Zinc in PDB 6w7z: RNF12 Ring Domain in Complex with UBE2D2

Enzymatic activity of RNF12 Ring Domain in Complex with UBE2D2

All present enzymatic activity of RNF12 Ring Domain in Complex with UBE2D2:
2.3.2.23; 2.3.2.24; 2.3.2.27;

Protein crystallography data

The structure of RNF12 Ring Domain in Complex with UBE2D2, PDB code: 6w7z was solved by A.J.Middleton, C.L.Day, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.74 / 1.80
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 48.441, 50.453, 54.375, 90.00, 99.72, 90.00
R / Rfree (%) 17.8 / 21.1

Zinc Binding Sites:

The binding sites of Zinc atom in the RNF12 Ring Domain in Complex with UBE2D2 (pdb code 6w7z). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the RNF12 Ring Domain in Complex with UBE2D2, PDB code: 6w7z:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 6w7z

Go back to Zinc Binding Sites List in 6w7z
Zinc binding site 1 out of 2 in the RNF12 Ring Domain in Complex with UBE2D2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of RNF12 Ring Domain in Complex with UBE2D2 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn701

b:22.8
occ:1.00
ND1 B:HIS590 2.1 20.2 1.0
SG B:CYS588 2.3 23.1 1.0
SG B:CYS610 2.3 23.2 1.0
SG B:CYS607 2.4 20.3 1.0
CE1 B:HIS590 2.9 22.5 1.0
CG B:HIS590 3.1 18.9 1.0
CB B:CYS607 3.1 20.9 1.0
CB B:CYS588 3.2 26.5 1.0
CB B:CYS610 3.4 22.6 1.0
CB B:HIS590 3.6 22.9 1.0
N B:CYS610 3.8 21.1 1.0
NE2 B:HIS590 4.1 22.8 1.0
CA B:CYS610 4.2 22.3 1.0
CD2 B:HIS590 4.2 22.6 1.0
CA B:CYS588 4.5 23.6 1.0
N B:HIS590 4.5 25.3 1.0
O B:CYS588 4.5 31.0 1.0
C B:CYS588 4.6 29.3 1.0
CA B:CYS607 4.6 19.5 1.0
CA B:HIS590 4.7 21.0 1.0
CB B:ILE609 4.7 17.9 1.0
NH1 B:ARG612 4.8 24.8 1.0
C B:CYS610 4.8 23.9 1.0
OH B:TYR592 4.8 21.8 1.0
O B:HOH836 4.8 38.8 1.0
C B:ILE609 4.8 20.4 1.0
N B:ARG611 4.9 21.7 1.0
N B:ILE609 5.0 20.0 1.0

Zinc binding site 2 out of 2 in 6w7z

Go back to Zinc Binding Sites List in 6w7z
Zinc binding site 2 out of 2 in the RNF12 Ring Domain in Complex with UBE2D2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of RNF12 Ring Domain in Complex with UBE2D2 within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn702

b:23.8
occ:1.00
ND1 B:HIS593 2.2 23.8 1.0
SG B:CYS596 2.3 26.6 1.0
SG B:CYS573 2.3 24.5 1.0
SG B:CYS570 2.4 22.7 1.0
CB B:CYS570 3.0 26.2 1.0
CG B:HIS593 3.1 25.8 1.0
CE1 B:HIS593 3.3 31.2 1.0
CB B:HIS593 3.3 21.9 1.0
CB B:CYS573 3.3 24.4 1.0
CB B:CYS596 3.4 22.4 1.0
N B:CYS573 3.8 24.7 1.0
N B:HIS593 4.1 20.3 1.0
CA B:CYS573 4.2 23.9 1.0
CD2 B:HIS593 4.3 27.3 1.0
CA B:HIS593 4.3 21.9 1.0
NE2 B:HIS593 4.3 27.8 1.0
CA B:CYS570 4.5 27.1 1.0
CE1 B:HIS595 4.5 59.7 1.0
CB B:VAL572 4.6 21.0 1.0
CA B:CYS596 4.8 28.5 1.0
C B:VAL572 4.8 20.9 1.0
C B:CYS570 4.8 29.4 1.0
NE2 B:HIS595 4.8 59.3 1.0
O B:CYS570 4.8 26.2 1.0
N B:VAL572 4.9 18.8 1.0
C B:CYS573 5.0 25.4 1.0

Reference:

A.J.Middleton, J.Zhu, C.L.Day. The Ring Domain of Ring Finger 12 Efficiently Builds Degradative Ubiquitin Chains J.Mol.Biol..
ISSN: ESSN 1089-8638
DOI: 10.1016/J.JMB.2020.05.001
Page generated: Wed Dec 16 13:03:36 2020

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