Zinc in PDB 6p1k: Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme

All present enzymatic activity of Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme:
2.7.7.6;

The structure of Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme (pdb code 6p1k). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme, PDB code: 6p1k:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:0.4 occ:1.00 SG J:CYS72 2.4 53.0 1.0 SG J:CYS85 2.4 45.0 1.0 SG J:CYS70 2.5 34.7 1.0 SG J:CYS88 2.7 52.2 1.0 CB J:CYS72 3.3 53.0 1.0 CB J:CYS85 3.7 45.0 1.0 N J:CYS72 3.8 53.0 1.0 CB J:CYS70 4.0 34.7 1.0 CA J:CYS72 4.1 53.0 1.0 CB J:CYS88 4.4 52.2 1.0 N J:CYS88 4.4 52.2 1.0 N J:GLY73 4.4 42.8 1.0 CB J:LYS74 4.5 48.5 1.0 N J:LYS74 4.6 48.5 1.0 N J:LEU71 4.7 40.9 1.0 C J:CYS72 4.8 53.0 1.0 C J:LEU71 5.0 40.9 1.0 N J:LYS87 5.0 51.8 1.0 CA J:CYS88 5.0 52.2 1.0

Zinc binding site 2 out of 2 in the Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Escherichia Coli SIGMA70 Bound Rnap Polymerase Holoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1503 b:54.7 occ:1.00 SG J:CYS888 2.4 34.7 1.0 SG J:CYS814 2.4 40.4 1.0 SG J:CYS895 2.5 13.0 1.0 SG J:CYS898 2.5 6.6 1.0 CB J:CYS814 3.1 40.4 1.0 CB J:CYS888 3.4 34.7 1.0 CB J:CYS898 3.7 6.6 1.0 CA J:CYS888 3.9 34.7 1.0 CB J:CYS895 4.0 13.0 1.0 N J:ASP889 4.4 37.1 1.0 NH2 J:ARG883 4.4 6.6 1.0 CA J:CYS814 4.5 40.4 1.0 C J:CYS888 4.7 34.7 1.0 N J:CYS895 4.7 13.0 1.0 N J:CYS814 4.7 40.4 1.0 CA J:CYS898 4.9 6.6 1.0 N J:CYS898 4.9 6.6 1.0 N J:CYS888 5.0 34.7 1.0 CA J:CYS895 5.0 13.0 1.0

J.Chen, S.Gopalkrishnan, C.Chiu, A.Y.Chen, E.A.Campbell, R.L.Gourse, W.Ross, S.A.Darst. E. Colitrar Allosterically Regulates Transcription Initiation By Altering Rna Polymerase Conformation. Elife V. 8 2019.
ISSN: ESSN 2050-084X
PubMed: 31841111
DOI: 10.7554/ELIFE.49375