Zinc in PDB 6n9d: Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD)

Enzymatic activity of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD)

All present enzymatic activity of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD):
3.4.24.17;

Protein crystallography data

The structure of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD), PDB code: 6n9d was solved by M.Raeeszadeh-Sarmazdeh, E.S.Radisky, B.Sankaran, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.99 / 2.67
Space group P 65 2 2
Cell size a, b, c (Å), α, β, γ (°) 70.031, 70.031, 319.512, 90.00, 90.00, 120.00
R / Rfree (%) 18.4 / 25.8

Other elements in 6n9d:

The structure of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD) also contains other interesting chemical elements:

Calcium (Ca) 3 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD) (pdb code 6n9d). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD), PDB code: 6n9d:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 6n9d

Go back to Zinc Binding Sites List in 6n9d
Zinc binding site 1 out of 2 in the Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn303

b:50.3
occ:1.00
 ND1 A:HIS179 2.0 44.1 1.0
NE2 A:HIS166 2.1 36.2 1.0
CE1 A:HIS151 2.1 43.9 1.0
NE2 A:HIS151 2.3 43.2 1.0
OD2 A:ASP153 2.4 50.5 1.0
CE1 A:HIS179 2.9 46.3 1.0
CE1 A:HIS166 3.0 42.2 1.0
CG A:ASP153 3.0 44.7 1.0
OD1 A:ASP153 3.1 37.2 1.0
CG A:HIS179 3.1 39.2 1.0
CD2 A:HIS166 3.1 36.3 1.0
ND1 A:HIS151 3.3 39.1 1.0
CD2 A:HIS151 3.5 45.4 1.0
CB A:HIS179 3.5 37.0 1.0
CG A:HIS151 4.0 44.2 1.0
O A:TYR155 4.0 41.2 1.0
NE2 A:HIS179 4.0 41.9 1.0
OH A:TYR168 4.1 49.1 1.0
ND1 A:HIS166 4.2 42.0 1.0
CD2 A:HIS179 4.2 36.4 1.0
CG A:HIS166 4.3 29.4 1.0
CB A:ASP153 4.4 38.0 1.0
CZ A:PHE157 4.6 43.2 1.0
CE1 A:TYR168 4.6 38.7 1.0
CE2 A:PHE157 4.7 43.7 1.0
CZ A:TYR168 4.8 41.9 1.0

Zinc binding site 2 out of 2 in 6n9d

Go back to Zinc Binding Sites List in 6n9d
Zinc binding site 2 out of 2 in the Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Complex of Tissue Inhibitor of Metalloproteinases-1 (Timp-1) Mutant (L34G/L133P/L151C/G154A) with Matrix Metalloproteinase-3 Catalytic Domain (Mmp-3CD) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn304

b:36.6
occ:1.00
 NE2 A:HIS211 2.0 35.1 1.0
NE2 A:HIS201 2.0 37.5 1.0
N B:CYS1 2.2 40.1 1.0
NE2 A:HIS205 2.2 43.6 1.0
O B:CYS1 2.4 45.1 1.0
CD2 A:HIS211 2.8 34.9 1.0
CA B:CYS1 2.9 46.1 1.0
C B:CYS1 2.9 44.8 1.0
CE1 A:HIS201 2.9 33.9 1.0
CD2 A:HIS205 3.0 43.1 1.0
CE1 A:HIS211 3.1 38.4 1.0
CD2 A:HIS201 3.1 35.8 1.0
CB B:CYS1 3.3 40.7 1.0
CE1 A:HIS205 3.3 45.4 1.0
CG A:HIS211 4.0 39.5 1.0
ND1 A:HIS201 4.1 34.5 1.0
ND1 A:HIS211 4.1 45.6 1.0
CG A:HIS201 4.2 40.1 1.0
N B:THR2 4.2 44.0 1.0
OE1 A:GLU202 4.2 37.9 1.0
CG A:HIS205 4.3 33.8 1.0
ND1 A:HIS205 4.4 38.5 1.0
O B:SER68 4.6 47.0 1.0
OE2 A:GLU202 4.7 38.5 1.0
CD A:GLU202 4.8 40.6 1.0
CB A:PRO221 4.8 37.7 1.0
CA A:PRO221 4.9 49.5 1.0
SG B:CYS1 4.9 41.1 1.0
OG1 B:THR2 5.0 48.0 1.0

Reference:

M.Raeeszadeh-Sarmazdeh, K.A.Greene, B.Sankaran, G.P.Downey, D.C.Radisky, E.S.Radisky. Directed Evolution of the Metalloproteinase Inhibitor Timp-1 Reveals That Its N- and C-Terminal Domains Cooperate in Matrix Metalloproteinase Recognition. J.Biol.Chem. V. 294 9476 2019.
ISSN: ESSN 1083-351X
PubMed: 31040180
DOI: 10.1074/JBC.RA119.008321
Page generated: Wed Dec 16 12:21:53 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy