Zinc in PDB 7apl: Trna-Guanine Transglycosylase G87C Mutant Spin-Labeled with Mtsl

All present enzymatic activity of Trna-Guanine Transglycosylase G87C Mutant Spin-Labeled with Mtsl:
2.4.2.29;

The structure of Trna-Guanine Transglycosylase G87C Mutant Spin-Labeled with Mtsl, PDB code: 7apl was solved by D.Nguyen, A.Heine, G.Klebe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.59 / 1.99
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	64.542, 90.947, 167.818, 90.00, 90.00, 90.00
R / Rfree (%)	17.2 / 20.9

The binding sites of Zinc atom in the Trna-Guanine Transglycosylase G87C Mutant Spin-Labeled with Mtsl (pdb code 7apl). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Trna-Guanine Transglycosylase G87C Mutant Spin-Labeled with Mtsl, PDB code: 7apl:

Zinc binding site 1 out of 1 in the Trna-Guanine Transglycosylase G87C Mutant Spin-Labeled with Mtsl


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Trna-Guanine Transglycosylase G87C Mutant Spin-Labeled with Mtsl within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:28.8 occ:1.00 ND1 A:HIS349 2.1 27.4 1.0 SG A:CYS318 2.3 30.6 1.0 SG A:CYS323 2.3 29.0 1.0 SG A:CYS320 2.3 28.3 1.0 CE1 A:HIS349 3.0 30.6 1.0 CB A:CYS318 3.2 28.5 1.0 CB A:CYS323 3.2 29.9 1.0 CG A:HIS349 3.3 28.9 1.0 CB A:CYS320 3.4 31.7 1.0 CB A:HIS349 3.7 24.5 1.0 N A:CYS323 3.9 30.4 1.0 CA A:HIS349 4.1 25.5 1.0 N A:CYS320 4.1 31.4 1.0 CA A:CYS323 4.1 28.7 1.0 NE2 A:HIS349 4.2 31.4 1.0 CA A:CYS320 4.2 29.5 1.0 CD2 A:HIS349 4.3 27.8 1.0 CA A:CYS318 4.5 32.2 1.0 O A:HIS349 4.6 23.7 1.0 C A:CYS318 4.7 33.5 1.0 O A:CYS320 4.7 29.0 1.0 C A:CYS320 4.7 28.2 1.0 C A:HIS349 4.8 23.4 1.0 CB A:VAL322 4.8 24.6 1.0 O A:CYS318 4.8 31.5 1.0 C A:VAL322 4.9 27.1 1.0

D.Abdullin, D.Nguyen, C.A.Heubach, A.Nguyen, T.Pfaffeneder, A.Heine, K.Reuter, F.Diederich, O.Schiemann, G.Klebe. Unraveling A Ligand-Induced Twist of A Homodimeric Enzyme By Pulsed Electron-Electron Double Resonance To Be Published.