Zinc in PDB 6mur: Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System

The binding sites of Zinc atom in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System (pdb code 6mur). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System, PDB code: 6mur:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn801 b:80.0 occ:1.00 SG A:CYS389 2.3 45.7 1.0 SG A:CYS413 2.5 59.1 1.0 SG A:CYS416 2.5 56.8 1.0 SG A:CYS392 2.5 45.8 1.0 N A:CYS392 3.5 45.8 1.0 CB A:CYS416 3.6 56.8 1.0 CB A:CYS392 3.7 45.8 1.0 CB A:CYS389 3.7 45.7 1.0 CB A:CYS413 4.0 59.1 1.0 CA A:CYS392 4.1 45.8 1.0 N A:CYS413 4.3 59.1 1.0 CB A:VAL391 4.3 47.5 1.0 C A:VAL391 4.4 47.5 1.0 OH A:TYR258 4.5 41.8 1.0 O A:CYS389 4.6 45.7 1.0 C A:CYS392 4.6 45.8 1.0 CA A:CYS413 4.7 59.1 1.0 CA A:VAL391 4.7 47.5 1.0 N A:VAL391 4.7 47.5 1.0 CA A:CYS416 4.8 56.8 1.0 O A:CYS413 4.9 59.1 1.0 C A:CYS389 4.9 45.7 1.0 N A:CYS416 4.9 56.8 1.0 CG1 A:VAL391 4.9 47.5 1.0 O A:CYS392 4.9 45.8 1.0 CA A:CYS389 4.9 45.7 1.0

Zinc binding site 2 out of 3 in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn301 b:37.6 occ:1.00 NE2 C:HIS111 2.2 27.3 1.0 SG C:CYS122 2.4 30.5 1.0 SG C:CYS113 2.4 35.3 1.0 SG C:CYS125 2.4 28.1 1.0 CB C:CYS122 2.8 30.5 1.0 CB C:CYS125 3.0 28.1 1.0 CD2 C:HIS111 3.0 27.3 1.0 CE1 C:HIS111 3.1 27.3 1.0 CB C:CYS113 3.4 35.3 1.0 N C:CYS125 3.6 28.1 1.0 N C:CYS113 3.9 35.3 1.0 CA C:CYS125 3.9 28.1 1.0 ND1 C:HIS111 4.1 27.3 1.0 CG C:HIS111 4.1 27.3 1.0 CA C:CYS113 4.1 35.3 1.0 CA C:CYS122 4.3 30.5 1.0 CB C:VAL124 4.6 25.6 1.0 C C:VAL124 4.8 25.6 1.0 C C:VAL112 4.8 30.4 1.0 C C:CYS122 4.8 30.5 1.0 N C:VAL124 5.0 25.6 1.0

Zinc binding site 3 out of 3 in the Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of Csm-Crrna-Target Rna Ternary Complex in Type III- A Crispr-Cas System within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn301 b:65.7 occ:1.00 NE2 D:HIS111 2.2 41.3 1.0 SG D:CYS122 2.4 38.9 1.0 SG D:CYS113 2.5 41.9 1.0 SG D:CYS125 2.5 31.9 1.0 CD2 D:HIS111 2.9 41.3 1.0 CB D:CYS125 3.0 31.9 1.0 CE1 D:HIS111 3.1 41.3 1.0 CB D:CYS122 3.3 38.9 1.0 N D:CYS125 3.6 31.9 1.0 CB D:CYS113 3.7 41.9 1.0 N D:CYS113 3.9 41.9 1.0 CA D:CYS125 3.9 31.9 1.0 CG D:HIS111 4.0 41.3 1.0 ND1 D:HIS111 4.0 41.3 1.0 CB D:VAL124 4.3 31.9 1.0 CA D:CYS113 4.3 41.9 1.0 C D:VAL124 4.6 31.9 1.0 O D:HIS111 4.7 41.3 1.0 C D:VAL112 4.7 40.7 1.0 CA D:CYS122 4.8 38.9 1.0 CA D:VAL124 4.8 31.9 1.0 CG1 D:VAL124 4.9 31.9 1.0 CA D:VAL112 4.9 40.7 1.0 N D:VAL124 4.9 31.9 1.0 C D:HIS111 5.0 41.3 1.0

N.Jia, C.Y.Mo, C.Wang, E.T.Eng, L.A.Marraffini, D.J.Patel. Type III-A Crispr-Cas Csm Complexes: Assembly, Periodic Rna Cleavage, Dnase Activity Regulation, and Autoimmunity. Mol. Cell V. 73 264 2019.
ISSN: ISSN 1097-4164
PubMed: 30503773
DOI: 10.1016/J.MOLCEL.2018.11.007