Zinc in PDB 6uas: Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E199A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaripentaose

Protein crystallography data

The structure of Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E199A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaripentaose, PDB code: 6uas was solved by P.S.Vieira, L.Cabral, P.A.C.R.Costa, C.R.Santos, M.T.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.14 / 1.91
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 36.680, 79.347, 46.152, 90.00, 103.11, 90.00
R / Rfree (%) 15.7 / 19.8

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E199A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaripentaose (pdb code 6uas). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E199A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaripentaose, PDB code: 6uas:

Zinc binding site 1 out of 1 in 6uas

Go back to Zinc Binding Sites List in 6uas
Zinc binding site 1 out of 1 in the Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E199A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaripentaose


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A GH128 (Subgroup I) Endo-Beta-1,3-Glucanase (E199A Mutant) From Amycolatopsis Mediterranei (AMGH128_I) in Complex with Laminaripentaose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn307

b:22.7
occ:1.00
OD2 A:ASP118 1.9 20.3 1.0
OE2 A:GLU114 2.0 20.0 1.0
O A:HOH577 2.3 7.9 1.0
CG A:ASP118 2.8 20.3 1.0
CD A:GLU114 2.9 20.9 1.0
OD1 A:ASP118 3.0 19.2 1.0
CG A:GLU114 3.2 20.3 1.0
O A:HOH493 4.0 32.7 1.0
OE1 A:GLU114 4.1 21.9 1.0
O A:HOH437 4.2 37.7 1.0
CB A:ASP118 4.2 19.9 1.0
NH2 A:ARG156 4.3 24.1 1.0
O A:GLU114 4.5 20.3 1.0
O A:HOH585 4.6 33.6 1.0
CB A:GLU114 4.7 20.6 1.0

Reference:

C.R.Santos, E.A.Lima, F.Mandelli, M.T.Murakami. Structural Insights Into Beta-1,3-Glucan Cleavage By A Glycoside Hydrolase Family Nat.Chem.Biol. 2020.
ISSN: ESSN 1552-4469
DOI: 10.1038/S41589-020-0554-5
Page generated: Wed Dec 16 12:58:22 2020

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