Zinc in PDB 7byq: The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination.

All present enzymatic activity of The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination.:
3.5.2.6;

The structure of The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination., PDB code: 7byq was solved by Y.S.Park, L.W.Kang, J.H.Lee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.04 / 1.96
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	121.38, 83.057, 163.581, 90, 110.69, 90
R / Rfree (%)	51.9 / 55.7

The binding sites of Zinc atom in the The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination. (pdb code 7byq). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination., PDB code: 7byq:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:29.9 occ:1.00 NE2 A:HIS91 2.1 24.4 1.0 NE2 A:HIS188 2.1 21.6 1.0 OD2 A:ASP210 2.4 23.0 1.0 O A:HOH539 2.6 28.4 1.0 ND1 A:HIS93 2.7 19.0 1.0 CG A:ASP210 2.8 20.7 1.0 CD2 A:HIS188 3.0 21.1 1.0 CE1 A:HIS188 3.0 21.8 1.0 O A:HOH502 3.0 1.5 0.5 CD2 A:HIS91 3.0 23.3 1.0 CE1 A:HIS91 3.1 23.9 1.0 CB A:ASP210 3.1 20.9 1.0 CE1 A:HIS93 3.5 19.7 1.0 OD1 A:ASP210 3.7 20.9 1.0 CG A:HIS93 3.8 19.1 1.0 CG A:HIS188 3.9 19.8 1.0 ND1 A:HIS188 3.9 20.2 1.0 NE2 A:HIS96 3.9 12.3 1.0 CD2 A:HIS96 4.0 12.2 1.0 ND1 A:HIS91 4.1 22.7 1.0 CG A:HIS91 4.2 20.8 1.0 O A:HOH637 4.2 5.2 1.0 CB A:HIS93 4.2 18.7 1.0 CA A:ASP210 4.6 20.9 1.0 NE2 A:HIS93 4.7 20.4 1.0 CE1 A:HIS96 4.8 11.9 1.0 CD2 A:HIS93 4.9 20.0 1.0 CG A:HIS96 5.0 12.3 1.0 O A:HOH616 5.0 0.5 0.5

Zinc binding site 2 out of 4 in the The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination. within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:34.0 occ:1.00 ND1 B:HIS93 2.1 23.3 1.0 NE2 B:HIS91 2.3 28.9 1.0 NE2 B:HIS188 2.3 30.5 1.0 OD2 B:ASP210 2.4 30.1 1.0 O B:HOH562 2.5 31.1 1.0 CE1 B:HIS93 3.0 23.4 1.0 CG B:HIS93 3.1 20.7 1.0 CE1 B:HIS188 3.2 29.9 1.0 O B:HOH505 3.2 2.9 0.5 CD2 B:HIS91 3.3 26.5 1.0 CE1 B:HIS91 3.3 29.0 1.0 CD2 B:HIS188 3.3 29.5 1.0 O B:HOH617 3.3 20.1 1.0 CG B:ASP210 3.4 29.7 1.0 CB B:HIS93 3.5 18.3 1.0 NE2 B:HIS96 3.6 11.7 1.0 CD2 B:HIS96 3.6 11.4 1.0 CB B:ASP210 3.9 28.7 1.0 OD1 B:ASP95 3.9 13.8 1.0 NE2 B:HIS93 4.1 22.0 1.0 CD2 B:HIS93 4.2 21.4 1.0 OD1 B:ASP210 4.3 32.2 1.0 ND1 B:HIS188 4.3 29.7 1.0 CG B:HIS188 4.4 27.9 1.0 ND1 B:HIS91 4.4 26.7 1.0 CG B:HIS91 4.4 23.8 1.0 CE1 B:HIS96 4.7 11.3 1.0 OD2 B:ASP95 4.7 12.7 1.0 CG B:ASP95 4.7 13.7 1.0 CG B:HIS96 4.7 11.6 1.0 CA B:HIS93 4.9 15.9 1.0

Zinc binding site 3 out of 4 in the The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination. within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn401 b:45.8 occ:1.00 ND1 C:HIS93 2.1 37.4 1.0 NE2 C:HIS188 2.2 33.7 1.0 NE2 C:HIS91 2.2 35.8 1.0 OD2 C:ASP210 2.4 36.2 1.0 O C:HOH601 2.5 0.5 0.5 CE1 C:HIS93 2.9 38.0 1.0 O C:HOH501 3.0 3.4 0.5 CD2 C:HIS188 3.1 31.4 1.0 CG C:HIS93 3.1 34.5 1.0 CD2 C:HIS91 3.2 35.2 1.0 CE1 C:HIS91 3.2 37.5 1.0 CE1 C:HIS188 3.3 32.9 1.0 CG C:ASP210 3.4 34.3 1.0 CB C:HIS93 3.6 30.6 1.0 CB C:ASP210 3.7 34.7 1.0 NE2 C:HIS96 3.9 14.1 1.0 NE2 C:HIS93 4.0 37.6 1.0 CD2 C:HIS93 4.1 35.0 1.0 CD2 C:HIS96 4.1 14.6 1.0 O C:HOH600 4.2 13.3 1.0 CG C:HIS188 4.3 30.1 1.0 ND1 C:HIS91 4.3 34.0 1.0 CG C:HIS91 4.3 31.1 1.0 ND1 C:HIS188 4.3 32.2 1.0 OD1 C:ASP210 4.5 34.7 1.0 OD1 C:ASP95 4.7 16.8 1.0 O C:HOH556 4.7 11.2 1.0

Zinc binding site 4 out of 4 in the The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of The Mutant Variant of Pngm-1. H279A Was Substituted For Alanine to Study Metal Coordination. within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn401 b:43.0 occ:1.00 O D:HOH586 2.3 2.4 0.5 OD2 D:ASP210 2.4 34.5 1.0 O D:HOH501 2.5 29.6 0.5 O D:HOH620 2.6 40.1 1.0 O D:HOH602 2.6 39.9 0.5 ND1 D:HIS93 2.7 15.4 1.0 NE2 D:HIS188 2.9 36.4 1.0 CE1 D:HIS93 3.1 15.2 1.0 CG D:ASP210 3.4 32.7 1.0 CE1 D:HIS188 3.5 35.2 1.0 NE2 D:HIS91 3.5 17.9 1.0 CG D:HIS93 3.6 15.2 1.0 NE2 D:HIS93 4.0 15.2 1.0 CB D:ASP210 4.0 31.5 1.0 NE2 D:HIS96 4.0 14.0 1.0 CD2 D:HIS188 4.1 34.2 1.0 O D:HOH600 4.1 9.2 0.5 CD2 D:HIS93 4.2 15.4 1.0 CB D:HIS93 4.2 16.2 1.0 OD1 D:ASP210 4.3 32.0 1.0 CD2 D:HIS96 4.3 13.5 1.0 O D:HOH611 4.4 0.5 0.5 CD2 D:HIS91 4.4 17.1 1.0 CE1 D:HIS91 4.5 17.9 1.0 ND1 D:HIS188 4.7 35.1 1.0

Y.S.Park, L.W.Kang, J.H.Lee. Structural Study of Metal Binding and Coordination of Ancient Metallo Beta Lactamase Pngm 1 Variants To Be Published.