Zinc in PDB 7asu: Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III

The structure of Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III, PDB code: 7asu was solved by A.Vannini, G.Abascal-Palacios, E.P.Ramsay, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.97 / 2.23
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	56.3, 56.3, 275.88, 90, 90, 120
R / Rfree (%)	18 / 22

The binding sites of Zinc atom in the Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III (pdb code 7asu). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III, PDB code: 7asu:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn801 b:66.1 occ:1.00 O A:HOH980 2.1 44.2 1.0 NE2 A:HIS388 2.1 43.9 1.0 O A:HOH984 2.4 44.4 1.0 O A:HOH988 2.5 32.6 1.0 CD2 A:HIS388 3.0 34.6 1.0 CE1 A:HIS388 3.2 36.1 1.0 CG A:HIS388 4.2 38.8 1.0 O A:HOH996 4.2 46.1 1.0 ND1 A:HIS388 4.2 40.5 1.0 O A:HOH1012 4.7 77.3 1.0 O A:HOH942 4.9 81.6 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn802 b:113.9 occ:1.00 O A:HOH976 2.7 61.6 1.0 OD1 A:ASP381 3.0 51.0 1.0 OD2 A:ASP381 3.1 52.1 1.0 CG A:ASP381 3.5 49.1 1.0 CG2 A:VAL344 3.6 58.0 1.0 CB A:VAL344 4.4 55.6 1.0 N A:SER342 4.6 116.7 1.0 O A:HOH1011 4.6 61.7 1.0 CD2 A:LEU349 4.7 43.1 1.0 CB A:ASP381 5.0 41.1 1.0

Zinc binding site 3 out of 4 in the Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn803 b:102.4 occ:1.00 OD2 A:ASP354 2.2 50.6 1.0 O A:HOH941 2.8 43.6 1.0 O A:HOH998 3.0 37.9 1.0 CG A:ASP354 3.1 39.2 1.0 O A:HOH915 3.1 35.2 1.0 OD1 A:ASP354 3.2 33.5 1.0 O A:HOH1006 4.0 61.6 1.0 CB A:SER329 4.2 32.4 1.0 O A:HOH965 4.4 63.8 1.0 CB A:ASP354 4.5 40.8 1.0 N A:SER329 4.7 30.7 1.0 CB A:CYS350 4.8 32.0 1.0 OG A:SER329 4.8 38.2 1.0 O A:CYS350 4.9 38.3 1.0 N A:ASP330 4.9 31.4 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of TWHD1 of RPC5 Subunit of Human Rna Polymerase III within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn804 b:75.6 occ:1.00 O A:HOH994 2.2 33.4 1.0 ND1 A:HIS420 2.5 46.0 1.0 CG A:HIS420 3.4 47.6 1.0 CE1 A:HIS420 3.5 46.6 1.0 O A:HOH957 3.6 58.3 1.0 CB A:HIS420 3.6 35.8 1.0 CA A:HIS420 4.1 34.2 1.0 CD2 A:HIS420 4.6 42.4 1.0 NE2 A:HIS420 4.6 49.1 1.0 O A:HOH909 4.7 51.9 1.0

E.P.Ramsay, G.Abascal-Palacios, J.L.Daiss, H.King, J.Gouge, M.Pilsl, F.Beuron, E.Morris, P.Gunkel, C.Engel, A.Vannini. Structure of Human Rna Polymerase III. Nat Commun V. 11 6409 2020.
ISSN: ESSN 2041-1723
PubMed: 33335104
DOI: 10.1038/S41467-020-20262-5