7 1.0 NE2 A:HIS823 2.1 106.2 1.0 SG A:CYS805 2.3 93.0 1.0 SG A:CYS802 2.3 107.1 1.0 CD2 A:HIS818 3.0 102.6 1.0 CD2 A:HIS823 3.0 105.3 1.0 CE1 A:HIS823 3.0 107.3 1.0 CE1 A:HIS818 3.0 106.0 1.0 CB A:CYS802 3.0 101.3 1.0 N A:CYS805 3.6 106.9 1.0 CB A:CYS805 3.7 84.3 1.0 ND1 A:HIS823 4.1 86.2 1.0 CG A:HIS823 4.1 94.7 1.0 CG A:HIS818 4.1 96.6 1.0 ND1 A:HIS818 4.1 90.9 1.0 CA A:CYS805 4.1 96.1 1.0 CB A:ILE804 4.4 98.1 1.0 CB A:MET807 4.4 100.2 1.0 CA A:CYS802 4.5 103.8 1.0 C A:CYS805 4.5 105.5 1.0 N A:LYS806 4.6 114.6 1.0 N A:MET807 4.6 100.2 1.0 N A:ILE804 4.7 99.8 1.0 C A:ILE804 4.7 114.7 1.0 CA A:ILE804 4.8 100.6 1.0 CD1 A:ILE804 4.8 104.8 1.0

Zinc binding site 4 out of 6 in the BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1001 b:56.9 occ:1.00 NE2 B:HIS764 2.0 65.4 1.0 NE2 B:HIS760 2.1 60.2 1.0 SG B:CYS747 2.3 60.5 1.0 SG B:CYS744 2.3 78.8 1.0 CD2 B:HIS764 2.6 63.6 1.0 CE1 B:HIS760 2.8 75.1 1.0 CB B:CYS747 3.1 56.4 1.0 CD2 B:HIS760 3.1 45.4 1.0 CE1 B:HIS764 3.1 77.1 1.0 N B:CYS747 3.4 62.5 1.0 CB B:CYS744 3.4 64.5 1.0 CA B:CYS747 3.8 58.0 1.0 CG B:HIS764 3.8 56.8 1.0 ND1 B:HIS760 4.0 78.9 1.0 ND1 B:HIS764 4.0 67.1 1.0 CG B:HIS760 4.1 52.1 1.0 CB B:TYR746 4.1 57.8 1.0 C B:TYR746 4.4 60.5 1.0 C B:CYS747 4.6 58.8 1.0 CA B:TYR746 4.7 49.7 1.0 N B:GLY748 4.7 54.6 1.0 N B:TYR746 4.8 57.7 1.0 CA B:CYS744 4.9 60.9 1.0

Zinc binding site 5 out of 6 in the BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1002 b:49.4 occ:1.00 NE2 B:HIS788 2.0 59.5 1.0 NE2 B:HIS792 2.0 60.6 1.0 SG B:CYS775 2.2 38.1 1.0 SG B:CYS772 2.3 64.3 1.0 CD2 B:HIS788 3.0 58.9 1.0 CE1 B:HIS792 3.0 51.1 1.0 CD2 B:HIS792 3.0 55.6 1.0 CE1 B:HIS788 3.0 51.9 1.0 CB B:CYS775 3.3 52.3 1.0 CB B:CYS772 3.4 53.6 1.0 N B:CYS775 3.8 50.5 1.0 CA B:CYS775 4.1 44.8 1.0 CG B:HIS788 4.1 50.2 1.0 ND1 B:HIS788 4.1 48.4 1.0 ND1 B:HIS792 4.1 58.7 1.0 CG B:HIS792 4.2 56.4 1.0 C B:LEU774 4.2 66.5 1.0 CB B:TYR777 4.3 42.9 1.0 CB B:LEU774 4.5 45.7 1.0 O B:LEU774 4.7 58.7 1.0 CA B:LEU774 4.7 49.0 1.0 C B:CYS775 4.7 45.2 1.0 N B:LEU774 4.7 51.8 1.0 CA B:CYS772 4.8 55.8 1.0 SD B:MET789 4.8 74.3 1.0 N B:TYR777 5.0 56.3 1.0

Zinc binding site 6 out of 6 in the BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of BCL11A ZF4-6 in Complex with A Dna Sequence Observed in the Human Globin Locus Containing Motif Tgccca within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1003 b:58.9 occ:1.00 NE2 B:HIS823 2.0 77.2 1.0 NE2 B:HIS818 2.1 52.9 1.0 SG B:CYS805 2.3 75.7 1.0 SG B:CYS802 2.3 61.3 1.0 CD2 B:HIS823 2.8 94.0 1.0 CE1 B:HIS818 2.9 55.1 1.0 CD2 B:HIS818 3.1 58.7 1.0 CE1 B:HIS823 3.2 64.0 1.0 CB B:CYS802 3.5 67.3 1.0 CB B:CYS805 3.5 56.1 1.0 N B:CYS805 3.8 68.8 1.0 CG B:HIS823 4.0 89.3 1.0 ND1 B:HIS818 4.0 56.8 1.0 CG B:HIS818 4.1 71.4 1.0 ND1 B:HIS823 4.2 79.2 1.0 CA B:CYS805 4.3 62.9 1.0 SD B:MET819 4.3 87.7 1.0 CB B:ILE804 4.6 62.7 1.0 CA B:CYS802 4.9 62.2 1.0 CB B:TRP822 4.9 63.4 1.0 C B:ILE804 4.9 65.8 1.0 N B:ILE804 4.9 76.8 1.0 CB B:MET807 5.0 47.5 1.0 C B:CYS805 5.0 67.7 1.0

J.R.Horton, M.Yu, J.Zhou, M.Tran, R.R.Anakal, Y.Lu, R.M.Blumenthal, X.Zhang, Y.Huang, X.Zhang, X.Cheng. Transcription Factor BCL11A Utilizes Two Zinc-Finger Tandem Arrays to Bind Clustered Short Sequence Motifs To Be Published.