Zinc in PDB 7aof: Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex

All present enzymatic activity of Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex:
2.7.7.6;

The structure of Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex (pdb code 7aof). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex, PDB code: 7aof:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1302 b:130.2 occ:1.00 NE2 A:HIS62 2.1 101.1 1.0 SG A:CYS49 2.3 111.5 1.0 SG A:CYS52 2.3 106.5 1.0 SG A:CYS59 2.3 106.9 1.0 CB A:CYS49 2.8 111.5 1.0 CE1 A:HIS62 3.0 101.1 1.0 CD2 A:HIS62 3.0 101.1 1.0 CB A:CYS59 3.4 106.9 1.0 CB A:CYS52 3.5 106.5 1.0 N A:CYS52 3.8 106.5 1.0 ND1 A:HIS62 4.1 101.1 1.0 CG A:HIS62 4.2 101.1 1.0 CA A:CYS52 4.2 106.5 1.0 CA A:CYS49 4.3 111.5 1.0 CA A:CYS59 4.4 106.9 1.0 CA A:GLY42 4.4 107.9 1.0 CB A:THR51 4.6 103.8 1.0 N A:GLY61 4.8 103.1 1.0 O A:LEU41 4.8 105.4 1.0 C A:THR51 4.8 103.8 1.0 C A:CYS49 4.9 111.5 1.0 N A:GLY53 4.9 108.8 1.0 C A:CYS52 4.9 106.5 1.0 CA A:GLY61 4.9 103.1 1.0 N A:THR51 5.0 103.8 1.0

Zinc binding site 2 out of 4 in the Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1303 b:175.2 occ:1.00 SG A:CYS130 2.3 141.1 1.0 SG A:CYS135 2.3 144.0 1.0 SG A:CYS90 2.3 135.8 1.0 SG A:CYS93 2.3 139.7 1.0 CB A:CYS135 3.1 144.0 1.0 CB A:CYS130 3.3 141.1 1.0 CB A:CYS90 3.9 135.8 1.0 CB A:CYS93 3.9 139.7 1.0 N A:CYS93 4.0 139.7 1.0 CB A:HIS92 4.2 138.6 1.0 CB A:ASN132 4.5 140.9 1.0 CA A:CYS135 4.6 144.0 1.0 CA A:CYS93 4.6 139.7 1.0 O A:CYS90 4.6 135.8 1.0 C A:HIS92 4.6 138.6 1.0 CA A:CYS130 4.6 141.1 1.0 N A:HIS92 4.7 138.6 1.0 CA A:HIS92 4.7 138.6 1.0 ND2 A:ASN132 4.8 140.9 1.0 C A:CYS90 5.0 135.8 1.0 C A:CYS135 5.0 144.0 1.0

Zinc binding site 3 out of 4 in the Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1201 b:136.2 occ:1.00 SG B:CYS1103 2.3 98.4 1.0 SG B:CYS1090 2.3 101.2 1.0 SG B:CYS1106 2.3 102.2 1.0 SG B:CYS1087 2.3 98.8 1.0 CB B:CYS1087 3.3 98.8 1.0 CB B:CYS1106 3.4 102.2 1.0 CB B:CYS1090 3.5 101.2 1.0 CB B:CYS1103 3.6 98.4 1.0 N B:CYS1090 3.7 101.2 1.0 N B:CYS1106 4.0 102.2 1.0 CA B:CYS1090 4.2 101.2 1.0 CA B:CYS1106 4.3 102.2 1.0 CB B:ASN1089 4.3 99.7 1.0 CG B:PRO1113 4.5 100.8 1.0 ND2 B:ASN1089 4.7 99.7 1.0 CA B:CYS1087 4.7 98.8 1.0 C B:ASN1089 4.7 99.7 1.0 CB B:PRO1113 4.8 100.8 1.0 CB B:ARG1105 4.8 102.2 1.0 C B:CYS1090 4.9 101.2 1.0 N B:ASN1089 4.9 99.7 1.0 N B:GLY1091 4.9 97.3 1.0 CA B:ASN1089 4.9 99.7 1.0 CA B:CYS1103 4.9 98.4 1.0 CG B:ASN1089 4.9 99.7 1.0 CB B:ASP1092 5.0 99.1 1.0

Zinc binding site 4 out of 4 in the Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Atomic Structure of the Poxvirus Transcription Late Pre-Initiation Complex within 5.0Å range: probe atom residue distance (Å) B Occ I:Zn801 b:187.6 occ:1.00 N I:CYS366 2.0 142.5 1.0 NE2 I:HIS328 2.1 143.5 1.0 CB I:CYS366 2.3 142.5 1.0 SG I:CYS366 2.3 142.5 1.0 SG I:CYS363 2.3 136.0 1.0 SG I:CYS326 2.3 149.5 1.0 CA I:CYS366 2.5 142.5 1.0 CD2 I:HIS328 2.7 143.5 1.0 C I:VAL365 3.1 143.3 1.0 CE1 I:HIS328 3.3 143.5 1.0 C I:CYS366 3.3 142.5 1.0 CB I:VAL365 3.4 143.3 1.0 CB I:CYS363 3.4 136.0 1.0 O I:CYS366 3.6 142.5 1.0 CB I:CYS326 3.6 149.5 1.0 CA I:VAL365 3.6 143.3 1.0 CG I:HIS328 3.9 143.5 1.0 N I:VAL365 3.9 143.3 1.0 CG1 I:VAL365 4.0 143.3 1.0 O I:VAL365 4.1 143.3 1.0 ND1 I:HIS328 4.2 143.5 1.0 N I:GLY367 4.3 126.2 1.0 CG2 I:VAL365 4.6 143.3 1.0 CA I:CYS363 4.7 136.0 1.0 C I:CYS363 4.9 136.0 1.0 C I:ARG364 5.0 134.9 1.0 CA I:CYS326 5.0 149.5 1.0

C.Grimm, J.Bartuli, B.Boettcher, A.A.Szalay, U.Fischer. Structural Basis of the Complete Poxvirus Transcription Initiation Process Nat.Struct.Mol.Biol. 2021.
ISSN: ESSN 1545-9985
DOI: 10.1038/S41594-021-00655-W