Zinc in PDB 8ei7: Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide

All present enzymatic activity of Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide:
2.3.2.26;

The structure of Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide, PDB code: 8ei7 was solved by K.Li, O.S.Tokareva, T.M.Thomson, G.L.Verdine, J.H.Mcgee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.12 / 2.22
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	62.16, 64.3, 103.69, 90, 90.9, 90
R / Rfree (%)	20 / 26.4

The binding sites of Zinc atom in the Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide (pdb code 8ei7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide, PDB code: 8ei7:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn909 b:79.4 occ:1.00 O A:THR799 2.5 29.8 1.0 OH A:TYR587 2.6 53.0 1.0 OD1 A:ASN840 2.6 30.0 1.0 O A:THR837 2.9 37.0 1.0 CE1 A:TYR587 3.4 41.6 1.0 CZ A:TYR587 3.4 38.5 1.0 C A:THR799 3.4 29.3 1.0 CG A:ASN840 3.7 36.3 1.0 CA A:THR799 3.7 28.6 1.0 O2 A:GOL901 3.8 42.9 1.0 CB A:THR799 3.8 29.0 1.0 O A:ILE813 3.8 39.1 1.0 C A:THR837 3.9 33.0 1.0 ND2 A:ASN840 4.1 34.2 1.0 C2 A:GOL901 4.4 40.6 1.0 N A:GLY800 4.6 31.0 1.0 CA A:THR837 4.7 31.1 1.0 OG1 A:THR799 4.7 30.8 1.0 CD1 A:TYR587 4.7 35.6 1.0 CE2 A:TYR587 4.7 38.9 1.0 O1 A:GOL901 4.7 37.8 1.0 N A:CYS838 4.8 27.9 1.0 CB A:THR837 4.8 30.4 1.0 N A:ASN840 4.8 30.7 1.0 CA A:CYS838 4.9 36.8 1.0 CG2 A:THR799 4.9 27.9 1.0 CB A:ASN840 4.9 28.9 1.0 CA A:GLY814 5.0 34.5 1.0 C A:ILE813 5.0 41.2 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the WWP2 Hect Domain in Complex with H304, A Helicon Polypeptide within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn907 b:98.4 occ:1.00 OD1 B:ASN840 2.6 56.3 1.0 O B:THR837 2.6 47.2 1.0 O B:THR799 2.7 38.0 1.0 CG B:ASN840 3.5 56.9 1.0 O B:ILE813 3.6 54.5 1.0 C B:THR799 3.7 37.2 1.0 C B:THR837 3.7 47.0 1.0 ND2 B:ASN840 3.9 51.4 1.0 CA B:THR799 4.0 33.9 1.0 CE1 B:TYR587 4.1 48.8 1.0 CB B:THR799 4.3 41.2 1.0 CA B:THR837 4.5 45.0 1.0 CB B:THR837 4.6 38.0 1.0 OH B:TYR587 4.6 57.2 1.0 N B:CYS838 4.6 44.0 1.0 CA B:GLY814 4.7 49.5 1.0 CA B:CYS838 4.7 54.7 1.0 N B:ASN840 4.7 56.6 1.0 C B:ILE813 4.8 51.2 1.0 CB B:ASN840 4.8 56.1 1.0 C B:CYS838 4.8 56.2 1.0 CZ B:TYR587 4.9 52.8 1.0 N B:GLY800 4.9 37.8 1.0 CA B:ASN840 4.9 50.4 1.0 O B:CYS838 4.9 55.4 1.0

K.Li, T.L.Travaline, J.-M.Swiecicki, O.S.Tokareva, T.M.Thomson, G.L.Verdine, J.H.Mcgee. Recognition and Reprogramming of E3 Ubiquitin Ligase Surfaces By Alpha-Helical Peptides Nat Commun 2023.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-023-42395-Z