Zinc in PDB 7txc: HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer

The structure of HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer, PDB code: 7txc was solved by J.R.Horton, R.Ren, X.Cheng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.94 / 3.04
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	114.237, 114.237, 59.146, 90, 90, 120
R / Rfree (%)	19.3 / 20.9

The binding sites of Zinc atom in the HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer (pdb code 7txc). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer, PDB code: 7txc:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn701 b:69.3 occ:1.00 NE2 E:HIS555 2.0 75.6 1.0 NE2 E:HIS551 2.0 73.3 1.0 SG E:CYS538 2.2 83.5 1.0 SG E:CYS535 2.3 85.2 1.0 CE1 E:HIS555 2.8 94.6 1.0 CD2 E:HIS551 2.8 82.0 1.0 CD2 E:HIS555 3.0 91.6 1.0 CE1 E:HIS551 3.2 88.7 1.0 N E:CYS538 3.2 86.5 1.0 CB E:CYS535 3.2 82.9 1.0 CB E:CYS538 3.4 81.9 1.0 O E:CYS538 3.6 100.3 1.0 CA E:CYS538 3.7 82.9 1.0 CB E:ILE537 3.8 86.0 1.0 ND1 E:HIS555 3.9 102.3 1.0 CG E:HIS555 4.0 90.9 1.0 C E:CYS538 4.1 100.1 1.0 CG E:HIS551 4.1 71.7 1.0 ND1 E:HIS551 4.2 82.0 1.0 C E:ILE537 4.2 101.3 1.0 CA E:ILE537 4.4 92.6 1.0 N E:ILE537 4.5 96.0 1.0 CA E:CYS535 4.6 82.3 1.0 CG1 E:ILE537 4.6 85.9 1.0 CG2 E:ILE537 4.7 87.5 1.0 C E:CYS535 4.8 85.0 1.0 O E:CYS535 4.9 99.6 1.0 CB E:LYS540 4.9 95.5 1.0

Zinc binding site 2 out of 3 in the HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn702 b:113.4 occ:1.00 NE2 E:HIS523 2.0 99.7 1.0 NE2 E:HIS527 2.1 96.1 1.0 SG E:CYS510 2.2 151.4 1.0 SG E:CYS507 2.3 111.5 1.0 CE1 E:HIS523 2.6 95.6 1.0 CD2 E:HIS527 2.8 116.6 1.0 CB E:CYS507 3.1 135.4 1.0 CD2 E:HIS523 3.1 100.3 1.0 CE1 E:HIS527 3.2 103.4 1.0 ND1 E:HIS523 3.7 93.5 1.0 CB E:CYS510 3.9 143.5 1.0 CG E:HIS523 4.0 96.5 1.0 CG E:LYS512 4.1 115.4 1.0 CG E:HIS527 4.1 106.8 1.0 N E:CYS510 4.1 136.5 1.0 ND1 E:HIS527 4.2 95.9 1.0 CA E:CYS510 4.4 136.5 1.0 O E:CYS510 4.4 137.6 1.0 CB E:LYS512 4.5 119.2 1.0 CA E:CYS507 4.6 134.6 1.0 C E:CYS510 4.7 146.2 1.0 CB E:VAL509 4.8 125.4 1.0 O E:HIS523 4.9 87.0 1.0 CA E:GLU524 4.9 93.4 1.0

Zinc binding site 3 out of 3 in the HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of HIC2 Zinc Finger Domain in Complex with the Dna Binding Motif-2 of the BCL11A Enhancer within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn703 b:198.8 occ:1.00 NE2 E:HIS579 2.1 165.8 1.0 NE2 E:HIS583 2.1 193.7 1.0 SG E:CYS566 2.3 172.9 1.0 SG E:CYS563 2.3 139.0 1.0 CB E:CYS566 2.8 128.2 1.0 CD2 E:HIS579 2.8 167.5 1.0 CE1 E:HIS583 3.0 185.7 1.0 CD2 E:HIS583 3.1 190.2 1.0 CE1 E:HIS579 3.1 163.4 1.0 N E:CYS566 3.5 159.5 1.0 CA E:CYS566 3.7 152.2 1.0 CB E:CYS563 3.8 151.7 1.0 CG E:HIS579 3.9 153.9 1.0 ND1 E:HIS579 4.0 155.7 1.0 ND1 E:HIS583 4.1 184.4 1.0 CG E:HIS583 4.2 187.0 1.0 CG1 E:VAL582 4.4 163.9 1.0 O E:HIS579 4.5 170.6 1.0 C E:GLU565 4.6 156.5 1.0 CB E:GLU565 4.7 141.8 1.0 C E:CYS566 5.0 151.0 1.0

P.Huang, S.A.Peslak, R.Ren, E.Khandros, K.Qin, C.A.Keller, B.Giardine, H.W.Bell, X.Lan, M.Sharma, J.R.Horton, O.Abdulmalik, S.T.Chou, J.Shi, M.Crossley, R.C.Hardison, X.Cheng, G.A.Blobel. HIC2 Controls Developmental Hemoglobin Switching By Repressing BCL11A Transcription. Nat.Genet. V. 54 1417 2022.
ISSN: ISSN 1061-4036
PubMed: 35941187
DOI: 10.1038/S41588-022-01152-6