Zinc in PDB 8k60: Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr

All present enzymatic activity of Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr:
2.7.7.6;

The structure of Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr (pdb code 8k60). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr, PDB code: 8k60:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1402 b:75.3 occ:1.00 SG D:CYS60 2.4 51.5 1.0 SG D:CYS75 2.4 57.3 1.0 SG D:CYS62 2.4 52.1 1.0 SG D:CYS78 2.4 53.5 1.0 CB D:CYS75 3.4 57.3 1.0 CB D:CYS62 3.5 52.1 1.0 CB D:CYS60 3.6 51.5 1.0 N D:CYS62 3.7 52.1 1.0 N D:CYS78 3.9 53.5 1.0 CB D:ARG77 3.9 61.9 1.0 CB D:CYS78 4.0 53.5 1.0 CA D:CYS62 4.1 52.1 1.0 CB D:LYS64 4.2 55.4 1.0 N D:TYR61 4.4 47.9 1.0 N D:LYS64 4.4 55.4 1.0 CA D:CYS78 4.5 53.5 1.0 O D:LYS64 4.6 55.4 1.0 N D:GLY63 4.6 51.8 1.0 CA D:ARG77 4.7 61.9 1.0 N D:ARG77 4.7 61.9 1.0 C D:CYS62 4.7 52.1 1.0 C D:ARG77 4.7 61.9 1.0 CA D:CYS75 4.8 57.3 1.0 CA D:LYS64 4.8 55.4 1.0 C D:TYR61 4.8 47.9 1.0 CA D:CYS60 4.9 51.5 1.0 C D:CYS60 4.9 51.5 1.0 C D:CYS75 4.9 57.3 1.0 O D:CYS75 4.9 57.3 1.0 CG D:ARG77 4.9 61.9 1.0 C D:LYS64 5.0 55.4 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Streptomyces Coelicolor Transcription Initiation Complex with the Global Transcription Factor Afsr within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn1403 b:54.6 occ:1.00 SG D:CYS972 2.4 39.9 1.0 SG D:CYS969 2.4 40.9 1.0 SG D:CYS962 2.4 45.1 1.0 CB D:CYS886 2.5 53.2 1.0 CB D:CYS969 3.1 40.9 1.0 SG D:CYS886 3.1 53.2 1.0 CB D:CYS972 3.3 39.9 1.0 CB D:CYS962 3.6 45.1 1.0 CA D:CYS886 3.6 53.2 1.0 N D:CYS969 3.8 40.9 1.0 N D:CYS886 3.9 53.2 1.0 CA D:CYS962 3.9 45.1 1.0 CA D:CYS969 4.0 40.9 1.0 N D:CYS972 4.2 39.9 1.0 N D:GLU963 4.3 49.5 1.0 CA D:CYS972 4.3 39.9 1.0 C D:CYS962 4.6 45.1 1.0 C D:CYS969 4.6 40.9 1.0 O D:CYS969 4.7 40.9 1.0 C D:CYS886 4.9 53.2 1.0

J.Shi, Z.Ye, Z.Feng, A.Wen, L.Wang, Z.Zhang, L.Xu, Q.Song, F.Wang, T.Liu, S.Wang, Y.Feng, W.Lin. Structural Insights Into Transcription Activation of the Streptomyces Antibiotic Regulatory Protein, Afsr Iscience V. 27 10421 2024.
ISSN: ESSN 2589-0042
DOI: 10.1016/J.ISCI.2024.110421