Zinc in PDB 7bre: The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5

All present enzymatic activity of The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5:
2.1.1.354;

The structure of The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5, PDB code: 7bre was solved by Y.Li, L.Zhao, Y.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.83 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	79.075, 66.195, 83.734, 90.00, 93.23, 90.00
R / Rfree (%)	21.9 / 26.5

The binding sites of Zinc atom in the The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5 (pdb code 7bre). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5, PDB code: 7bre:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2801 b:42.5 occ:1.00 SG B:CYS2703 2.3 63.6 1.0 SG B:CYS2705 2.3 51.3 1.0 SG B:CYS2655 2.4 56.0 1.0 SG B:CYS2710 2.4 51.8 1.0 CB B:CYS2703 3.2 65.2 1.0 CB B:CYS2710 3.5 60.0 1.0 CB B:CYS2705 3.6 46.2 1.0 CB B:CYS2655 3.9 56.6 1.0 N B:CYS2655 3.9 55.9 1.0 N B:ARG2711 4.1 61.1 1.0 CA B:CYS2710 4.1 66.6 1.0 N B:CYS2705 4.2 55.4 1.0 CD2 B:HIS2653 4.5 42.1 1.0 C B:CYS2710 4.5 63.9 1.0 CA B:CYS2703 4.5 65.0 1.0 NE2 B:HIS2653 4.5 43.1 1.0 CA B:CYS2705 4.5 54.4 1.0 CA B:CYS2655 4.5 57.9 1.0 N B:ARG2712 4.6 68.5 1.0 C B:CYS2703 4.6 66.8 1.0 C B:SER2654 4.7 50.0 1.0 N B:ASN2704 4.8 66.2 1.0 CA B:SER2654 4.9 44.4 1.0 O B:CYS2703 4.9 66.5 1.0

Zinc binding site 2 out of 2 in the The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of The Crystal Structure of MLL2 in Complex with ASH2L and RBBP5 within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn2801 b:64.5 occ:1.00 SG E:CYS2703 2.3 55.4 1.0 SG E:CYS2705 2.3 75.0 1.0 SG E:CYS2710 2.4 63.7 1.0 SG E:CYS2655 2.4 63.0 1.0 CB E:CYS2705 2.6 70.7 1.0 CB E:CYS2710 3.2 65.0 1.0 CB E:CYS2703 3.5 54.8 1.0 N E:CYS2655 3.6 58.6 1.0 CB E:CYS2655 3.6 65.9 1.0 N E:CYS2705 3.7 69.5 1.0 CA E:CYS2705 3.8 70.7 1.0 CA E:CYS2710 4.1 65.5 1.0 CA E:CYS2655 4.2 63.8 1.0 CD2 E:HIS2653 4.4 38.9 1.0 NE2 E:HIS2653 4.5 38.6 1.0 C E:SER2654 4.5 51.5 1.0 CA E:SER2654 4.5 48.6 1.0 N E:ARG2711 4.6 69.0 1.0 C E:CYS2703 4.7 64.7 1.0 CA E:CYS2703 4.7 58.2 1.0 C E:CYS2710 4.7 68.9 1.0 C E:CYS2705 4.8 74.7 1.0 N E:ARG2712 4.8 64.8 1.0 N E:ASN2704 4.9 63.5 1.0 C E:ASN2704 4.9 68.6 1.0 O E:CYS2703 5.0 72.6 1.0

Y.Li, L.Zhao, X.Tian, C.Peng, F.Gong, Y.Chen. Crystal Structure of MLL2 Complex Guides the Identification of A Methylation Site on P53 Catalyzed By KMT2 Family Methyltransferases. Structure V. 28 1141 2020.
ISSN: ISSN 0969-2126
PubMed: 32697937
DOI: 10.1016/J.STR.2020.07.002